Expression and functions of galectin-7 in human and murine melanomas.
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| Title: | Expression and functions of galectin-7 in human and murine melanomas. |
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| Authors: | Biron-Pain, Katherine, Grosset, Andrée-Anne, Poirier, Françoise, Gaboury, Louis, St-Pierre, Yves |
| Contributors: | Armand-Frappier Santé Biotechnologie Research Centre (INRS-AFSB), Institut National de la Recherche Scientifique Québec (INRS)-Pasteur Network (Réseau International des Instituts Pasteur), Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Immunologie et en Cancérologie UdeM-Montréal (IRIC), Université de Montréal (UdeM), This work was funded by grants to YSP from the Canadian Institute for Health Research (Grant No. 14 MOP-89697). KBP and AAG were supported by studentships from the Fond de la Recherche en Santé du Québec (FRSQ). FP was supported by a grant from Ligue Contre le Cancer, Comité de Paris. |
| Source: | ISSN: 1932-6203. |
| Publisher Information: | CCSD Public Library of Science |
| Publication Year: | 2013 |
| Subject Terms: | Apoptosis, Biopsy, Early Growth Response Protein 1/genetics, Early Growth Response Protein 1/metabolism, Galectins/genetics, Galectins/metabolism, Gene Expression Regulation, Neoplastic, Genes, Reporter, Humans, Luciferases, Lung Neoplasms/genetics, Lung Neoplasms/metabolism, Lung Neoplasms/secondary, Melanoma/genetics, Melanoma/metabolism, Melanoma/pathology, Mice, Nevus/genetics, Nevus/pathology, RNA, Messenger/genetics, Messenger/metabolism, Skin Neoplasms/genetics, Skin Neoplasms/metabolism, Skin Neoplasms/secondary, MESH: Animals, [SDV.CAN]Life Sciences [q-bio]/Cancer |
| Description: | International audience ; The identification of galectin-7 as a p53-induced gene and its ability to induce apoptosis in many cell types support the hypothesis that galectin-7 has strong antitumor activity. This has been well documented in colon cancer. However, in some cases, such as breast cancer and lymphoma, its high expression level correlates with aggressive subtypes of cancer, suggesting that galectin-7 may have a dual role in cancer progression. In fact, in breast cancer, overexpression of galectin-7 alone is sufficient to promote metastasis to the bone and lung. In the present work, we investigated the expression and function of galectin-7 in melanoma. An analysis of datasets obtained from whole-genome profiling of human melanoma tissues revealed that galectin-7 mRNA was detected in more than 90% of biopsies of patients with nevi while its expression was more rarely found in biopsies collected from patients with malignant melanoma. This frequency, however, was likely due to the presence of normal epidermis tissues in biopsies, as shown our studies at the protein level by immunohistochemical analysis. Using the experimental melanoma B16F1 cell line, we found that melanoma cells can express galectin-7 at the primary tumor site and in lung metastasis. Moreover, we found that overexpression of galectin-7 increased the resistance of melanoma cells to apoptosis while inducing de novo egr-1 expression. Overexpression of galectin-7, however, was insufficient to modulate the growth of tumors induced by the subcutaneous injection of B16F1 cells. It also failed to modulate the dissemination of B16F1 cells to the lung. |
| Document Type: | article in journal/newspaper |
| Language: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/23658821; PUBMED: 23658821; PUBMEDCENTRAL: PMC3643947 |
| DOI: | 10.1371/journal.pone.0063307 |
| Availability: | https://riip.hal.science/pasteur-01130261 https://riip.hal.science/pasteur-01130261v1/document https://riip.hal.science/pasteur-01130261v1/file/journal.pone.0063307.pdf https://doi.org/10.1371/journal.pone.0063307 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: | edsbas.7FDDBE70 |
| Database: | BASE |
Nájsť tento článok vo Web of Science | Abstract: | International audience ; The identification of galectin-7 as a p53-induced gene and its ability to induce apoptosis in many cell types support the hypothesis that galectin-7 has strong antitumor activity. This has been well documented in colon cancer. However, in some cases, such as breast cancer and lymphoma, its high expression level correlates with aggressive subtypes of cancer, suggesting that galectin-7 may have a dual role in cancer progression. In fact, in breast cancer, overexpression of galectin-7 alone is sufficient to promote metastasis to the bone and lung. In the present work, we investigated the expression and function of galectin-7 in melanoma. An analysis of datasets obtained from whole-genome profiling of human melanoma tissues revealed that galectin-7 mRNA was detected in more than 90% of biopsies of patients with nevi while its expression was more rarely found in biopsies collected from patients with malignant melanoma. This frequency, however, was likely due to the presence of normal epidermis tissues in biopsies, as shown our studies at the protein level by immunohistochemical analysis. Using the experimental melanoma B16F1 cell line, we found that melanoma cells can express galectin-7 at the primary tumor site and in lung metastasis. Moreover, we found that overexpression of galectin-7 increased the resistance of melanoma cells to apoptosis while inducing de novo egr-1 expression. Overexpression of galectin-7, however, was insufficient to modulate the growth of tumors induced by the subcutaneous injection of B16F1 cells. It also failed to modulate the dissemination of B16F1 cells to the lung. |
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| DOI: | 10.1371/journal.pone.0063307 |