Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity
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| Názov: | Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity |
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| Autori: | Katarzyna Pajor, Grzegorz Zając, Marco Fusè, Marzena Mach-Liszka, Marta Arczewska, Mariusz Gagoś, Yoshimitsu Onaka, Tomotsumi Fujisawa, Masashi Unno, Malgorzata Baranska, Ewa Machalska |
| Rok vydania: | 2025 |
| Predmety: | Biophysics, Biochemistry, Medicine, Biotechnology, Sociology, Inorganic Chemistry, Computational Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, raman optical activity, mesoscopic structural features, future structural studies, electronic circular dichroism, ecd signal related, ecd ), respectively, chiral supramolecular systems, senses molecular structure, individual amb molecules, supramolecular structure, amb molecules, polyene chain, molecular conformation, complementary methods, aggregated state |
| Popis: | The polyene antifungal amphotericin B (AmB) can form giant helical aggregates. We show that microscopic and mesoscopic structural features of its aggregates can be revealed by Raman optical activity (ROA) and electronic circular dichroism (ECD), respectively. The ROA method, which senses molecular structure more locally, elucidates the conformation of the polyene chain of individual AmB molecules in an aggregated state. In turn, the ECD signal related to exciton coupling effects provides details about the arrangement of AmB molecules in the supramolecular structure. Thus, the use of both complementary methods will be crucial in future structural studies of chiral supramolecular systems. |
| Druh dokumentu: | article in journal/newspaper |
| Jazyk: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Monitoring_the_Molecular_Conformation_of_Individual_Amphotericin_B_Molecules_in_an_Aggregated_State_by_Raman_Optical_Activity/29168127 |
| DOI: | 10.1021/acs.analchem.5c01198.s001 |
| Dostupnosť: | https://doi.org/10.1021/acs.analchem.5c01198.s001 https://figshare.com/articles/journal_contribution/Monitoring_the_Molecular_Conformation_of_Individual_Amphotericin_B_Molecules_in_an_Aggregated_State_by_Raman_Optical_Activity/29168127 |
| Rights: | CC BY-NC 4.0 |
| Prístupové číslo: | edsbas.7A7C8560 |
| Databáza: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | The polyene antifungal amphotericin B (AmB) can form giant helical aggregates. We show that microscopic and mesoscopic structural features of its aggregates can be revealed by Raman optical activity (ROA) and electronic circular dichroism (ECD), respectively. The ROA method, which senses molecular structure more locally, elucidates the conformation of the polyene chain of individual AmB molecules in an aggregated state. In turn, the ECD signal related to exciton coupling effects provides details about the arrangement of AmB molecules in the supramolecular structure. Thus, the use of both complementary methods will be crucial in future structural studies of chiral supramolecular systems. |
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| DOI: | 10.1021/acs.analchem.5c01198.s001 |