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Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity

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Bibliographic Details
Title: Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity
Authors: Katarzyna Pajor, Grzegorz Zając, Marco Fusè, Marzena Mach-Liszka, Marta Arczewska, Mariusz Gagoś, Yoshimitsu Onaka, Tomotsumi Fujisawa, Masashi Unno, Malgorzata Baranska, Ewa Machalska
Publication Year: 2025
Subject Terms: Biophysics, Biochemistry, Medicine, Biotechnology, Sociology, Inorganic Chemistry, Computational Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, raman optical activity, mesoscopic structural features, future structural studies, electronic circular dichroism, ecd signal related, ecd ), respectively, chiral supramolecular systems, senses molecular structure, individual amb molecules, supramolecular structure, amb molecules, polyene chain, molecular conformation, complementary methods, aggregated state
Description: The polyene antifungal amphotericin B (AmB) can form giant helical aggregates. We show that microscopic and mesoscopic structural features of its aggregates can be revealed by Raman optical activity (ROA) and electronic circular dichroism (ECD), respectively. The ROA method, which senses molecular structure more locally, elucidates the conformation of the polyene chain of individual AmB molecules in an aggregated state. In turn, the ECD signal related to exciton coupling effects provides details about the arrangement of AmB molecules in the supramolecular structure. Thus, the use of both complementary methods will be crucial in future structural studies of chiral supramolecular systems.
Document Type: article in journal/newspaper
Language: unknown
Relation: https://figshare.com/articles/journal_contribution/Monitoring_the_Molecular_Conformation_of_Individual_Amphotericin_B_Molecules_in_an_Aggregated_State_by_Raman_Optical_Activity/29168127
DOI: 10.1021/acs.analchem.5c01198.s001
Availability: https://doi.org/10.1021/acs.analchem.5c01198.s001
https://figshare.com/articles/journal_contribution/Monitoring_the_Molecular_Conformation_of_Individual_Amphotericin_B_Molecules_in_an_Aggregated_State_by_Raman_Optical_Activity/29168127
Rights: CC BY-NC 4.0
Accession Number: edsbas.7A7C8560
Database: BASE
Description
Abstract:The polyene antifungal amphotericin B (AmB) can form giant helical aggregates. We show that microscopic and mesoscopic structural features of its aggregates can be revealed by Raman optical activity (ROA) and electronic circular dichroism (ECD), respectively. The ROA method, which senses molecular structure more locally, elucidates the conformation of the polyene chain of individual AmB molecules in an aggregated state. In turn, the ECD signal related to exciton coupling effects provides details about the arrangement of AmB molecules in the supramolecular structure. Thus, the use of both complementary methods will be crucial in future structural studies of chiral supramolecular systems.
DOI:10.1021/acs.analchem.5c01198.s001