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Inorganic ion concentrations in bodyfluids of marine invertebrates exposed to low salinity in the laboratory

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Titel: Inorganic ion concentrations in bodyfluids of marine invertebrates exposed to low salinity in the laboratory
Autoren: Podbielski, Imke Anna, Hiebenthal, Claas, Hajati, Mithra-Christin, Bock, Christian, Bleich, Markus, Melzner, Frank
Verlagsinformationen: PANGAEA
Publikationsjahr: 2023
Bestand: PANGAEA - Data Publisher for Earth & Environmental Science (AWI Bremerhaven / MARUM Bremen)
Schlagwörter: Asterias_rubens_2018, Baltic Sea, Eckernförde Bay, Kattegat, BD, Bromide, Calcium ion, Calculated average/mean values, cellular volume regulation, Chloride, climate change, Date/time end, experiment, Date/time start, Diadumene_lineata_2018, Dredge, benthos, Event label, Flame photometry, Fluoride, Freeze-depression osmometer, Gonotec, Osmomat 030, HAND, Individual code, invertebrates, Ion chromatography, Kieler Bucht, Laboratory experiment, Littorina_littorea_2018
Geographisches Schlagwort: MEDIAN LATITUDE: 54.721204 * MEDIAN LONGITUDE: 10.346574 * SOUTH-BOUND LATITUDE: 54.318611 * WEST-BOUND LONGITUDE: 10.135000 * NORTH-BOUND LATITUDE: 56.402500 * EAST-BOUND LONGITUDE: 11.351111
Beschreibung: Laboratory experiments were conducted in the climate chambers at GEOMAR Helmholtz Centre for Ocean Research Kiel in the time between March and November 2018. Experiments were designed to study the effect of long-term (1 month) exposure to low salinity in osmoconforming invertebrates. The study organisms (Asterias rubens, Mytilus edulis, Littorina littorea, Diadumene lineata, Strongylocentrotus droebachiensis and Psammechinus milliaris) were collected in Kiel Fjord, Eckernförder Bight or the Kattegat from spring to autumn 2018. Organisms were acclimated to climate chamber conditions for 1 week (under habitat salinity, 14˚C, constant aeration) and then subjected to salinity acclimation for 1-2 weeks until the final salinity treatment level was reached. Then different salinity treatments were maintained for 4 weeks. Water physiochemistry (temperature, salinity, pH, nitrite, nitrate, phosphate) was recorded frequently. After the experiment, samples were taken from seawater and body fluids to measure total osmolality (mosmol/kg) with an osmomat and inorganic ions (mmol/l). No body fluid samples were taken from Diadumene lineata as organisms were too small and volumes too low. Anions were measured with a novel protocol via ion chromatography, cations were measured via flame photometry.
Publikationsart: dataset
Dateibeschreibung: text/tab-separated-values, 5216 data points
Sprache: English
Relation: https://doi.org/10.1594/PANGAEA.959692; Podbielski, Imke Anna; Hiebenthal, Claas; Hajati, Mithra-Christin; Bock, Christian; Bleich, Markus; Melzner, Frank (2022): Capacity for Cellular Osmoregulation Defines Critical Salinity of Marine Invertebrates at Low Salinity. Frontiers in Marine Science, 9, 898364, https://doi.org/10.3389/fmars.2022.898364; Podbielski, Imke Anna (2023): Collection, maintenance and acclimation of marine invertebrates for and during salinity tolerance experiment. Christian-Albrechts-Universität zu Kiel, https://download.pangaea.de/reference/118811/attachments/CollectionMaintenanceAcclimation.xlsx; https://doi.pangaea.de/10.1594/PANGAEA.959683; https://doi.org/10.1594/PANGAEA.959683
DOI: 10.1594/PANGAEA.959683
Verfügbarkeit: https://doi.pangaea.de/10.1594/PANGAEA.959683
https://doi.org/10.1594/PANGAEA.959683
Rights: CC-BY-4.0: Creative Commons Attribution 4.0 International ; Access constraints: unrestricted ; info:eu-repo/semantics/openAccess
Dokumentencode: edsbas.68E627C8
Datenbank: BASE
Beschreibung
Abstract:Laboratory experiments were conducted in the climate chambers at GEOMAR Helmholtz Centre for Ocean Research Kiel in the time between March and November 2018. Experiments were designed to study the effect of long-term (1 month) exposure to low salinity in osmoconforming invertebrates. The study organisms (Asterias rubens, Mytilus edulis, Littorina littorea, Diadumene lineata, Strongylocentrotus droebachiensis and Psammechinus milliaris) were collected in Kiel Fjord, Eckernförder Bight or the Kattegat from spring to autumn 2018. Organisms were acclimated to climate chamber conditions for 1 week (under habitat salinity, 14˚C, constant aeration) and then subjected to salinity acclimation for 1-2 weeks until the final salinity treatment level was reached. Then different salinity treatments were maintained for 4 weeks. Water physiochemistry (temperature, salinity, pH, nitrite, nitrate, phosphate) was recorded frequently. After the experiment, samples were taken from seawater and body fluids to measure total osmolality (mosmol/kg) with an osmomat and inorganic ions (mmol/l). No body fluid samples were taken from Diadumene lineata as organisms were too small and volumes too low. Anions were measured with a novel protocol via ion chromatography, cations were measured via flame photometry.
DOI:10.1594/PANGAEA.959683