Effect of Methyl Jasmonic Acid and Tryptophan on Increasing Vincristine Content of Leaf Cell Suspension Culture Catharanthus roseus (L.) G. Don.
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| Titel: | Effect of Methyl Jasmonic Acid and Tryptophan on Increasing Vincristine Content of Leaf Cell Suspension Culture Catharanthus roseus (L.) G. Don. |
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| Autoren: | Khoiriyah, Siti Khoiriyah, Santosa, Djoko, Purwantini, Indah |
| Quelle: | Indonesian Journal of Pharmacy; Article In Press 2025 ; 2338-9486 ; 2338-9427 |
| Verlagsinformationen: | Faculty of Pharmacy, Universitas Gadjah Mada |
| Publikationsjahr: | 2025 |
| Bestand: | Universitas Gadjah Mada: FMIPA-UGM Online Journal |
| Schlagwörter: | Keywords: Catharanthus roseus (L.) G. Don, vincristine, cell suspension cultures, methyl jasmonic acid, tryptophan |
| Beschreibung: | Catharanthus roseus (L.) G. Don known as tapak dara is a medicinal plant that produces alkaloid compounds such as vincristine as an anticancer which has very high economic value and is produced in very small concentrations. This study aims to increase the levels of vincristine in cell suspension cultures of tapak dara leaves by administering methyl jasmonic acid and tryptophan with various concentrations. Tapak dara leaf explants were grown on solid Murashige-Skoog (MS) media supplemented with the addition of 2.4 D 1 mg/L growth regulator and 4 mg/L kinetin. The callus was subcultured and then suspension culture was carried out on liquid MS medium by administering methyl jasmonic acid and tryptophan with various concentrations. Callus growth was carried out by weighing the fresh weight and dry weight of the callus. The vincristine content and assay were analyzed using High Performance Liquid Chromatography (HPLC). Callus growth in cell suspension cultures with X50Y300 treatment resulted in the highest fresh weight of 1.094 g, while the highest dry weight was found in treatment X75Y200 of 0.166 g. The results of the HPLC analysis showed that the administration of methyl jasmonic acid and tryptophan with various concentrations on tapak dara leaf cell suspension cultures were able to produce vincristine compounds. In treatment, X50Y100, X75Y100, X100Y100, X50Y200, X75Y200, and X75Y300 were able to increase vincristine levels, but the X100Y200, X50Y300, and X100Y300 treatments decreased vincristine levels. The highest levels of vincristine were found in treatment X50Y200 (50 mg/L tryptophan and 200 μM methyl jasmonic acid ) which was 49.311 ppm . |
| Publikationsart: | article in journal/newspaper |
| Sprache: | English |
| Relation: | https://jurnal.ugm.ac.id/v3/IJP/article/view/11201 |
| DOI: | 10.22146/ijp.11201 |
| Verfügbarkeit: | https://jurnal.ugm.ac.id/v3/IJP/article/view/11201 https://doi.org/10.22146/ijp.11201 |
| Rights: | Copyright (c) 2025 Indonesian Journal of Pharmacy ; https://creativecommons.org/licenses/by/2.0/ |
| Dokumentencode: | edsbas.5A8F0C0E |
| Datenbank: | BASE |
Nájsť tento článok vo Web of Science | Abstract: | Catharanthus roseus (L.) G. Don known as tapak dara is a medicinal plant that produces alkaloid compounds such as vincristine as an anticancer which has very high economic value and is produced in very small concentrations. This study aims to increase the levels of vincristine in cell suspension cultures of tapak dara leaves by administering methyl jasmonic acid and tryptophan with various concentrations. Tapak dara leaf explants were grown on solid Murashige-Skoog (MS) media supplemented with the addition of 2.4 D 1 mg/L growth regulator and 4 mg/L kinetin. The callus was subcultured and then suspension culture was carried out on liquid MS medium by administering methyl jasmonic acid and tryptophan with various concentrations. Callus growth was carried out by weighing the fresh weight and dry weight of the callus. The vincristine content and assay were analyzed using High Performance Liquid Chromatography (HPLC). Callus growth in cell suspension cultures with X50Y300 treatment resulted in the highest fresh weight of 1.094 g, while the highest dry weight was found in treatment X75Y200 of 0.166 g. The results of the HPLC analysis showed that the administration of methyl jasmonic acid and tryptophan with various concentrations on tapak dara leaf cell suspension cultures were able to produce vincristine compounds. In treatment, X50Y100, X75Y100, X100Y100, X50Y200, X75Y200, and X75Y300 were able to increase vincristine levels, but the X100Y200, X50Y300, and X100Y300 treatments decreased vincristine levels. The highest levels of vincristine were found in treatment X50Y200 (50 mg/L tryptophan and 200 μM methyl jasmonic acid ) which was 49.311 ppm . |
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| DOI: | 10.22146/ijp.11201 |