In silico, in vitro, and in vivo characterization of thiamin-binding proteins from plant seeds
Uložené v:
| Názov: | In silico, in vitro, and in vivo characterization of thiamin-binding proteins from plant seeds |
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| Autori: | Vicente Faustino, Maria, Strobbe, Simon, Sanchez Muñoz, Raul, Cao, Da, Mishra, Ratnesh, Lourenço, Tiago, Oliveira, M. Margarida, Van Der Straeten, Dominique |
| Zdroj: | BIOCHEMICAL JOURNAL ; ISSN: 0264-6021 ; ISSN: 1470-8728 |
| Rok vydania: | 2025 |
| Zbierka: | Ghent University Academic Bibliography |
| Predmety: | Biology and Life Sciences, YEAST SACCHAROMYCES-CEREVISIAE, MULTIPLE SEQUENCE ALIGNMENT, GENE-EXPRESSION, RICE, BIOSYNTHESIS, METABOLISM, RIBOSWITCH, TRANSFORMATION, ACCUMULATION, FUSION |
| Popis: | Thiamin, an essential micronutrient, is a cofactor for enzymes involved in the central carbon metabolism and amino acid pathways. Despite efforts to enhance thiamin content in rice by incorporating thiamin biosynthetic genes, increasing thiamin content in the endosperm remains challenging, possibly due to a lack of thiamin stability and/or a local sink. The introduction of storage proteins has been successful in several biofortification strategies, and similar efforts targeting thiamin have been performed, leading to a 3-4-fold increase in white rice. However, only one thiamin-binding protein (TBP) sequence has been described in plants, more specifically from sesame seeds. Therefore, we aimed to identify and characterize TBPs, as well as to evaluate the effect of their expression on thiamin concentration, using a comprehensive approach integrating in silico, in vitro, and in vivo methods. We identified the sequences of putative TBPs from Oryza sativa (Os, rice), Fagopyrum esculentum (Fe, buckwheat), and Zea mays (Zm, maize) and pinpointed the thiamin-binding pockets through molecular docking. FeTBP and OsTBP contained one pocket with binding affinities similar to the Escherichia coli TBP, a well-characterized TBP, supporting their function as TBPs. In vivo expression studies of TBPs in tobacco leaves and rice callus resulted in increased thiamin levels, with FeTBP and OsTBP showing the most pronounced effects. Additionally, thermal shift assays confirmed the thiamin-binding capabilities of FeTBP and OsTBP, as observed by the significant increases in melting temperatures upon thiamin binding, indicating protein stabilization. These findings offer new insights into the diversity and function of plant TBPs and highlight the potential of FeTBP and OsTBP to modulate thiamin levels in crop plants. |
| Druh dokumentu: | article in journal/newspaper |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| Relation: | https://biblio.ugent.be/publication/01JN3BVEAX3TR2KK1A5AM2QQ20; https://doi.org/10.1042/bcj20240429; https://biblio.ugent.be/publication/01JN3BVEAX3TR2KK1A5AM2QQ20/file/01JPSGG5PWA99KSFJ6DW3NXFV5 |
| DOI: | 10.1042/bcj20240429 |
| Dostupnosť: | https://biblio.ugent.be/publication/01JN3BVEAX3TR2KK1A5AM2QQ20 https://hdl.handle.net/1854/LU-01JN3BVEAX3TR2KK1A5AM2QQ20 https://doi.org/10.1042/bcj20240429 https://biblio.ugent.be/publication/01JN3BVEAX3TR2KK1A5AM2QQ20/file/01JPSGG5PWA99KSFJ6DW3NXFV5 |
| Rights: | info:eu-repo/semantics/openAccess |
| Prístupové číslo: | edsbas.57F160BE |
| Databáza: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | Thiamin, an essential micronutrient, is a cofactor for enzymes involved in the central carbon metabolism and amino acid pathways. Despite efforts to enhance thiamin content in rice by incorporating thiamin biosynthetic genes, increasing thiamin content in the endosperm remains challenging, possibly due to a lack of thiamin stability and/or a local sink. The introduction of storage proteins has been successful in several biofortification strategies, and similar efforts targeting thiamin have been performed, leading to a 3-4-fold increase in white rice. However, only one thiamin-binding protein (TBP) sequence has been described in plants, more specifically from sesame seeds. Therefore, we aimed to identify and characterize TBPs, as well as to evaluate the effect of their expression on thiamin concentration, using a comprehensive approach integrating in silico, in vitro, and in vivo methods. We identified the sequences of putative TBPs from Oryza sativa (Os, rice), Fagopyrum esculentum (Fe, buckwheat), and Zea mays (Zm, maize) and pinpointed the thiamin-binding pockets through molecular docking. FeTBP and OsTBP contained one pocket with binding affinities similar to the Escherichia coli TBP, a well-characterized TBP, supporting their function as TBPs. In vivo expression studies of TBPs in tobacco leaves and rice callus resulted in increased thiamin levels, with FeTBP and OsTBP showing the most pronounced effects. Additionally, thermal shift assays confirmed the thiamin-binding capabilities of FeTBP and OsTBP, as observed by the significant increases in melting temperatures upon thiamin binding, indicating protein stabilization. These findings offer new insights into the diversity and function of plant TBPs and highlight the potential of FeTBP and OsTBP to modulate thiamin levels in crop plants. |
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| DOI: | 10.1042/bcj20240429 |