Zobrazit v EDS

The AMPK-mTOR signaling pathway is involved in regulation of food intake in the hypothalamus of stressed chickens.

Uloženo v:
Podrobná bibliografie
Název: The AMPK-mTOR signaling pathway is involved in regulation of food intake in the hypothalamus of stressed chickens.
Autoři: Hu, Xiyi, Kong, Linglian, Xiao, Chuanpi, Zhu, Qidong, Song, Zhigang
Zdroj: Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology, 258, 110979 (2021-08)
Informace o vydavateli: Elsevier BV
Rok vydání: 2021
Sbírka: University of Liège: ORBi (Open Repository and Bibliography)
Témata: AMPK, Appetite, Broiler, Compound C, Glucocorticoids, Hypothalamus, Dexamethasone, TOR Serine-Threonine Kinases, AMP-Activated Protein Kinases, AMP-Activated Protein Kinases/metabolism, Animals, Behavior, Animal, Chickens, Dexamethasone/pharmacology, Eating/drug effects, Feeding Behavior/drug effects, Gene Expression Regulation/drug effects, Glucocorticoids/metabolism, Hypothalamus/metabolism, Hypothalamus/physiology, Infusions, Intraventricular, Male, Signal Transduction, Stress, Physiological, TOR Serine-Threonine Kinases/metabolism, Molecular Biology, Physiology
Popis: peer reviewed ; Glucocorticoids (GCs) can stimulate the appetite and AMPK in broilers. The activation of hypothalamic mTOR has been proposed as an important anorexigenic signal. However, inhibitory effect of AMPK activity on appetite and AMPK downstream signaling pathway under stress has not been reported. In this study, we performed an intracerebroventricular (icv) injection of compound C, an AMPK inhibitor, in GC-treated birds to explore the regulatory mechanism on appetite and AMPK downstream signaling pathway. A total of 48 7-day-old broilers, which had received an icv cannula, were randomly subjected to one of two treatments: subcutaneous injection of dexamethasone (DEX) or saline. After 3 days of continuous DEX injection, chicks of each group received an icv injection with either compound C (6 μg/2 μL) or vehicle (dimethyl sulfoxide, 2 μL). The results showed that body weight gain was reduced by the DEX treatment. Compared with the control, icv injection of compound C reduced feed intake at 0.5-1.5 h. In the DEX-treated group, the inhibitory effect of compound C on appetite remained apparent at 0.5-1 h. The DEX treatment increased the gene expression of liver kinase B1 (LKB1), neuropeptide Y (NPY), and decreased p-mTOR protein level. In stressed broilers, inhibition of AMPK relieved the decreased mTOR activity. A significant interaction was noted in DEX and compound C on protein expression of phospho-AMPK. Taken together, in stressed broilers, the central injection of compound C could inhibit central AMPK activity and reduce appetite, in which the AMPK/mTOR signaling pathway might be involved.
Druh dokumentu: article in journal/newspaper
Jazyk: English
ISSN: 1095-6433
1531-4332
Relation: https://api.elsevier.com/content/article/PII:S1095643321000854?httpAccept=text/xml; urn:issn:1095-6433; urn:issn:1531-4332; https://orbi.uliege.be/handle/2268/316296; info:hdl:2268/316296; https://orbi.uliege.be/bitstream/2268/316296/1/10.1016j.cbpa.2021.110979.pdf; info:pmid:33991669
DOI: 10.1016/j.cbpa.2021.110979
Dostupnost: https://orbi.uliege.be/handle/2268/316296
https://orbi.uliege.be/bitstream/2268/316296/1/10.1016j.cbpa.2021.110979.pdf
https://doi.org/10.1016/j.cbpa.2021.110979
Rights: open access ; http://purl.org/coar/access_right/c_abf2 ; info:eu-repo/semantics/openAccess
Přístupové číslo: edsbas.5371857
Databáze: BASE
Popis
Abstrakt:peer reviewed ; Glucocorticoids (GCs) can stimulate the appetite and AMPK in broilers. The activation of hypothalamic mTOR has been proposed as an important anorexigenic signal. However, inhibitory effect of AMPK activity on appetite and AMPK downstream signaling pathway under stress has not been reported. In this study, we performed an intracerebroventricular (icv) injection of compound C, an AMPK inhibitor, in GC-treated birds to explore the regulatory mechanism on appetite and AMPK downstream signaling pathway. A total of 48 7-day-old broilers, which had received an icv cannula, were randomly subjected to one of two treatments: subcutaneous injection of dexamethasone (DEX) or saline. After 3 days of continuous DEX injection, chicks of each group received an icv injection with either compound C (6 μg/2 μL) or vehicle (dimethyl sulfoxide, 2 μL). The results showed that body weight gain was reduced by the DEX treatment. Compared with the control, icv injection of compound C reduced feed intake at 0.5-1.5 h. In the DEX-treated group, the inhibitory effect of compound C on appetite remained apparent at 0.5-1 h. The DEX treatment increased the gene expression of liver kinase B1 (LKB1), neuropeptide Y (NPY), and decreased p-mTOR protein level. In stressed broilers, inhibition of AMPK relieved the decreased mTOR activity. A significant interaction was noted in DEX and compound C on protein expression of phospho-AMPK. Taken together, in stressed broilers, the central injection of compound C could inhibit central AMPK activity and reduce appetite, in which the AMPK/mTOR signaling pathway might be involved.
ISSN:10956433
15314332
DOI:10.1016/j.cbpa.2021.110979