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Titanium microbead-based porous implants: bead size controls cell response and host integration.

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Název: Titanium microbead-based porous implants: bead size controls cell response and host integration.
Autoři: Vrana, Engin, Dupret-Bories, Agnès, Schultz, Philippe, Debry, Christian, Vautier, Dominique, Lavalle, Philippe
Přispěvatelé: Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Service d'ORL et chirurgie cervico-faciale, Centre Hospitalier Universitaire Strasbourg (CHU Strasbourg), Hôpitaux Universitaires de Strasbourg (HUS)-Hôpitaux Universitaires de Strasbourg (HUS)-Hôpital de Hautepierre Strasbourg, Hôpitaux Universitaires de Strasbourg (HUS)
Zdroj: Adv Healthc Mater ; https://inserm.hal.science/inserm-00846105 ; Adv Healthc Mater, 2014, 3 (1), pp.79-87. ⟨10.1002/adhm.201200369⟩
Informace o vydavateli: CCSD
Rok vydání: 2014
Sbírka: Inserm: HAL (Institut national de la santé et de la recherche médicale)
Témata: titanium, trachea, host integration, in vivo, porous implants, MESH: Animals, MESH: Cell Movement, MESH: NIH 3T3 Cells, MESH: Particle Size, MESH: Porosity, MESH: Prostheses and Implants, MESH: Rabbits, MESH: Rats, MESH: Titanium, MESH: Cell Proliferation, MESH: Coculture Techniques, MESH: Collagen, MESH: Human Umbilical Vein Endothelial Cells, MESH: Humans, MESH: Infusions, Subcutaneous, MESH: Mice, MESH: Microspheres, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Popis: International audience ; Openly porous structures in implants are desirable for better integration with the host tissue. Sintered microbead-based titanium implants for oto-rhinolaryngology applications, which create an environment where the cells can migrate in the areas between the microbeads, are developed. This structure promotes fibrovascular tissue formation within the implant in vivo. In this study, it is determine to what extent these events can be controlled by changing the physical environment of the implants both in vitro and in vivo. By cell tracking, it is observed that the size of the beads and the distance between the neighboring beads significantly affect the ability of cells to develop cell-to-cell contacts and to bridge the pores. Live cell staining shows that as the bead size gets smaller, the probability to observe cells that fill the porous areas is higher. This also affects the initial attachment and distribution of the cells and collagen secretion by fibroblasts. Obtaining a fast coverage of the system also enables co-culture systems where, the number and the distribution of the second cell type are boosted by the presence of the first. This concept is utilized to increase the attachment of vascular endothelial cells by an initial layer of fibroblasts. By decreasing the bead diameter, the overall colonization of the implant can be significantly increased in vivo. The effect of bead size has a similar pattern both in rats and rabbits, with faster colonization of smaller bead-based structures. Using smaller beads would improve clinical outcomes as faster integration facilitates the attainment of functionality by the implant.
Druh dokumentu: article in journal/newspaper
Jazyk: English
Relation: info:eu-repo/semantics/altIdentifier/pmid/23814016; PUBMED: 23814016
DOI: 10.1002/adhm.201200369
Dostupnost: https://inserm.hal.science/inserm-00846105
https://inserm.hal.science/inserm-00846105v1/document
https://inserm.hal.science/inserm-00846105v1/file/Vraneet_al_2012_Titanium_Bead_SizeAdvanced_Healthcare_materials_after_revision_final.pdf
https://doi.org/10.1002/adhm.201200369
Rights: info:eu-repo/semantics/OpenAccess
Přístupové číslo: edsbas.4EB9FE48
Databáze: BASE
Popis
Abstrakt:International audience ; Openly porous structures in implants are desirable for better integration with the host tissue. Sintered microbead-based titanium implants for oto-rhinolaryngology applications, which create an environment where the cells can migrate in the areas between the microbeads, are developed. This structure promotes fibrovascular tissue formation within the implant in vivo. In this study, it is determine to what extent these events can be controlled by changing the physical environment of the implants both in vitro and in vivo. By cell tracking, it is observed that the size of the beads and the distance between the neighboring beads significantly affect the ability of cells to develop cell-to-cell contacts and to bridge the pores. Live cell staining shows that as the bead size gets smaller, the probability to observe cells that fill the porous areas is higher. This also affects the initial attachment and distribution of the cells and collagen secretion by fibroblasts. Obtaining a fast coverage of the system also enables co-culture systems where, the number and the distribution of the second cell type are boosted by the presence of the first. This concept is utilized to increase the attachment of vascular endothelial cells by an initial layer of fibroblasts. By decreasing the bead diameter, the overall colonization of the implant can be significantly increased in vivo. The effect of bead size has a similar pattern both in rats and rabbits, with faster colonization of smaller bead-based structures. Using smaller beads would improve clinical outcomes as faster integration facilitates the attainment of functionality by the implant.
DOI:10.1002/adhm.201200369