Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array
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| Title: | Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array |
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| Authors: | Victor Moro, Joan Canals, Sergio Moreno, Steffen Higgins-Wood, Oscar Alonso, Andreas Waag, J. Daniel Prades, Angel Dieguez |
| Source: | Biosensors, Vol 14, Iss 6, p 264 (2024) |
| Publisher Information: | MDPI AG |
| Publication Year: | 2024 |
| Collection: | Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: | Point-of-Care, multiplex, microLED array, SPAD, fluorescence, lifetime fluorescence, Biotechnology, TP248.13-248.65 |
| Description: | A Point-of-Care system for molecular diagnosis (PoC-MD) is described, combining GaN and CMOS chips. The device is a micro-system for fluorescence measurements, capable of analyzing both intensity and lifetime. It consists of a hybrid micro-structure based on a 32 × 32 matrix addressable GaN microLED array, with square LEDs of 50 µm edge length and 100 µm pitch, with an underneath wire bonded custom chip integrating their drivers and placed face-to-face to an array of 16 × 16 single-photon avalanche diodes (SPADs) CMOS. This approach replaces instrumentation based on lasers, bulky optical components, and discrete electronics with a full hybrid micro-system, enabling measurements on 32 × 32 spots. The reported system is suitable for long lifetime (>10 ns) fluorophores with a limit of detection ~1/4 µM. Proof-of-concept measurements of streptavidin conjugate Qdot™ 605 and Amino PEG Qdot™ 705 are demonstrated, along with the device ability to detect both fluorophores in the same measurement. |
| Document Type: | article in journal/newspaper |
| Language: | English |
| Relation: | https://www.mdpi.com/2079-6374/14/6/264; https://doaj.org/toc/2079-6374; https://doaj.org/article/224454de92804758bbd7c6d4dfb95dc8 |
| DOI: | 10.3390/bios14060264 |
| Availability: | https://doi.org/10.3390/bios14060264 https://doaj.org/article/224454de92804758bbd7c6d4dfb95dc8 |
| Accession Number: | edsbas.414919D4 |
| Database: | BASE |
Nájsť tento článok vo Web of Science | Abstract: | A Point-of-Care system for molecular diagnosis (PoC-MD) is described, combining GaN and CMOS chips. The device is a micro-system for fluorescence measurements, capable of analyzing both intensity and lifetime. It consists of a hybrid micro-structure based on a 32 × 32 matrix addressable GaN microLED array, with square LEDs of 50 µm edge length and 100 µm pitch, with an underneath wire bonded custom chip integrating their drivers and placed face-to-face to an array of 16 × 16 single-photon avalanche diodes (SPADs) CMOS. This approach replaces instrumentation based on lasers, bulky optical components, and discrete electronics with a full hybrid micro-system, enabling measurements on 32 × 32 spots. The reported system is suitable for long lifetime (>10 ns) fluorophores with a limit of detection ~1/4 µM. Proof-of-concept measurements of streptavidin conjugate Qdot™ 605 and Amino PEG Qdot™ 705 are demonstrated, along with the device ability to detect both fluorophores in the same measurement. |
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| DOI: | 10.3390/bios14060264 |