Enhanced Kidney Targeting and Distribution of Tubuloids During Normothermic Perfusion
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| Název: | Enhanced Kidney Targeting and Distribution of Tubuloids During Normothermic Perfusion |
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| Autoři: | Montagud-Marrahi, Enrique, Rodriguez-Gonzalo, Adriana, López-Aladid, Rubén, Luque, Yosu, Rabadán-Ros, Ruben, Cuadrado-Payan, Elena, Bañón-Maneus, Elisenda, Rovira, Jordi, Lazo-Rodríguez, Marta, Aguilà, Oriol, Arana, Carolt, García-Busquets, Ainhoa, Hierro, Natalia, Prudhomme, Thomas, Musquera, Mireia, Xia, Yun, Diekmann, Fritz, Campistol, Josep, Ramírez-Bajo, Maria José |
| Přispěvatelé: | Clinic Barcelona Hospital Universitari, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona (UB), Red de Investigación Cooperativa Orientada a Resultados en Salud Madrid, Spain (RICORS 2040), CoRaKiD - Maladies rénales fréquentes et rares : des mécanismes moléculaires à la médecine personnalisée (CoRaKID), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Tenon AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Universidad Católica San Antonio de Murcia (UCAM), Centre de Recherche en Transplantation et Immunologie - Center for Research in Transplantation and Translational Immunology (U1064 Inserm - CR2TI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Team 3 : Integrative transplantation, HLA, Immunology and genomics of kidney injury (Team 3 - U1064 Inserm - CR2TI), Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), The Salk Institute for Biological Studies |
| Zdroj: | ISSN: 0934-0874. |
| Informace o vydavateli: | CCSD Frontiers Media |
| Rok vydání: | 2025 |
| Témata: | cell therapy, kidney regeneration, normothermic perfusion, transplantation, tubuloids, MESH: Animals, MESH: Cell Differentiation, MESH: Rats, MESH: Regeneration, MESH: Humans, MESH: Kidney Transplantation* / methods, MESH: Kidney Tubules* / cytology, MESH: Kidney Tubules, Proximal* / cytology, MESH: Kidney, MESH: Male, MESH: Organ Preservation* / methods, MESH: Perfusion* / methods, [SDV]Life Sciences [q-bio] |
| Popis: | International audience ; Tubuloids have become a promising tool for modeling and regenerating kidney disease, although their ability for integration and regeneration in vivo is not well documented. Here, we established, characterized, and compared human tubuloids using two optimized protocols: one involving prior isolation of tubular cells (Crude tubuloids) and the other involving prior isolation of proximal tubular cells (F4 tubuloids). Next, healthy rat-derived tubuloids were established using this protocol. Finally, we compared two strategies for delivering GFP tubuloids to a kidney host: 1) subcapsular/intracortical injection and 2) tubuloid infusion during normothermic preservation in a rat transplantation model and a discarded human kidney. F4 tubuloids achieved a higher level of differentiation state compared to Crude tubuloids. When analyzing tubuloid delivery to the kidney, normothermic perfusion was found to be more efficient than in vivo injection. Moreover, fully developed tubules were observed in the host parenchyma at 1 week and 1 month after infusion during normothermic perfusion represent a potential strategy to enhance the translatability of kidney regenerative therapies into clinical practice to condition kidney grafts and to treat kidney diseases. |
| Druh dokumentu: | article in journal/newspaper |
| Jazyk: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/40980193; PUBMED: 40980193; PUBMEDCENTRAL: PMC12446076 |
| DOI: | 10.3389/ti.2025.14747 |
| Dostupnost: | https://inserm.hal.science/inserm-05357582 https://inserm.hal.science/inserm-05357582v1/document https://inserm.hal.science/inserm-05357582v1/file/ti-38-14747%20%281%29.pdf https://doi.org/10.3389/ti.2025.14747 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Přístupové číslo: | edsbas.3F89CB66 |
| Databáze: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | International audience ; Tubuloids have become a promising tool for modeling and regenerating kidney disease, although their ability for integration and regeneration in vivo is not well documented. Here, we established, characterized, and compared human tubuloids using two optimized protocols: one involving prior isolation of tubular cells (Crude tubuloids) and the other involving prior isolation of proximal tubular cells (F4 tubuloids). Next, healthy rat-derived tubuloids were established using this protocol. Finally, we compared two strategies for delivering GFP tubuloids to a kidney host: 1) subcapsular/intracortical injection and 2) tubuloid infusion during normothermic preservation in a rat transplantation model and a discarded human kidney. F4 tubuloids achieved a higher level of differentiation state compared to Crude tubuloids. When analyzing tubuloid delivery to the kidney, normothermic perfusion was found to be more efficient than in vivo injection. Moreover, fully developed tubules were observed in the host parenchyma at 1 week and 1 month after infusion during normothermic perfusion represent a potential strategy to enhance the translatability of kidney regenerative therapies into clinical practice to condition kidney grafts and to treat kidney diseases. |
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| DOI: | 10.3389/ti.2025.14747 |