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Simultaneous Detection of Morphine and Codeine in the Presence of Ascorbic Acid and Uric Acid and in Human Plasma at Nafion Single-Walled Carbon Nanotube Thin-Film Electrode

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Title: Simultaneous Detection of Morphine and Codeine in the Presence of Ascorbic Acid and Uric Acid and in Human Plasma at Nafion Single-Walled Carbon Nanotube Thin-Film Electrode
Authors: Wester, Niklas, Mynttinen, Elsi, Etula, Jarkko, Lilius, Tuomas, Kalso, Eija, Kauppinen, Esko I., Laurila, Tomi, Koskinen, Jari
Contributors: HUSLAB, Medicum, Department of Pharmacology, University of Helsinki, Department of Clinical Pharmacology, HUS Perioperative, Intensive Care and Pain Medicine, Eija Kalso / Principal Investigator, University Management, Department of Diagnostics and Therapeutics, Anestesiologian yksikkö
Publisher Information: American Chemical Society
Publication Year: 2019
Collection: Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto
Subject Terms: ELECTROCHEMICAL DETERMINATION, VOLTAMMETRIC OXIDATION, RAMAN-SPECTROSCOPY, PHARMACOKINETICS, METABOLITE, DRUGS, ACETAMINOPHEN, MEMBRANES, DOPAMINE, BEHAVIOR, Biomedicine, Chemical sciences
Description: In clinical settings, the dosing and differential diagnosis of the poisoning of morphine (MO) and codeine (CO) is challenging due to interindividual variations in metabolism. However, direct electrochemical detection of these analytes from biological matrices is inherently challenging due to interference from large concentrations of anions, such as ascorbic acid (AA) and uric acid (UA), as well as fouling of the electrode by proteins. In this work, a disposable Nafion-coated single-walled carbon nanotube network (SWCNT) electrode was developed. We show facile electron transfer and efficient charge separation between the interfering anions and positively charged MO and CO, as well as significantly reduced matrix effect in human plasma. The Nafion coating alters the voltammetric response of MO and CO, enabling simultaneous detection. With this SWCNT/Nafion electrode, two linear ranges of 0.05-1 and 1-10 mu M were found for MO and one linear range of 0.1-50 mu M for CO. Moreover, the selective and simultaneous detection of MO and CO was achieved in large excess of AA and UA, as well as, for the first time, in unprocessed human plasma. The favorable properties of this electrode enabled measurements in plasma with only mild dilution and without the precipitation of proteins. ; Peer reviewed
Document Type: article in journal/newspaper
File Description: application/pdf
Language: English
Relation: This work was supported by Business Finland (FEDOC project, grant number 211637 and FEPOD-TUTLI project, grant number 211773). Dr. Jenni Viinamaki, Department of Clinical Pharmacology, University of Helsinki, is acknowledged for expert assistance and providing plasma samples. The authors also acknowledge the provisions of facilities of Otanano.; http://hdl.handle.net/10138/307425; 000493751800012
Availability: http://hdl.handle.net/10138/307425
Rights: info:eu-repo/semantics/openAccess ; openAccess
Accession Number: edsbas.34FED96B
Database: BASE
Description
Abstract:In clinical settings, the dosing and differential diagnosis of the poisoning of morphine (MO) and codeine (CO) is challenging due to interindividual variations in metabolism. However, direct electrochemical detection of these analytes from biological matrices is inherently challenging due to interference from large concentrations of anions, such as ascorbic acid (AA) and uric acid (UA), as well as fouling of the electrode by proteins. In this work, a disposable Nafion-coated single-walled carbon nanotube network (SWCNT) electrode was developed. We show facile electron transfer and efficient charge separation between the interfering anions and positively charged MO and CO, as well as significantly reduced matrix effect in human plasma. The Nafion coating alters the voltammetric response of MO and CO, enabling simultaneous detection. With this SWCNT/Nafion electrode, two linear ranges of 0.05-1 and 1-10 mu M were found for MO and one linear range of 0.1-50 mu M for CO. Moreover, the selective and simultaneous detection of MO and CO was achieved in large excess of AA and UA, as well as, for the first time, in unprocessed human plasma. The favorable properties of this electrode enabled measurements in plasma with only mild dilution and without the precipitation of proteins. ; Peer reviewed