Standardised TruAI Automated Quantification of Intracellular Neuromelanin Granules in Human Brain Tissue Sections
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| Název: | Standardised TruAI Automated Quantification of Intracellular Neuromelanin Granules in Human Brain Tissue Sections |
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| Autoři: | Cuadros Arasa, Thais, Chatterton, Zac, Burke, Amy, Ansari Mahabadian, Anahid, Compte, Joan, Filimontseva, Anastasia, Vila, Miquel |
| Přispěvatelé: | Institut Català de la Salut, Filimontseva A, Chatterton Z, Burke A, Mahabadian AA The University of Sydney Brain and Mind Centre & Faculty of Medicine and Health, School of Medical Sciences, Sydney, NSW, Australia. Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, Maryland, USA. Cuadros T Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, Maryland, USA. Grup de Recerca de Malalties Neurodegeneratives, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Network Center for Biomedical Research in Neurodegenerative Diseases (CIBERNED), Barcelona, Spain. Compte J Grup de Recerca de Malalties Neurodegeneratives, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Network Center for Biomedical Research in Neurodegenerative Diseases (CIBERNED), Barcelona, Spain. Vila M Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, Maryland, USA. Grup de Recerca de Malalties Neurodegeneratives, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Network Center for Biomedical Research in Neurodegenerative Diseases (CIBERNED), Barcelona, Spain. Department of Biochemistry and Molecular Biology, Institute of Neuroscience, Autonomous University of Barcelona, Bellaterra, Spain. Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Zdroj: | Scientia |
| Informace o vydavateli: | Wiley |
| Rok vydání: | 2025 |
| Témata: | Neurones, Imatges - Processament, Cervell, Intel·ligència artificial, PHENOMENA AND PROCESSES::Mathematical Concepts::Algorithms::Artificial Intelligence, ANATOMY::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Cytoplasmic Granules, ANATOMY::Nervous System::Central Nervous System::Brain, INFORMATION SCIENCE::Information Science::Computing Methodologies::Image Processing, Computer-Assisted, CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Amino Acids::Amino Acids, Cyclic::Amino Acids, Aromatic::Tyrosine::Melanins, ANATOMY::Nervous System::Neurons, FENÓMENOS Y PROCESOS::conceptos matemáticos::algoritmos::inteligencia artificial, ANATOMÍA::células::estructuras celulares::espacio intracelular::citoplasma::estructuras citoplasmáticas::gránulos citoplasmáticos, ANATOMÍA::sistema nervioso::sistema nervioso central::encéfalo, CIENCIA DE LA INFORMACIÓN::Ciencias de la información::metodologías computacionales::procesamiento de imágenes asistido por ordenador, COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::aminoácidos::aminoácidos cíclicos::aminoácidos aromáticos::tirosina::melaninas, ANATOMÍA::sistema nervioso::neuronas |
| Popis: | Artificial intelligence automation; Colour identification; Computer‐assisted quantitation ; Automatización de la inteligencia artificial; Identificación de colores; Cuantificación asistida por ordenador ; Automatització de la intel·ligència artificial; Identificació de colors; Quantificació assistida per ordinador ; Aims To standardise and automate the quantitation of human-unique neuromelanin granules in catecholamine neurons in post-mortem tissue sections from healthy individuals at different ages to understand any changes in these granules with age. Methods Five- to 6-μm-thick fixed and paraffin-embedded transverse midbrain tissue sections were supplied from 47 cases from three brain banks following ethical approvals. Sections were prepared and automated digital images acquired. Standardisation and automation of the quantification of neuromelanin granules was performed using the TruAI feature of the Olympus VS200 desktop platform. Comparisons between stained and unstained sections as well as correlations with age were performed. Results The automated platform reliably identified both stained and unstained intracellular neuromelanin granules and extracellular pigments, showing high reproducibility in measurements across laboratories using different tissue processing methods. Extraneuronal pigments were significantly smaller than intracellular neuromelanin granules. Sections processed for haematoxylin and eosin staining impacted the size and colour of both neuromelanin and the neurons containing neuromelanin. Haematoxylin made neuromelanin bluer, and the increased tissue processing made the intracellular area occupied by neuromelanin smaller in younger people. There was an increase in neuromelanin optical density and colour change (browner) with age. Conclusions The TruAI automated platform reliably quantifies individual neuromelanin granules in catecholamine neurons. Extraneuronal pigments are considerably smaller in size than intracellular neuromelanin, and intracellular neuromelanin changes its properties ... |
| Druh dokumentu: | article in journal/newspaper |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| Relation: | Neuropathology and Applied Neurobiology;51(4); https://doi.org/10.1111/nan.70033; Filimontseva A, Cuadros T, Chatterton Z, Burke A, Mahabadian AA, Compte J, et al. Standardised TruAI Automated Quantification of Intracellular Neuromelanin Granules in Human Brain Tissue Sections. Neuropathol Appl Neurobiol. 2025 Aug;51(4):e70033.; https://hdl.handle.net/11351/13966; 001547581000001 |
| DOI: | 10.1111/nan.70033 |
| Dostupnost: | https://hdl.handle.net/11351/13966 https://doi.org/10.1111/nan.70033 |
| Rights: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess |
| Přístupové číslo: | edsbas.2F8D418B |
| Databáze: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | Artificial intelligence automation; Colour identification; Computer‐assisted quantitation ; Automatización de la inteligencia artificial; Identificación de colores; Cuantificación asistida por ordenador ; Automatització de la intel·ligència artificial; Identificació de colors; Quantificació assistida per ordinador ; Aims To standardise and automate the quantitation of human-unique neuromelanin granules in catecholamine neurons in post-mortem tissue sections from healthy individuals at different ages to understand any changes in these granules with age. Methods Five- to 6-μm-thick fixed and paraffin-embedded transverse midbrain tissue sections were supplied from 47 cases from three brain banks following ethical approvals. Sections were prepared and automated digital images acquired. Standardisation and automation of the quantification of neuromelanin granules was performed using the TruAI feature of the Olympus VS200 desktop platform. Comparisons between stained and unstained sections as well as correlations with age were performed. Results The automated platform reliably identified both stained and unstained intracellular neuromelanin granules and extracellular pigments, showing high reproducibility in measurements across laboratories using different tissue processing methods. Extraneuronal pigments were significantly smaller than intracellular neuromelanin granules. Sections processed for haematoxylin and eosin staining impacted the size and colour of both neuromelanin and the neurons containing neuromelanin. Haematoxylin made neuromelanin bluer, and the increased tissue processing made the intracellular area occupied by neuromelanin smaller in younger people. There was an increase in neuromelanin optical density and colour change (browner) with age. Conclusions The TruAI automated platform reliably quantifies individual neuromelanin granules in catecholamine neurons. Extraneuronal pigments are considerably smaller in size than intracellular neuromelanin, and intracellular neuromelanin changes its properties ... |
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| DOI: | 10.1111/nan.70033 |