Disc antenna enhanced infrared spectroscopy: From felf-assembled monolayers to membrane proteins
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| Titel: | Disc antenna enhanced infrared spectroscopy: From felf-assembled monolayers to membrane proteins |
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| Autoren: | Pfitzner, Emanuel, Seki, Hirofumi, Schlesinger, Ramona, Ataka, Kenichi, Heberle, Joachim |
| Publikationsjahr: | 2018 |
| Bestand: | FU Berlin: Refubium |
| Schlagwörter: | FTIR difference spectroscopy, membrane protein, nanostencil lithography, retinal, self-assembled monolayer, surface-enhanced infrared absorption spectroscopy, ddc:530 |
| Beschreibung: | Plasmonic surfaces have emerged as a powerful platform for biomolecular sensing applications and can be designed to optimize the plasmonic resonance for probing molecular vibrations at utmost sensitivity. Here, we present a facile procedure to generate metallic microdisc antenna arrays that are employed in surface-enhanced infrared absorption (SEIRA) spectroscopy of biomolecules. Transmission electron microscopy (TEM) grids are used as shadow mask deployed during physical vapor deposition of gold. The resulting disc-shaped antennas exhibit enhancement factors of the vibrational bands of 4 × 104 giving rise to a detection limit <1 femtomol (10–15 mol) of molecules. Surface-bound monolayers of 4-mercaptobenzoic acid show polyelectrolyte behavior when titrated with cations in the aqueous medium. Conformational rigidity of the self-assembled monolayer is validated by density functional theory calculations. The membrane protein sensory rhodopsin II is tethered to the disc antenna arrays and is fully functional as inferred from the light-induced SEIRA difference spectra. As an advance to previous studies, the accessible frequency range is improved and extended into the fingerprint region. |
| Publikationsart: | article in journal/newspaper |
| Dateibeschreibung: | 11 Seiten; application/pdf |
| Sprache: | English |
| DOI: | 10.17169/refubium-26164 |
| DOI: | 10.1021/acssensors.8b00139 |
| Verfügbarkeit: | https://refubium.fu-berlin.de/handle/fub188/26404 https://doi.org/10.17169/refubium-26164 https://doi.org/10.1021/acssensors.8b00139 |
| Rights: | http://www.fu-berlin.de/sites/refubium/rechtliches/Nutzungsbedingungen |
| Dokumentencode: | edsbas.26EEC99D |
| Datenbank: | BASE |
Nájsť tento článok vo Web of Science | Abstract: | Plasmonic surfaces have emerged as a powerful platform for biomolecular sensing applications and can be designed to optimize the plasmonic resonance for probing molecular vibrations at utmost sensitivity. Here, we present a facile procedure to generate metallic microdisc antenna arrays that are employed in surface-enhanced infrared absorption (SEIRA) spectroscopy of biomolecules. Transmission electron microscopy (TEM) grids are used as shadow mask deployed during physical vapor deposition of gold. The resulting disc-shaped antennas exhibit enhancement factors of the vibrational bands of 4 × 104 giving rise to a detection limit <1 femtomol (10–15 mol) of molecules. Surface-bound monolayers of 4-mercaptobenzoic acid show polyelectrolyte behavior when titrated with cations in the aqueous medium. Conformational rigidity of the self-assembled monolayer is validated by density functional theory calculations. The membrane protein sensory rhodopsin II is tethered to the disc antenna arrays and is fully functional as inferred from the light-induced SEIRA difference spectra. As an advance to previous studies, the accessible frequency range is improved and extended into the fingerprint region. |
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| DOI: | 10.17169/refubium-26164 |