Development of a low-cost robotized 3D-prototype for automated optical microscopy diagnosis: An open-source system
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| Název: | Development of a low-cost robotized 3D-prototype for automated optical microscopy diagnosis: An open-source system |
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| Autoři: | Motta de Carvalho, Bruno, JOSEPH, JOAN, DANTAS DE OLIVEIRA, ALLISSON, Rubio Maturana, Carles, Zarzuela Serrat, Francesc, Sulleiro, Elena, Prats, Clara |
| Přispěvatelé: | Institut Català de la Salut, Dantas de OliveiraI A Computational Biology and Complex Systems Group, Physics Department, Universitat Politècnica de Catalunya (UPC), Castelldefels, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Rubio MaturanaI C Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Microbiologia i Genètica, Universitat Autònoma de Barcelona, Bellaterra, Spain. Zarzuela Serrat F, Joseph-Munné J Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Motta Carvalho B Department of Informatics and Applied Mathematics, Federal University of Rio Grande do Norte, Natal, Brazil. Sulleiro E Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Microbiologia i Genètica, Universitat Autònoma de Barcelona, Bellaterra, Spain. CIBERINFEC, ISCIII- CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain. Prats C Computational Biology and Complex Systems Group, Physics Department, Universitat Politècnica de Catalunya (UPC), Castelldefels, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Zdroj: | Scientia |
| Informace o vydavateli: | Public Library Science |
| Rok vydání: | 2024 |
| Témata: | Microscòpia, Impressió 3D, Robòtica, Automatització, TECHNOLOGY, INDUSTRY, AND AGRICULTURE::Technology, and Agriculture::Technology::Automation, INFORMATION SCIENCE::Information Science::Computing Methodologies::Computer Graphics::Computer-Aided Design::Information Science::Printing, Three-Dimensional, INFORMATION SCIENCE::Information Science::Computing Methodologies::Algorithms::Artificial Intelligence::Robotics, ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Microscopy, Other subheadings::Other subheadings::/instrumentation, TECNOLOGÍA, INDUSTRIA Y AGRICULTURA::tecnología, industria y agricultura::tecnología::automatización, CIENCIA DE LA INFORMACIÓN::Ciencias de la información::metodologías computacionales::gráficos por ordenador::diseño asistido por ordenador::Ciencias de la información::impresión tridimensional, CIENCIA DE LA INFORMACIÓN::Ciencias de la información::metodologías computacionales::algoritmos::inteligencia artificial::robótica, TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::diagnóstico por imagen::microscopía, Otros calificadores::Otros calificadores::/instrumentación |
| Popis: | Robotized 3D-prototype; Optical microscopy; Open-source ; Prototipo 3D robotizado; Microscopía óptica; Código abierto ; Prototip 3D robotitzat; Microscòpia òptica; Codi obert ; In a clinical context, conventional optical microscopy is commonly used for the visualization of biological samples for diagnosis. However, the availability of molecular techniques and rapid diagnostic tests are reducing the use of conventional microscopy, and consequently the number of experienced professionals starts to decrease. Moreover, the continuous visualization during long periods of time through an optical microscope could affect the final diagnosis results due to induced human errors and fatigue. Therefore, microscopy automation is a challenge to be achieved and address this problem. The aim of the study is to develop a low-cost automated system for the visualization of microbiological/parasitological samples by using a conventional optical microscope, and specially designed for its implementation in resource-poor settings laboratories. A 3D-prototype to automate the majority of conventional optical microscopes was designed. Pieces were built with 3D-printing technology and polylactic acid biodegradable material with Tinkercad/Ultimaker Cura 5.1 slicing softwares. The system’s components were divided into three subgroups: microscope stage pieces, storage/autofocus-pieces, and smartphone pieces. The prototype is based on servo motors, controlled by Arduino open-source electronic platform, to emulate the X-Y and auto-focus (Z) movements of the microscope. An average time of 27.00 ± 2.58 seconds is required to auto-focus a single FoV. Auto-focus evaluation demonstrates a mean average maximum Laplacian value of 11.83 with tested images. The whole automation process is controlled by a smartphone device, which is responsible for acquiring images for further diagnosis via convolutional neural networks. The prototype is specially designed for resource-poor settings, where microscopy diagnosis is still a routine process. The coalescence ... |
| Druh dokumentu: | article in journal/newspaper |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| Relation: | PLOS ONE;19(6); https://doi.org/10.1371/journal.pone.0304085; https://hdl.handle.net/11351/11645 |
| DOI: | 10.1371/journal.pone.0304085 |
| Dostupnost: | https://hdl.handle.net/11351/11645 https://doi.org/10.1371/journal.pone.0304085 |
| Rights: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess |
| Přístupové číslo: | edsbas.25E7FD05 |
| Databáze: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | Robotized 3D-prototype; Optical microscopy; Open-source ; Prototipo 3D robotizado; Microscopía óptica; Código abierto ; Prototip 3D robotitzat; Microscòpia òptica; Codi obert ; In a clinical context, conventional optical microscopy is commonly used for the visualization of biological samples for diagnosis. However, the availability of molecular techniques and rapid diagnostic tests are reducing the use of conventional microscopy, and consequently the number of experienced professionals starts to decrease. Moreover, the continuous visualization during long periods of time through an optical microscope could affect the final diagnosis results due to induced human errors and fatigue. Therefore, microscopy automation is a challenge to be achieved and address this problem. The aim of the study is to develop a low-cost automated system for the visualization of microbiological/parasitological samples by using a conventional optical microscope, and specially designed for its implementation in resource-poor settings laboratories. A 3D-prototype to automate the majority of conventional optical microscopes was designed. Pieces were built with 3D-printing technology and polylactic acid biodegradable material with Tinkercad/Ultimaker Cura 5.1 slicing softwares. The system’s components were divided into three subgroups: microscope stage pieces, storage/autofocus-pieces, and smartphone pieces. The prototype is based on servo motors, controlled by Arduino open-source electronic platform, to emulate the X-Y and auto-focus (Z) movements of the microscope. An average time of 27.00 ± 2.58 seconds is required to auto-focus a single FoV. Auto-focus evaluation demonstrates a mean average maximum Laplacian value of 11.83 with tested images. The whole automation process is controlled by a smartphone device, which is responsible for acquiring images for further diagnosis via convolutional neural networks. The prototype is specially designed for resource-poor settings, where microscopy diagnosis is still a routine process. The coalescence ... |
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| DOI: | 10.1371/journal.pone.0304085 |