Ruthenium Antivenom Inhibits the Defibrinogenating Activity of Crotalus adamanteus Venom in Rabbits
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| Název: | Ruthenium Antivenom Inhibits the Defibrinogenating Activity of Crotalus adamanteus Venom in Rabbits |
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| Autoři: | Vance G. Nielsen |
| Zdroj: | International Journal of Molecular Sciences, Vol 25, Iss 12, p 6334 (2024) |
| Informace o vydavateli: | MDPI AG |
| Rok vydání: | 2024 |
| Sbírka: | Directory of Open Access Journals: DOAJ Articles |
| Témata: | snake venom thrombin-like enzyme, thrombelastography, rabbit, platelet inhibition, ruthenium, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| Popis: | Eastern Diamondback Rattlesnake ( Crotalus adamanteus ) envenomation is a medical emergency encountered in the Southeastern United States. The venom contains a snake venom thrombin-like enzyme (SVTLE) that is defibrinogenating, causing coagulopathy without effects on platelets in humans. This investigation utilized thrombelastographic methods to document this coagulopathy kinetically on the molecular level in a rabbit model of envenomation via the analyses of whole blood samples without and with platelet inhibition. Subsequently, the administration of a novel ruthenium compound containing site-directed antivenom abrogated the coagulopathic effects of envenomation in whole blood without platelet inhibition and significantly diminished loss of coagulation in platelet-inhibited samples. This investigation provides coagulation kinetic insights into the molecular interactions and results of SVTLE on fibrinogen-dependent coagulation and confirmation of the efficacy of a ruthenium antivenom. These results serve as a rationale to investigate the coagulopathic effects of other venoms with this model and assess the efficacy of this site-directed antivenom. |
| Druh dokumentu: | article in journal/newspaper |
| Jazyk: | English |
| Relation: | https://www.mdpi.com/1422-0067/25/12/6334; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/01dddb4e27b244ce9c92f5b71de7c6a6 |
| DOI: | 10.3390/ijms25126334 |
| Dostupnost: | https://doi.org/10.3390/ijms25126334 https://doaj.org/article/01dddb4e27b244ce9c92f5b71de7c6a6 |
| Přístupové číslo: | edsbas.2455A41F |
| Databáze: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | Eastern Diamondback Rattlesnake ( Crotalus adamanteus ) envenomation is a medical emergency encountered in the Southeastern United States. The venom contains a snake venom thrombin-like enzyme (SVTLE) that is defibrinogenating, causing coagulopathy without effects on platelets in humans. This investigation utilized thrombelastographic methods to document this coagulopathy kinetically on the molecular level in a rabbit model of envenomation via the analyses of whole blood samples without and with platelet inhibition. Subsequently, the administration of a novel ruthenium compound containing site-directed antivenom abrogated the coagulopathic effects of envenomation in whole blood without platelet inhibition and significantly diminished loss of coagulation in platelet-inhibited samples. This investigation provides coagulation kinetic insights into the molecular interactions and results of SVTLE on fibrinogen-dependent coagulation and confirmation of the efficacy of a ruthenium antivenom. These results serve as a rationale to investigate the coagulopathic effects of other venoms with this model and assess the efficacy of this site-directed antivenom. |
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| DOI: | 10.3390/ijms25126334 |