Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes
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| Název: | Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes |
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| Přispěvatelé: | June Seok Heo, Sinyoung Kim, Kim, Sin Young |
| Informace o vydavateli: | Nature Publishing Group |
| Rok vydání: | 2022 |
| Témata: | Adipose Tissue / metabolism, Exosomes / metabolism, Human Umbilical Vein Endothelial Cells / metabolism, Humans, Inflammation / metabolism, Mesenchymal Stem Cells / metabolism, Neovascularization, Physiologic |
| Popis: | Stem cell-derived exosomes are efficient and safe therapeutic tools for transferring endogenous biological cargo or functional biomolecules for regenerative medicine. The regulation of inflammation and angiogenesis plays a pivotal role in wound healing and tissue regeneration. The purpose of this study was to investigate the anti-inflammatory and pro-angiogenic roles of human adipose mesenchymal stem cell-derived exosomes, focusing on the underlying mechanisms. Exosomes inhibited LPS-induced inflammation by activating ROCK1 and PTEN expression. Moreover, microRNAs (miR-132 and miR-146a) released from exosomes upregulated the expression of pro-angiogenic genes and promoted proliferation activity and tube formation in human umbilical vein endothelial cells. Exosomal effects were verified using ROCK1/PTEN inhibitors for anti-inflammation and miR-132/miR-146a inhibitors for pro-angiogenesis. Our findings suggest that exosomes exert anti-inflammatory effects by targeting the ROCK1/PTEN pathway and exhibit pro-angiogenic effects via delivery of miR-132 and miR-146a. Taken together, these results suggest that exosomes may be promising therapeutic candidates for curing diseases involved in inflammation and angiogenesis. ; open |
| Druh dokumentu: | article in journal/newspaper |
| Jazyk: | English |
| Relation: | SCIENTIFIC REPORTS; J02646; https://ir.ymlib.yonsei.ac.kr/handle/22282913/188741; T202201794 |
| DOI: | 10.1038/s41598-022-06824-1 |
| Dostupnost: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/188741 https://doi.org/10.1038/s41598-022-06824-1 |
| Rights: | CC BY-NC-ND 2.0 KR |
| Přístupové číslo: | edsbas.1B75AD11 |
| Databáze: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | Stem cell-derived exosomes are efficient and safe therapeutic tools for transferring endogenous biological cargo or functional biomolecules for regenerative medicine. The regulation of inflammation and angiogenesis plays a pivotal role in wound healing and tissue regeneration. The purpose of this study was to investigate the anti-inflammatory and pro-angiogenic roles of human adipose mesenchymal stem cell-derived exosomes, focusing on the underlying mechanisms. Exosomes inhibited LPS-induced inflammation by activating ROCK1 and PTEN expression. Moreover, microRNAs (miR-132 and miR-146a) released from exosomes upregulated the expression of pro-angiogenic genes and promoted proliferation activity and tube formation in human umbilical vein endothelial cells. Exosomal effects were verified using ROCK1/PTEN inhibitors for anti-inflammation and miR-132/miR-146a inhibitors for pro-angiogenesis. Our findings suggest that exosomes exert anti-inflammatory effects by targeting the ROCK1/PTEN pathway and exhibit pro-angiogenic effects via delivery of miR-132 and miR-146a. Taken together, these results suggest that exosomes may be promising therapeutic candidates for curing diseases involved in inflammation and angiogenesis. ; open |
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| DOI: | 10.1038/s41598-022-06824-1 |