Primary data for BJC Carbonic anhydrase IX is a pH-stat that sets an acidic tumour extracellular pH in vivo
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| Název: | Primary data for BJC Carbonic anhydrase IX is a pH-stat that sets an acidic tumour extracellular pH in vivo |
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| Autoři: | Griffiths, JR, Lee, Shen-Han, McIntyre, Dominick John, Honess, Davina Jean, Hulikova, Alzbeta, Pacheco-Torres, Jesus, Cerdan, Sebastian, Swietach, Pawel, Harris, Adrian L |
| Rok vydání: | 2023 |
| Sbírka: | Apollo - University of Cambridge Repository |
| Témata: | Carbonic anhydrase IX, Extracellular pH, Intracellular pH, ISUCA, Magnetic Resonance Spectroscopy, MRS, pH-stat, Tumour pH |
| Popis: | The paper describing these data is available free of charge from https://www.nature.com/articles/s41416-018-0216-5 and readers are encouraged to download it to view the figures and full details of acquisition protocols and analysis of the data presented here. Figure 6 sources.zip Figure 6 plots intracellular pH (pHi) for each tumour measured by magnetic resonance spectroscopy (MRS), lactate overall measured by enzymatic methods, and models of intra/extracellular lactate distribution. Figure 6 as png and svg files (lac_combo2.png/svg) phi_vs_phe.csv: Experimentally measured pHi (31P MRS) and extracellular pH (pHe) values (1H MRS of ISUCA) in empty vector control EV5 and carbonic anhydrase 9 overexpressing CA9 model tumours in mice CA9_lactate_enzymatic files - enzymatically measured lactate concentrations for tumour samples. lactateplot.py: python code to generate the plots in Figure 6, including computing intracellular & extracellular lactate from measured total lactate, pHi and pHe, and testing normality and significance of tumour type for pHi (ns) and pHe (p0.001 Mann-Whitney test). Statistical output in laccalc_output.xlsx Figure 5 sources.zip Figure 5 compares pHi and pHe values for CA9 tumour measured by 1H magnetic resonance spectroscopy of ISUCA, demonstrating minimal overlap phi_vs_phe.csv: Experimentally measured pHi (31P MRS) and pHe values (1H MRS of ISUCA) in EV and CA9 tumours. EV data not plotted. phi_vs_phe.csv: Experimentally measured pHi (31P MRS) and pHe values (1H MRS of ISUCA) in EV and CAIX/1 tumours phi_vs_e_ca9.py: Code to generate pHi and pHe box and whisker plots from above data Figure in multiple graphic formats raw data for plotting Fig 2.xlsx Figure 2 contains plots of exofacial CA and CO2 hydration rates for the model tumours in this study; the raw data are presented in this spreadsheet. Code for 1H MRS analysis.zip This contains a walkthrough for the spectroscopic quantitation of the 1H MRS data provided in 1H MRS data.zip, together with the python 2 code used to generate the pH ... |
| Druh dokumentu: | dataset |
| Popis souboru: | jMRUI http://www.jmrui.eu/ python www.python.org Excel; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet; application/octet-stream |
| Jazyk: | unknown |
| Relation: | https://www.repository.cam.ac.uk/handle/1810/361916; https://doi.org/10.17863/CAM.26029 |
| DOI: | 10.17863/CAM.26029 |
| Dostupnost: | https://www.repository.cam.ac.uk/handle/1810/361916 https://doi.org/10.17863/CAM.26029 |
| Rights: | Attribution 4.0 International (CC BY 4.0) ; https://creativecommons.org/licenses/by/4.0/ |
| Přístupové číslo: | edsbas.136C8457 |
| Databáze: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | The paper describing these data is available free of charge from https://www.nature.com/articles/s41416-018-0216-5 and readers are encouraged to download it to view the figures and full details of acquisition protocols and analysis of the data presented here. Figure 6 sources.zip Figure 6 plots intracellular pH (pHi) for each tumour measured by magnetic resonance spectroscopy (MRS), lactate overall measured by enzymatic methods, and models of intra/extracellular lactate distribution. Figure 6 as png and svg files (lac_combo2.png/svg) phi_vs_phe.csv: Experimentally measured pHi (31P MRS) and extracellular pH (pHe) values (1H MRS of ISUCA) in empty vector control EV5 and carbonic anhydrase 9 overexpressing CA9 model tumours in mice CA9_lactate_enzymatic files - enzymatically measured lactate concentrations for tumour samples. lactateplot.py: python code to generate the plots in Figure 6, including computing intracellular & extracellular lactate from measured total lactate, pHi and pHe, and testing normality and significance of tumour type for pHi (ns) and pHe (p0.001 Mann-Whitney test). Statistical output in laccalc_output.xlsx Figure 5 sources.zip Figure 5 compares pHi and pHe values for CA9 tumour measured by 1H magnetic resonance spectroscopy of ISUCA, demonstrating minimal overlap phi_vs_phe.csv: Experimentally measured pHi (31P MRS) and pHe values (1H MRS of ISUCA) in EV and CA9 tumours. EV data not plotted. phi_vs_phe.csv: Experimentally measured pHi (31P MRS) and pHe values (1H MRS of ISUCA) in EV and CAIX/1 tumours phi_vs_e_ca9.py: Code to generate pHi and pHe box and whisker plots from above data Figure in multiple graphic formats raw data for plotting Fig 2.xlsx Figure 2 contains plots of exofacial CA and CO2 hydration rates for the model tumours in this study; the raw data are presented in this spreadsheet. Code for 1H MRS analysis.zip This contains a walkthrough for the spectroscopic quantitation of the 1H MRS data provided in 1H MRS data.zip, together with the python 2 code used to generate the pH ... |
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| DOI: | 10.17863/CAM.26029 |