A Comprehensive Study of Solvent Effects on Dipole Moment, Quenching, and Lifetime in Fluorescent Dyes
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| Title: | A Comprehensive Study of Solvent Effects on Dipole Moment, Quenching, and Lifetime in Fluorescent Dyes |
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| Authors: | Manjula Katageri |
| Source: | International Journal of Intelligent Systems and Applications in Engineering; Vol. 12 No. 23s (2024); 2382–2390 |
| Publisher Information: | International Journal of Intelligent Systems and Applications in Engineering, 2024. |
| Publication Year: | 2024 |
| Subject Terms: | fluorescent dyes, fluorescence spectroscopy, UV-Vis absorption spectroscopy, non-polar solvents, time-correlated single-photon counting |
| Description: | This study examines the influence of solvent polarity and hydrogen bonding on the dipole moment, fluorescence quenching, and fluorescence lifetime of specific fluorescent dyes. We assessed fluorescence intensity and lifetime utilizing a variety of solvents—encompassing non-polar (hexane, toluene), polar aprotic (acetonitrile, DMSO), and polar protic (water, methanol, ethanol, isopropanol)—through UV-Vis absorption spectroscopy, fluorescence spectroscopy, and time-correlated single-photon counting (TCSPC). Our findings indicate that non-polar solvents augment fluorescence intensity and prolong fluorescence lifespan, whereas polar solvents markedly diminish both due to heightened quenching and non-radiative decay. Protic solvents, especially water, have the most pronounced quenching effects owing to hydrogen bonding interactions. The results correspond with the Lippert-Mataga connection, demonstrating that solvent polarity directly influences the excited-state characteristics of fluorescent dyes. This study offers critical insights for enhancing fluorescent dyes applicable in bioimaging, chemical sensing, and materials science. |
| Document Type: | Article |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 2147-6799 |
| Access URL: | https://www.ijisae.org/index.php/IJISAE/article/view/7347 |
| Rights: | CC BY SA |
| Accession Number: | edsair.issn21476799..60aecbf2ece5fd9746fc501d126ad28c |
| Database: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstract: | This study examines the influence of solvent polarity and hydrogen bonding on the dipole moment, fluorescence quenching, and fluorescence lifetime of specific fluorescent dyes. We assessed fluorescence intensity and lifetime utilizing a variety of solvents—encompassing non-polar (hexane, toluene), polar aprotic (acetonitrile, DMSO), and polar protic (water, methanol, ethanol, isopropanol)—through UV-Vis absorption spectroscopy, fluorescence spectroscopy, and time-correlated single-photon counting (TCSPC). Our findings indicate that non-polar solvents augment fluorescence intensity and prolong fluorescence lifespan, whereas polar solvents markedly diminish both due to heightened quenching and non-radiative decay. Protic solvents, especially water, have the most pronounced quenching effects owing to hydrogen bonding interactions. The results correspond with the Lippert-Mataga connection, demonstrating that solvent polarity directly influences the excited-state characteristics of fluorescent dyes. This study offers critical insights for enhancing fluorescent dyes applicable in bioimaging, chemical sensing, and materials science. |
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| ISSN: | 21476799 |