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Determination of oxidative stress level on four Mediterranean forest species

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Titel: Determination of oxidative stress level on four Mediterranean forest species
Autoren: Jakovljević, Tamara, Potočić, Nenad, Seletković, Ivan, Butorac, Lukrecija, Limić, Ivan, Jurinjak Tušek, Ana, Radojčić Redovniković, Ivana, Lovreškov, Lucija
Verlagsinformationen: 2025.
Publikationsjahr: 2025
Schlagwörter: nutrient concentration, Pinus spp, Quercus spp, oxidative stress, chlorophyll, defoliated trees, hydrogen peroxide, lipid peroxidation, antioxidative enzymes, undefoliated trees
Beschreibung: Mediterranean forest ecosystems in Croatia are of very high significance because of the ecological functions they provide. This region is highly sensitive to abiotic stresses such as air pollution, high sunlight, and high temperatures alongside dry periods. Therefore, it is important to monitor the state of these forest ecosystems and how they respond to these stresses. The study was conducted on the four most important forest species in the Mediterranean region along Adriatic coast of Croatia: pubescent oak (Quercus ubescens Willd.), holm oak (Quercus ilex L.), Aleppo pine (Pinus halepensis Mill.) and black pine (Pinus nigra J. F. Arnold.). Trees were selected and divided into two groups: trees with defoliation of >25% (defoliated) and trees with defoliation of 25% (undefoliated). Leaves and needles were collected from selected trees. Chlorophyll content, hydrogen peroxide content, lipid peroxidation and enzyme activity (superoxide dismutase, catalase, ascorbate peroxidase, non-specific peroxidase) were analyzed. Differences in oxidative stress indicators between defoliated trees and undefoliated trees were tested. Using a Principal Component Analysis (PCA) we investigated whether the oxidative stress indicators were associated with defoliation and environmental variables to determine if they could be used as early indicators of forest tree health. The results showed that there were significant differences for all species between the defoliated and undefoliated trees for at least one of the examined parameters. A principal component analysis showed that the enzyme ascorbate peroxidase can be an indicator of oxidative stress caused by ozone. By using oxidative stress indicators, it is possible to determine whether the trees are under stress even before visual damage occurs
Publikationsart: Conference object
Dokumentencode: edsair.dris...01492..6b245cf3495f4713de3e3eb3a4fc1a66
Datenbank: OpenAIRE
Beschreibung
Abstract:Mediterranean forest ecosystems in Croatia are of very high significance because of the ecological functions they provide. This region is highly sensitive to abiotic stresses such as air pollution, high sunlight, and high temperatures alongside dry periods. Therefore, it is important to monitor the state of these forest ecosystems and how they respond to these stresses. The study was conducted on the four most important forest species in the Mediterranean region along Adriatic coast of Croatia: pubescent oak (Quercus ubescens Willd.), holm oak (Quercus ilex L.), Aleppo pine (Pinus halepensis Mill.) and black pine (Pinus nigra J. F. Arnold.). Trees were selected and divided into two groups: trees with defoliation of >25% (defoliated) and trees with defoliation of 25% (undefoliated). Leaves and needles were collected from selected trees. Chlorophyll content, hydrogen peroxide content, lipid peroxidation and enzyme activity (superoxide dismutase, catalase, ascorbate peroxidase, non-specific peroxidase) were analyzed. Differences in oxidative stress indicators between defoliated trees and undefoliated trees were tested. Using a Principal Component Analysis (PCA) we investigated whether the oxidative stress indicators were associated with defoliation and environmental variables to determine if they could be used as early indicators of forest tree health. The results showed that there were significant differences for all species between the defoliated and undefoliated trees for at least one of the examined parameters. A principal component analysis showed that the enzyme ascorbate peroxidase can be an indicator of oxidative stress caused by ozone. By using oxidative stress indicators, it is possible to determine whether the trees are under stress even before visual damage occurs