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Removal of PSA from NCAM affects the survival of magnocellular vasopressin‐ and oxytocin‐producing neurons in organotypic cultures of the paraventricular nucleus

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Title: Removal of PSA from NCAM affects the survival of magnocellular vasopressin‐ and oxytocin‐producing neurons in organotypic cultures of the paraventricular nucleus
Authors: Vutskits, Laszlo, Gascon, Eduardo, Kiss, Jozsef Zoltan
Source: European Journal of Neuroscience, Vol. 17, No 10 (2003) pp. 2119-2126
Publisher Information: Wiley, 2003.
Publication Year: 2003
Subject Terms: Male, 0301 basic medicine, 616.8, Glycoside Hydrolases, Paraventricular Hypothalamic Nucleus/ cytology, Cell Survival, Oxytocin/biosynthesis, Neural Cell Adhesion Molecule L1, Oxytocin, Leukemia Inhibitory Factor, Rats, Sprague-Dawley, Neural Cell Adhesion Molecules/ metabolism, 03 medical and health sciences, Organ Culture Techniques, Animals, Ciliary Neurotrophic Factor, Neural Cell Adhesion Molecules, Cell Survival/physiology, Neurons, Lymphokines, 0303 health sciences, Neuronal Plasticity, Interleukin-6, Ciliary Neurotrophic Factor/pharmacology, Membrane Proteins, Vasopressins/biosynthesis, Lymphokines/pharmacology, Neuronal Plasticity/physiology, Neurons/ cytology/drug effects/ metabolism, Neural Cell Adhesion Molecule L1/ metabolism, Growth Inhibitors, ddc:616.8, Rats, Glycoside Hydrolases/pharmacology, Sialic Acids/ metabolism, Sialic Acids, Membrane Proteins/metabolism, Growth Inhibitors/pharmacology, Paraventricular Hypothalamic Nucleus
Description: The expression of the polysialic acid neural cell adhesion molecule (PSA‐NCAM) in the hypothalamo‐neurohypophyseal system has been correlated with morphofunctional plasticity. In this study, we investigated the role of PSA‐NCAM in the survival of oxytocin (OT)‐ and vasopressin (VP)‐producing magnocellular cells of this system. We used a recently developed organotypic slice culture model of the rat hypothalamic paraventricular nucleus (PVN) in which ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) are potent survival factors for magnocellular neurons. We demonstrate by means of confocal microscopy that cultured magnocellular VP and OT neurons express strong immunoreactivity for PSA‐NCAM. Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons in the presence of low concentrations of CNTF. Endo N treatment did not change cell survival in the presence of LIF. These results suggest that, in addition to its role in neuro‐glial plasticity, PSA‐NCAM might also influence the trophic factor responsiveness of hypothalamic VP and OT neurosecretory cells.
Document Type: Article
File Description: application/pdf
Language: English
ISSN: 1460-9568
0953-816X
DOI: 10.1046/j.1460-9568.2003.02660.x
Access URL: https://pubmed.ncbi.nlm.nih.gov/12786978
https://core.ac.uk/display/60786724
https://archive-ouverte.unige.ch/unige:10506
https://www.ncbi.nlm.nih.gov/pubmed/12786978
https://europepmc.org/article/MED/12786978
https://archive-ouverte.unige.ch/unige:10506
Rights: Wiley Online Library User Agreement
Accession Number: edsair.doi.dedup.....fec7d9ec69d22f54eeb0be5ce9f4fb3b
Database: OpenAIRE
Description
Abstract:The expression of the polysialic acid neural cell adhesion molecule (PSA‐NCAM) in the hypothalamo‐neurohypophyseal system has been correlated with morphofunctional plasticity. In this study, we investigated the role of PSA‐NCAM in the survival of oxytocin (OT)‐ and vasopressin (VP)‐producing magnocellular cells of this system. We used a recently developed organotypic slice culture model of the rat hypothalamic paraventricular nucleus (PVN) in which ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) are potent survival factors for magnocellular neurons. We demonstrate by means of confocal microscopy that cultured magnocellular VP and OT neurons express strong immunoreactivity for PSA‐NCAM. Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons in the presence of low concentrations of CNTF. Endo N treatment did not change cell survival in the presence of LIF. These results suggest that, in addition to its role in neuro‐glial plasticity, PSA‐NCAM might also influence the trophic factor responsiveness of hypothalamic VP and OT neurosecretory cells.
ISSN:14609568
0953816X
DOI:10.1046/j.1460-9568.2003.02660.x