Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis
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| Title: | Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis |
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| Authors: | Jong Bok Yoon, Eung Sik Kong, Jin Sil Seong, Young Mee Park, Eun Mi Park, Yun Jeong Kim, Eui Chul Park, Kyoung-Soo Choi |
| Contributors: | Eui‐Chul Park, Jong‐Bok Yoon, Jin‐Sil Seong, Kyoung‐Soo Choi, Eung‐Sik Kong, Yun‐Jeong Kim, Young‐Mee Park, Eun‐Mi Park, Seong, Jin Sil |
| Source: | Preparative Biochemistry and Biotechnology. 36:19-35 |
| Publisher Information: | Informa UK Limited, 2006. |
| Publication Year: | 2006 |
| Subject Terms: | Electrophoresis, Liver/metabolism, Male, Proteomics, 0301 basic medicine, Spectrometry, Mass, Electrospray Ionization, Proteome, Thiobarbituric Acid Reactive Substances/metabolism, Thiobarbituric Acid Reactive Substances, Up-Regulation/radiation effects, Rats, Sprague-Dawley, 03 medical and health sciences, Reactive Oxygen Species/metabolism, Matrix-Assisted Laser Desorption-Ionization, Animals, Electrophoresis, Gel, Two-Dimensional, Proteins/analysis, Gel, 0303 health sciences, Spectrometry, Liver/radiation effects, Electrospray Ionization, Proteins, Proteomics/methods, Mass, Glutathione, Glutathione/metabolism, Rats, Up-Regulation, Lipid Peroxidation/radiation effects, Proteome/analysis, Liver, Gamma Rays, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Two-Dimensional, Sprague-Dawley, Lipid Peroxidation, Reactive Oxygen Species |
| Description: | Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation. |
| Document Type: | Article |
| File Description: | 19~35 |
| Language: | English |
| ISSN: | 1532-2297 1082-6068 |
| DOI: | 10.1080/10826060500388470 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/16428137 https://www.tandfonline.com/doi/abs/10.1080/10826060500388470 https://yonsei.pure.elsevier.com/en/publications/effect-of-ionizing-radiation-on-rat-tissue-proteomic-and-biochemi https://mdanderson.elsevierpure.com/en/publications/effect-of-ionizing-radiation-on-rat-tissue-proteomic-and-biochemi https://europepmc.org/article/MED/16428137 https://www.ncbi.nlm.nih.gov/pubmed/16428137 |
| Rights: | CC BY NC ND |
| Accession Number: | edsair.doi.dedup.....f7c2140bcf4439375ea9a4987dc64d6b |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation. |
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| ISSN: | 15322297 10826068 |
| DOI: | 10.1080/10826060500388470 |