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Detection of dicistroviruses RNA in blood of febrile Tanzanian children

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Název: Detection of dicistroviruses RNA in blood of febrile Tanzanian children
Autoři: Florian Laubscher, Zainab Mbarack, Jürgen May, Mary-Anne Hartley, Samuel Cordey, Tarsis Mlaganile, Mylène Docquier, Valérie D'Acremont, Noémie Boillat-Blanco, Josephine Samaka, Thomas Junier, Gael Vieille, Frank Kagoro, John Lusingu, Daniel Eibach, Caroline Tapparel, Cassandra Aldrich, Peter Sothmann, Kristina Keitel, Laurent Kaiser, Francisco J. Pérez-Rodriguez, Francisco Brito
Zdroj: Emerging Microbes & Infections
Emerg Microbes Infect
Emerging Microbes and Infections, Vol 8, Iss 1, Pp 613-623 (2019)
Emerging Microbes & Infections, Vol. 8, No 1 (2019) pp. 613-623
Emerging microbes & infections, vol. 8, no. 1, pp. 613-623
Cordey, Samuel; Laubscher, Florian; Hartley, Mary-Anne; Junier, Thomas; Pérez-Rodriguez, Francisco J; Keitel, Kristina; Vieille, Gael; Samaka, Josephine; Mlaganile, Tarsis; Kagoro, Frank; Boillat-Blanco, Noémie; Mbarack, Zainab; Docquier, Mylène; Brito, Francisco; Eibach, Daniel; May, Jürgen; Sothmann, Peter; Aldrich, Cassandra; Lusingu, John; Tapparel, Caroline; ... (2019). Detection of dicistroviruses RNA in blood of febrile Tanzanian children. Emerging microbes & infections, 8(1), pp. 613-623. Taylor & Francis 10.1080/22221751.2019.1603791 <http://dx.doi.org/10.1080/22221751.2019.1603791>
Informace o vydavateli: Informa UK Limited, 2019.
Rok vydání: 2019
Témata: 576.5, Male, 0301 basic medicine, de novo analysis, Fever, Epidemiology, Immunology, 610 Medicine & health, Virus Diseases/blood/genetics/virology, Infectious and parasitic diseases, RC109-216, Viral/blood, sera, Tanzanian children, Polymerase Chain Reaction, Tanzania, Microbiology, Article, Cohort Studies, 03 medical and health sciences, ddc:590, Virology, Drug Discovery, Humans, ddc:576.5, Preschool, Child, Phylogeny, ddc:616, 0303 health sciences, viremia, Infant, Fever/blood/virology, General Medicine, Dicistrovirus, QR1-502, 3. Good health, Infectious Diseases, Viruses/classification/isolation & purification, Virus Diseases, Child, Preschool, Viruses, RNA, RNA, Viral, Female, Parasitology, Fever/blood, Fever/virology, RNA, Viral/blood, Virus Diseases/blood, Virus Diseases/genetics, Virus Diseases/virology, Viruses/classification, Viruses/isolation & purification
Popis: Fever is the leading cause of paediatric outpatient consultations in Sub-Saharan Africa. Although most are suspected to be of viral origin, a putative causative pathogen is not identified in over a quarter of these febrile episodes. Using a de novo assembly sequencing approach, we report the detection (15.4%) of dicistroviruses (DicV) RNA in sera collected from 692 febrile Tanzanian children. In contrast, DicV RNA was only detected in 1/77 (1.3%) plasma samples from febrile Tanzanian adults, suggesting that children could represent the primary susceptible population. Estimated viral load by specific quantitative real-time RT-PCR assay ranged from < 1.32E3 to 1.44E7 viral RNA copies/mL serum. Three DicV full-length genomes were obtained, and a phylogenetic analyse on the capsid region showed the presence of two clusters representing tentative novel genus. Although DicV-positive cases were detected throughout the year, a significantly higher positivity rate was observed during the rainy season. This study reveals that novel DicV RNA is frequently detected in the blood of Tanzanian children, paving the way for further investigations to determine if DicV possibly represent a new agent in humans.
Druh dokumentu: Article
Other literature type
Popis souboru: application/pdf
Jazyk: English
ISSN: 2222-1751
DOI: 10.1080/22221751.2019.1603791
DOI: 10.5451/unibas-ep70293
DOI: 10.48350/160557
Přístupová URL adresa: https://www.tandfonline.com/doi/pdf/10.1080/22221751.2019.1603791?needAccess=true
https://pubmed.ncbi.nlm.nih.gov/30999808
https://doaj.org/article/a5be99b5fe8f4a26a02b3af3ed43f439
https://www.ncbi.nlm.nih.gov/pubmed/30999808
https://archive-ouverte.unige.ch/unige:122919/ATTACHMENT01
https://archive-ouverte.unige.ch/unige:122919
https://tandfonline.com/doi/pdf/10.1080/22221751.2019.1603791
https://europepmc.org/abstract/MED/30999808
https://serval.unil.ch/resource/serval:BIB_EC0E536871A0.P001/REF.pdf
https://archive-ouverte.unige.ch/unige:122919
https://serval.unil.ch/resource/serval:BIB_EC0E536871A0.P001/REF.pdf
https://serval.unil.ch/notice/serval:BIB_EC0E536871A0
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_EC0E536871A00
https://edoc.unibas.ch/70293/
https://boris.unibe.ch/160557/
Rights: CC BY
Přístupové číslo: edsair.doi.dedup.....f5a253c496fe3615282e921b144b85c9
Databáze: OpenAIRE
Popis
Abstrakt:Fever is the leading cause of paediatric outpatient consultations in Sub-Saharan Africa. Although most are suspected to be of viral origin, a putative causative pathogen is not identified in over a quarter of these febrile episodes. Using a de novo assembly sequencing approach, we report the detection (15.4%) of dicistroviruses (DicV) RNA in sera collected from 692 febrile Tanzanian children. In contrast, DicV RNA was only detected in 1/77 (1.3%) plasma samples from febrile Tanzanian adults, suggesting that children could represent the primary susceptible population. Estimated viral load by specific quantitative real-time RT-PCR assay ranged from < 1.32E3 to 1.44E7 viral RNA copies/mL serum. Three DicV full-length genomes were obtained, and a phylogenetic analyse on the capsid region showed the presence of two clusters representing tentative novel genus. Although DicV-positive cases were detected throughout the year, a significantly higher positivity rate was observed during the rainy season. This study reveals that novel DicV RNA is frequently detected in the blood of Tanzanian children, paving the way for further investigations to determine if DicV possibly represent a new agent in humans.
ISSN:22221751
DOI:10.1080/22221751.2019.1603791