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Prospects for cordycepin biosynthesis in microbial cell factories

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Názov: Prospects for cordycepin biosynthesis in microbial cell factories
Autori: Xiang Li, Rongshuai Jiang, Shenghou Wang, Chenyang Li, Yongping Xu, Shuying Li, Qian Li, Liang Wang
Zdroj: Frontiers in Chemical Engineering, Vol 6 (2024)
Informácie o vydavateľovi: Frontiers Media SA, 2024.
Rok vydania: 2024
Predmety: 2. Zero hunger, 0301 basic medicine, cordycepin, Technology, 0303 health sciences, 03 medical and health sciences, Chemical technology, non-conventional yeast, TP1-1185, biosynthesis, microbial cell factory, Cordyceps militaris
Popis: Cordycepin, an adenosine analog, exhibits diverse bioactivities and holds significant potential for applications in healthcare and agriculture. Fungi of the genus Cordyceps, such as Cordyceps militaris, can naturally produce cordycepin. Current sources of cordycepin primarily involve extraction from fruiting bodies or isolation from liquid fermentation using C. militaris, presenting challenges such as low production intensity, complex separation and purification systems, and high production costs, limiting industrial feasibility. Recent advancements have witnessed the utilization of various fungal chassis cells to successfully engineer heterologous biosynthetic platforms for cordycepin, such as Saccharomyces cerevisiae and unconventional yeasts, offering advantages of high yield, short fermentation cycles, and a broad substrate spectrum. This mini review summarizes the biosynthetic pathways of cordycepin and focused on the comparison of the characteristics, advantages, current performance and prospects for the microbial cell factories, analyzing potential targets for metabolic pathway modification and giving strategies in both genetic engineering and process engineering to enhance production intensity. The mini review particularly emphasizes the crucial role of chassis cell stress tolerance to the toxic product in determining cordycepin yield and highlights the urgent need for high-throughput screening methods for high-yield strains.
Druh dokumentu: Article
ISSN: 2673-2718
DOI: 10.3389/fceng.2024.1446454
Prístupová URL adresa: https://doaj.org/article/1a5db0fd80424557b7adb740640d9835
Rights: CC BY
Prístupové číslo: edsair.doi.dedup.....f43f39e5581cd20075e6e0c6c6175bb0
Databáza: OpenAIRE
Popis
Abstrakt:Cordycepin, an adenosine analog, exhibits diverse bioactivities and holds significant potential for applications in healthcare and agriculture. Fungi of the genus Cordyceps, such as Cordyceps militaris, can naturally produce cordycepin. Current sources of cordycepin primarily involve extraction from fruiting bodies or isolation from liquid fermentation using C. militaris, presenting challenges such as low production intensity, complex separation and purification systems, and high production costs, limiting industrial feasibility. Recent advancements have witnessed the utilization of various fungal chassis cells to successfully engineer heterologous biosynthetic platforms for cordycepin, such as Saccharomyces cerevisiae and unconventional yeasts, offering advantages of high yield, short fermentation cycles, and a broad substrate spectrum. This mini review summarizes the biosynthetic pathways of cordycepin and focused on the comparison of the characteristics, advantages, current performance and prospects for the microbial cell factories, analyzing potential targets for metabolic pathway modification and giving strategies in both genetic engineering and process engineering to enhance production intensity. The mini review particularly emphasizes the crucial role of chassis cell stress tolerance to the toxic product in determining cordycepin yield and highlights the urgent need for high-throughput screening methods for high-yield strains.
ISSN:26732718
DOI:10.3389/fceng.2024.1446454