Zobrazit v EDS

Mutagenesis techniques for evolutionary engineering of microbes – exploiting CRISPR-Cas, oligonucleotides, recombinases, and polymerases

Uloženo v:
Podrobná bibliografie
Název: Mutagenesis techniques for evolutionary engineering of microbes – exploiting CRISPR-Cas, oligonucleotides, recombinases, and polymerases
Autoři: Anna Zimmermann, Julian E. Prieto-Vivas, Karin Voordeckers, Changhao Bi, Kevin J. Verstrepen
Zdroj: Trends in Microbiology. 32:884-901
Informace o vydavateli: Elsevier BV, 2024.
Rok vydání: 2024
Témata: Biochemistry & Molecular Biology, 3101 Biochemistry and cell biology, Oligonucleotides, metabolic optimization, Microbiology, PRECISE, Recombinases, 1108 Medical Microbiology, engineering microbes, precision fermentation, directed evolution, 1S25923N#56755567, SCALE, REPAIR, Science & Technology, CONTINUOUS DIRECTED EVOLUTION, Bacteria, targeted mutagenesis, REARRANGEMENTS, TRANSFORMATION, 3. Good health, GENOME, 3107 Microbiology, Metabolic Engineering, Mutagenesis, Synthetic Biology, CRISPR-Cas Systems, Directed Molecular Evolution, Genetic Engineering, Life Sciences & Biomedicine, SYSTEM, 0605 Microbiology
Popis: The natural process of evolutionary adaptation is often exploited as a powerful tool to obtain microbes with desirable traits. For industrial microbes, evolutionary engineering is often used to generate variants that show increased yields or resistance to stressful industrial environments, thus obtaining superior microbial cell factories. However, even in large populations, the natural supply of beneficial mutations is typically low, which implies that obtaining improved microbes is often time-consuming and inefficient. To overcome this limitation, different techniques have been developed that boost mutation rates. While some of these methods simply increase the overall mutation rate across a genome, others use recent developments in DNA synthesis, synthetic biology, and CRISPR-Cas techniques to control the type and location of mutations. This review summarizes the most important recent developments and methods in the field of evolutionary engineering in model microorganisms. It discusses how both in vitro and in vivo approaches can increase the genetic diversity of the host, with a special emphasis on in vivo techniques for the optimization of metabolic pathways for precision fermentation.
Druh dokumentu: Article
Jazyk: English
ISSN: 0966-842X
DOI: 10.1016/j.tim.2024.02.006
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/38493013
Rights: CC BY NC
Přístupové číslo: edsair.doi.dedup.....f419593a8d054f7b8a59ae352469ec7d
Databáze: OpenAIRE
Popis
Abstrakt:The natural process of evolutionary adaptation is often exploited as a powerful tool to obtain microbes with desirable traits. For industrial microbes, evolutionary engineering is often used to generate variants that show increased yields or resistance to stressful industrial environments, thus obtaining superior microbial cell factories. However, even in large populations, the natural supply of beneficial mutations is typically low, which implies that obtaining improved microbes is often time-consuming and inefficient. To overcome this limitation, different techniques have been developed that boost mutation rates. While some of these methods simply increase the overall mutation rate across a genome, others use recent developments in DNA synthesis, synthetic biology, and CRISPR-Cas techniques to control the type and location of mutations. This review summarizes the most important recent developments and methods in the field of evolutionary engineering in model microorganisms. It discusses how both in vitro and in vivo approaches can increase the genetic diversity of the host, with a special emphasis on in vivo techniques for the optimization of metabolic pathways for precision fermentation.
ISSN:0966842X
DOI:10.1016/j.tim.2024.02.006