Gene Flow Between Chromosomal Forms of the Malaria Vector Anopheles funestus in Cameroon, Central Africa, and Its Relevance in Malaria Fighting
Uloženo v:
| Název: | Gene Flow Between Chromosomal Forms of the Malaria Vector Anopheles funestus in Cameroon, Central Africa, and Its Relevance in Malaria Fighting |
|---|---|
| Autoři: | Cohuet, Anna, Dia, I., Simard, Frédérique, Raymond, Michel, Rousset, François, Antonio-Nkondjio, C., Awono-Ambene, P.-H., Wondji, C.S., Fontenille, Didier |
| Přispěvatelé: | Laboratoire de Lutte Contre les Insectes Nuisibles (LIN), Institut Pasteur de Dakar, Pasteur Network (Réseau International des Instituts Pasteur), Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement IRD : UR226-Centre National de la Recherche Scientifique (CNRS), This study was funded by the French ministry of research throughout the PAL+ project., We acknowledge the villagers for their cooperation throughout the survey. |
| Zdroj: | Genetics. 169:301-311 |
| Informace o vydavateli: | Oxford University Press (OUP), 2005. |
| Rok vydání: | 2005 |
| Témata: | 0301 basic medicine, MESH: Geography, [SDV]Life Sciences [q-bio], MESH: Africa, MESH: Chromosomes/genetics, MESH: Genetics, Chromosomes, 03 medical and health sciences, MESH: Chromosome Inversion, Anopheles, MESH: Insect Vectors/classification, Animals, Humans, MESH: Animals, Africa, Central, MESH: Microsatellite Repeats/genetics, Cameroon, Central, MESH: Malaria/parasitology, 0303 health sciences, MESH: Humans, MESH: Genetic Variation, Geography, MESH: Cytogenetic Analysis, Genetic Variation, MESH: Cameroon, MESH: Insect Vectors/genetics, Population, Insect Vectors, Malaria, 3. Good health, MESH: Anopheles/genetics, Genetics, Population, Chromosome Inversion, Cytogenetic Analysis, Female, MESH: Female, Microsatellite Repeats |
| Popis: | Knowledge of population structure in a major vector species is fundamental to an understanding of malaria epidemiology and becomes crucial in the context of genetic control strategies that are being developed. Despite its epidemiological importance, the major African malaria vector Anopheles funestus has received far less attention than members of the Anopheles gambiae complex. Previous chromosomal data have shown a high degree of structuring within populations from West Africa and have led to the characterization of two chromosomal forms, “Kiribina” and “Folonzo.” In Central Africa, few data were available. We thus undertook assessment of genetic structure of An. funestus populations from Cameroon using chromosomal inversions and microsatellite markers. Microsatellite markers revealed no particular departure from panmixia within each local population and a genetic structure consistent with isolation by distance. However, cytogenetic studies demonstrated high levels of chromosomal heterogeneity, both within and between populations. Distribution of chromosomal inversions was not random and a cline of frequency was observed, according to ecotypic conditions. Strong deficiency of heterokaryotypes was found in certain localities in the transition area, indicating a subdivision of An. funestus in chromosomal forms. An. funestus microsatellite genetic markers located within the breakpoints of inversions are not differentiated in populations, whereas in An. gambiae inversions can affect gene flow at marker loci. These results are relevant to strategies for control of malaria by introduction of transgenes into populations of vectors. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 1943-2631 |
| DOI: | 10.1534/genetics.103.025031 |
| Přístupová URL adresa: | http://www.genetics.org/content/169/1/301.full.pdf https://pubmed.ncbi.nlm.nih.gov/15677749 http://www.evolutionhumaine.fr/michel/publis/pdf/cohuet_2005_genetics.pdf https://www.genetics.org/content/169/1/301 https://academic.oup.com/genetics/article/169/1/301/6060375 http://www.genetics.org/content/169/1/301 https://pubmed.ncbi.nlm.nih.gov/15677749/ https://europepmc.org/article/MED/15677749 |
| Rights: | OUP Standard Publication Reuse |
| Přístupové číslo: | edsair.doi.dedup.....f1af68aedd2fbcf9731878e1a6c27318 |
| Databáze: | OpenAIRE |
Full Text Finder 
Nájsť tento článok vo Web of Science | Abstrakt: | Knowledge of population structure in a major vector species is fundamental to an understanding of malaria epidemiology and becomes crucial in the context of genetic control strategies that are being developed. Despite its epidemiological importance, the major African malaria vector Anopheles funestus has received far less attention than members of the Anopheles gambiae complex. Previous chromosomal data have shown a high degree of structuring within populations from West Africa and have led to the characterization of two chromosomal forms, “Kiribina” and “Folonzo.” In Central Africa, few data were available. We thus undertook assessment of genetic structure of An. funestus populations from Cameroon using chromosomal inversions and microsatellite markers. Microsatellite markers revealed no particular departure from panmixia within each local population and a genetic structure consistent with isolation by distance. However, cytogenetic studies demonstrated high levels of chromosomal heterogeneity, both within and between populations. Distribution of chromosomal inversions was not random and a cline of frequency was observed, according to ecotypic conditions. Strong deficiency of heterokaryotypes was found in certain localities in the transition area, indicating a subdivision of An. funestus in chromosomal forms. An. funestus microsatellite genetic markers located within the breakpoints of inversions are not differentiated in populations, whereas in An. gambiae inversions can affect gene flow at marker loci. These results are relevant to strategies for control of malaria by introduction of transgenes into populations of vectors. |
|---|---|
| ISSN: | 19432631 |
| DOI: | 10.1534/genetics.103.025031 |