Citrate-Assisted Regulation of Protein Stability and Secretability from Synthetic Amyloids
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| Název: | Citrate-Assisted Regulation of Protein Stability and Secretability from Synthetic Amyloids |
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| Autoři: | Hèctor López-Laguna, Marianna T.P. Favaro, Sara Chellou-Bakkali, Eric Voltà-Durán, Eloi Parladé, Julieta Sánchez, José Luis Corchero, Ugutz Unzueta, Antonio Villaverde, Esther Vázquez |
| Zdroj: | ACS Appl Mater Interfaces UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| Informace o vydavateli: | American Chemical Society (ACS), 2025. |
| Rok vydání: | 2025 |
| Témata: | Recombinant proteins, Biomaterials, Endocrine-like function, Degradation, Toxicity, Àrees temàtiques de la UPC::Ciències de la salut, Nanoparticles, Cytotoxic proteins, Peptides and proteins, Protein secretion, Materials, Research Article |
| Popis: | The mammalian endocrine system uses functional amyloids as dynamic depots to store and release protein hormones into the bloodstream. Such depots, acting as secretory granules within the microscale, are formed in specialized cells by the coordination between the ionic, divalent form of zinc (Zn2+) and the imidazole ring from accessible His residues. The reversibility of such cross-linking events allows for the release of monomeric or oligomeric forms of the functional protein for biological activity. In vitro, and mimicking such a natural coordination process, synthetic amyloidal granules with secretory properties can be fabricated using selected therapeutic proteins as building blocks. Then, these microparticles act as delivery systems for endocrine-like, sustained protein release, with proven applicability in vaccinology, cancer therapy, regenerative medicine, and as antimicrobial agents. While the temporal profile in which the protein is leaked from the material might be highly relevant to clinically oriented applications, the fine control of such parameters remains unclear. We have explored here how the kinetics of protein release can be regulated by intervening in the storage formulation of the granules, through the concentration of citrate not only as a buffer component and protein stabilizer but also as a chelating agent. The citrate-assisted, time-prolonged regulatable release of proteins, in their functional form, opens a spectrum of possibilities to adjust the preparation of synthetic secretory granules to specific clinical needs. |
| Druh dokumentu: | Article Other literature type |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.4c20784 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/40009529 https://ddd.uab.cat/record/308730 |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/This article is licensed under CC-BY 4.0 |
| Přístupové číslo: | edsair.doi.dedup.....e99f2429aad4bffbbd72c1de6a9b2f7d |
| Databáze: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | The mammalian endocrine system uses functional amyloids as dynamic depots to store and release protein hormones into the bloodstream. Such depots, acting as secretory granules within the microscale, are formed in specialized cells by the coordination between the ionic, divalent form of zinc (Zn2+) and the imidazole ring from accessible His residues. The reversibility of such cross-linking events allows for the release of monomeric or oligomeric forms of the functional protein for biological activity. In vitro, and mimicking such a natural coordination process, synthetic amyloidal granules with secretory properties can be fabricated using selected therapeutic proteins as building blocks. Then, these microparticles act as delivery systems for endocrine-like, sustained protein release, with proven applicability in vaccinology, cancer therapy, regenerative medicine, and as antimicrobial agents. While the temporal profile in which the protein is leaked from the material might be highly relevant to clinically oriented applications, the fine control of such parameters remains unclear. We have explored here how the kinetics of protein release can be regulated by intervening in the storage formulation of the granules, through the concentration of citrate not only as a buffer component and protein stabilizer but also as a chelating agent. The citrate-assisted, time-prolonged regulatable release of proteins, in their functional form, opens a spectrum of possibilities to adjust the preparation of synthetic secretory granules to specific clinical needs. |
|---|---|
| ISSN: | 19448252 19448244 |
| DOI: | 10.1021/acsami.4c20784 |