Silk Sericin/Chitosan Supramolecular Multilayered Thin Films as Sustainable Cytocompatible Nanobiomaterials
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| Titel: | Silk Sericin/Chitosan Supramolecular Multilayered Thin Films as Sustainable Cytocompatible Nanobiomaterials |
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| Autoren: | Miguel Rosas, Cristiana F. V. Sousa, Ana Pereira, Adérito J. R. Amaral, Tamagno Pesqueira, Sónia G. Patrício, Sara Fateixa, Helena I. S. Nogueira, João F. Mano, Ana L. Oliveira, João Borges |
| Quelle: | Biomacromolecules. 26:296-310 |
| Verlagsinformationen: | American Chemical Society (ACS), 2024. |
| Publikationsjahr: | 2024 |
| Schlagwörter: | Chitosan, Sericins/chemistry, Layer-by-Layer technology, Cultured, Cells, Self-assembled nanobiomaterials, Bombyx/chemistry, Silk sericin, Cytocompatibility, Fibroblasts/drug effects, Silk/chemistry, Fourier Transform Infrared, Humans, Animals, Tissue Engineering/methods, Chitosan/chemistry, Nanofibrillar matrices, Spectroscopy, Biocompatible Materials/chemistry |
| Beschreibung: | Silk sericin (SS) has been widely discarded as a waste by the silk textile industry during the degumming process to obtain fibroin. However, in the past decade, an in-depth understanding of its properties and functions turned it into a high added-value biomaterial for biomedical applications. Herein, we report the molecular design and development of sustainable supramolecular multilayered nanobiomaterials encompassing SS and oppositely charged chitosan (CHT) through a combination of self-assembly and electrostatically driven layer-by-layer (LbL) assembly technology. The successful buildup of SS/CHT multilayered nanobiomaterials was demonstrated by the quartz crystal microbalance with dissipation monitoring and attenuated total reflectance-Fourier transform infrared spectroscopy, and the nanofilms' wettable properties and nanofibrillar-like topography were shown by water contact angle, atomic force microscopy, and scanning electron microscopy. In vitro assays demonstrated the cytocompatibility of the LbL nanofilms toward human primary dermal fibroblasts, holding great promise as biofunctional nanocoatings for drug/therapeutics/cell delivery, tissue engineering, and regenerative medicine. |
| Publikationsart: | Article |
| Sprache: | English |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.4c01146 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/39680042 https://ciencia.ucp.pt/en/publications/672ff990-6dec-4433-b38b-c7ea412ae1d0 https://doi.org/10.1021/acs.biomac.4c01146 http://hdl.handle.net/10773/43096 |
| Rights: | STM Policy #29 |
| Dokumentencode: | edsair.doi.dedup.....e93f6ffe0ec8d217a8af54b502ee170e |
| Datenbank: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstract: | Silk sericin (SS) has been widely discarded as a waste by the silk textile industry during the degumming process to obtain fibroin. However, in the past decade, an in-depth understanding of its properties and functions turned it into a high added-value biomaterial for biomedical applications. Herein, we report the molecular design and development of sustainable supramolecular multilayered nanobiomaterials encompassing SS and oppositely charged chitosan (CHT) through a combination of self-assembly and electrostatically driven layer-by-layer (LbL) assembly technology. The successful buildup of SS/CHT multilayered nanobiomaterials was demonstrated by the quartz crystal microbalance with dissipation monitoring and attenuated total reflectance-Fourier transform infrared spectroscopy, and the nanofilms' wettable properties and nanofibrillar-like topography were shown by water contact angle, atomic force microscopy, and scanning electron microscopy. In vitro assays demonstrated the cytocompatibility of the LbL nanofilms toward human primary dermal fibroblasts, holding great promise as biofunctional nanocoatings for drug/therapeutics/cell delivery, tissue engineering, and regenerative medicine. |
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| ISSN: | 15264602 15257797 |
| DOI: | 10.1021/acs.biomac.4c01146 |