Hippocampal P2X7 and A2A purinoceptors mediate cognitive impairment caused by long-lasting epileptic seizures
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| Titel: | Hippocampal P2X7 and A2A purinoceptors mediate cognitive impairment caused by long-lasting epileptic seizures |
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| Autoren: | Sun, Meng-Juan, Ren, Wen-Jing, Zhao, Ya-Fei, Li, Xuan, Khan, Muhammad Tahir, Cheng, Xin-Yi, Yin, Hai-Yan, Verkhratsky, Alexei, Engel, Tobias, Rubini, Patrizia, Tang, Yong, Illes, Peter |
| Quelle: | Theranostics Sun, M-J, Ren, W-J, Zhao, Y-F, Li, X, Khan, M T, Cheng, X-Y, Yin, H-Y, Verkhratsky, A, Engel, T, Rubini, P, Tang, Y & Illes, P 2025, 'Hippocampal P2X7 and A2A purinoceptors mediate cognitive impairment caused by long-lasting epileptic seizures', Theranostics, vol. 15, no. 7, pp. 3159-3184. https://doi.org/10.7150/thno.100365 |
| Verlagsinformationen: | Ivyspring International Publisher, 2025. |
| Publikationsjahr: | 2025 |
| Schlagwörter: | Male, Depression/metabolism, Hippocampus/metabolism, Cognitive Dysfunction/metabolism, Status Epilepticus/metabolism, Inbred C57BL, Hippocampus, Mice, Status Epilepticus, Seizures, Receptors, Animals, Cognitive Dysfunction, CA1 Region, Hippocampal/metabolism, Hippocampal/metabolism, CA1 Region, Hippocampal, Kainic Acid, Purinergic P2X7/metabolism, Animal, Depression, CA1 Region, Electroencephalography, Receptors, Purinergic P2X7/metabolism, Mice, Inbred C57BL, Disease Models, Animal, Kainic Acid/pharmacology, Disease Models, Seizures/metabolism, Receptors, Purinergic P2X7, Research Paper |
| Beschreibung: | Rationale: Cognitive impairment and depression are salient comorbidities of mesial temporal lobe epilepsy; it is still unclear whether this frequently drug resistant disease is a cause or consequence of hippocampal damage and its interplay with long-lasting seizure activity (status epilepticus; SE). Thus, a major therapeutic advance in this field is badly needed. Methods: We modeled enduring behavioral and electroencephalographic (EEG) seizures in mice by the intraperitoneal injection of kainic acid (KA), and measured the dynamics of the intracellular Ca2+ signals in the hippocampal CA1 area by fiber photometry. Learning and memory were controlled by the Morris Water-Maze and Novel Object Recognition tests on whole animals and by the induction of long-term potentiation in CA1 pyramidal neurons in brain slices. Depressive-like reactions were evaluated by the Tail Suspension, Forced Swim, and Sucrose Preference tests. Results: The intraperitoneal injection of the blood-brain permeable, highly selective, P2X7 and A2A receptor (R) antagonists, JNJ-47965567, and KW6002/SCH58261, respectively, counteracted the effects of KA-induced SE both on seizure activity and the increase of Ca2+ signals (as a measure of changes in the intracellular Ca2+ concentration) in neurons and astrocytes of the hippocampal CA1 area. In addition, these drugs also prevented the impairment of the hippocampus-dependent spatial and non-spatial learning abilities by KA-SE. The knockdown of P2X7Rs in CA1 astrocytes, but not neurons prevented the cognitive deterioration, suggesting that the release of astrocytic signaling molecules onto neighboring neurons might be the cause of this effect. In accordance with our observations, in hippocampal slices prepared from mice which underwent KA-SE, a selective sensitivity increases to the prototypic P2X7R agonist dibenzoyl-ATP (Bz-ATP) manifested in CA1 neurons. This sensitivity increase appeared to be due to a postsynaptic interference between P2X7Rs and the release of excitatory neurotransmitters during SE. In spite of a P2X7 and A2AR-mediated increase of Ca2+ signaling in the medial prefrontal cortex, no similar change was noted after KA-SE in depressive-like reactions or the open-field behavior. Conclusions: SE induced the release of ATP and adenosine from the hippocampus and in consequence decreased the cognitive abilities of mice. The pharmacological blockade of P2X7 and A2ARs prevented the SE-induced seizure activity and cognitive deterioration, but not depressive-like behavior. |
| Publikationsart: | Article Other literature type |
| Sprache: | English |
| ISSN: | 1838-7640 |
| DOI: | 10.7150/thno.100365 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/40083937 https://research.manchester.ac.uk/en/publications/322cd846-23cb-4ec0-8e4f-554884334b20 https://doi.org/10.7150/thno.100365 |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
| Dokumentencode: | edsair.doi.dedup.....df70de23e341ef29bbd4125be382052c |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Rationale: Cognitive impairment and depression are salient comorbidities of mesial temporal lobe epilepsy; it is still unclear whether this frequently drug resistant disease is a cause or consequence of hippocampal damage and its interplay with long-lasting seizure activity (status epilepticus; SE). Thus, a major therapeutic advance in this field is badly needed. Methods: We modeled enduring behavioral and electroencephalographic (EEG) seizures in mice by the intraperitoneal injection of kainic acid (KA), and measured the dynamics of the intracellular Ca2+ signals in the hippocampal CA1 area by fiber photometry. Learning and memory were controlled by the Morris Water-Maze and Novel Object Recognition tests on whole animals and by the induction of long-term potentiation in CA1 pyramidal neurons in brain slices. Depressive-like reactions were evaluated by the Tail Suspension, Forced Swim, and Sucrose Preference tests. Results: The intraperitoneal injection of the blood-brain permeable, highly selective, P2X7 and A2A receptor (R) antagonists, JNJ-47965567, and KW6002/SCH58261, respectively, counteracted the effects of KA-induced SE both on seizure activity and the increase of Ca2+ signals (as a measure of changes in the intracellular Ca2+ concentration) in neurons and astrocytes of the hippocampal CA1 area. In addition, these drugs also prevented the impairment of the hippocampus-dependent spatial and non-spatial learning abilities by KA-SE. The knockdown of P2X7Rs in CA1 astrocytes, but not neurons prevented the cognitive deterioration, suggesting that the release of astrocytic signaling molecules onto neighboring neurons might be the cause of this effect. In accordance with our observations, in hippocampal slices prepared from mice which underwent KA-SE, a selective sensitivity increases to the prototypic P2X7R agonist dibenzoyl-ATP (Bz-ATP) manifested in CA1 neurons. This sensitivity increase appeared to be due to a postsynaptic interference between P2X7Rs and the release of excitatory neurotransmitters during SE. In spite of a P2X7 and A2AR-mediated increase of Ca2+ signaling in the medial prefrontal cortex, no similar change was noted after KA-SE in depressive-like reactions or the open-field behavior. Conclusions: SE induced the release of ATP and adenosine from the hippocampus and in consequence decreased the cognitive abilities of mice. The pharmacological blockade of P2X7 and A2ARs prevented the SE-induced seizure activity and cognitive deterioration, but not depressive-like behavior. |
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| ISSN: | 18387640 |
| DOI: | 10.7150/thno.100365 |