Recent progress in adaptive laboratory evolution of industrial microorganisms
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| Název: | Recent progress in adaptive laboratory evolution of industrial microorganisms |
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| Autoři: | Guanglu Wang, Qian Li, Zhan Zhang, Xianzhong Yin, Bingyang Wang, Xuepeng Yang |
| Zdroj: | J Ind Microbiol Biotechnol |
| Informace o vydavateli: | Oxford University Press (OUP), 2022. |
| Rok vydání: | 2022 |
| Témata: | 0301 basic medicine, 0303 health sciences, 03 medical and health sciences, Metabolic Engineering, Mutation, Biotechnology Methods, Metabolic Networks and Pathways |
| Popis: | Adaptive laboratory evolution (ALE) is a technique for the selection of strains with better phenotypes by long-term culture under a specific selection pressure or growth environment. Because ALE does not require detailed knowledge of a variety of complex and interactive metabolic networks, and only needs to simulate natural environmental conditions in the laboratory to design a selection pressure, it has the advantages of broad adaptability, strong practicability, and more convenient transformation of strains. In addition, ALE provides a powerful method for studying the evolutionary forces that change the phenotype, performance, and stability of strains, resulting in more productive industrial strains with beneficial mutations. In recent years, ALE has been widely used in the activation of specific microbial metabolic pathways and phenotypic optimization, the efficient utilization of specific substrates, the optimization of tolerance to toxic substance, and the biosynthesis of target products, which is more conducive to the production of industrial strains with excellent phenotypic characteristics. In this paper, typical examples of ALE applications in the development of industrial strains and the research progress of this technology are reviewed, followed by a discussion of its development prospects. |
| Druh dokumentu: | Article Other literature type |
| Jazyk: | English |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1093/jimb/kuac023 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/36323428 |
| Rights: | CC BY NC ND |
| Přístupové číslo: | edsair.doi.dedup.....de90a1d68998084069874b6dcc9d538b |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Adaptive laboratory evolution (ALE) is a technique for the selection of strains with better phenotypes by long-term culture under a specific selection pressure or growth environment. Because ALE does not require detailed knowledge of a variety of complex and interactive metabolic networks, and only needs to simulate natural environmental conditions in the laboratory to design a selection pressure, it has the advantages of broad adaptability, strong practicability, and more convenient transformation of strains. In addition, ALE provides a powerful method for studying the evolutionary forces that change the phenotype, performance, and stability of strains, resulting in more productive industrial strains with beneficial mutations. In recent years, ALE has been widely used in the activation of specific microbial metabolic pathways and phenotypic optimization, the efficient utilization of specific substrates, the optimization of tolerance to toxic substance, and the biosynthesis of target products, which is more conducive to the production of industrial strains with excellent phenotypic characteristics. In this paper, typical examples of ALE applications in the development of industrial strains and the research progress of this technology are reviewed, followed by a discussion of its development prospects. |
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| ISSN: | 14765535 13675435 |
| DOI: | 10.1093/jimb/kuac023 |