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Dehydroascorbic acid prevents oxidative cell death through a glutathione pathway in primary astrocytes

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Název: Dehydroascorbic acid prevents oxidative cell death through a glutathione pathway in primary astrocytes
Autoři: Eun Joo Baik, Bae Hwan Lee, Eun Joo Kim, Ran Won, Taik Sang Nahm, Yong Gou Park, Se Jung Jung
Přispěvatelé: Eun Joo Kim, Yong Gou Park, Eun Joo Baik, Se Jung Jung, Ran Won, Taik Sang Nahm, Bae Hwan Lee, Kim, Eun Joo, Nam, Taick Sang, Park, Yong Gou, Lee, Bae Hwan, Jung, Se Jung
Zdroj: Journal of Neuroscience Research. 79:670-679
Informace o vydavateli: Wiley, 2005.
Rok vydání: 2005
Témata: 0301 basic medicine, Time Factors, Astrocytes/physiology, Membrane Potentials/drug effects, Signal Transduction/drug effects, Membrane Potentials, Glutathione Reductase/metabolism, Mice, Cell Death/drug effects, Drug Interactions, Glutathione/metabolism, Cells, Cultured, Microscopy, Mice, Inbred ICR, 0303 health sciences, Cultured, Microscopy, Confocal, Cell Death, dehydroascorbic acid, Brain, Inbred ICR, Dehydroascorbic Acid, Glutathione, Mitochondria/drug effects, Mitochondria, Confocal/methods, Glutathione Reductase, Oxidative Stress/drug effects, ascorbic acid, Drug, Hydrogen Peroxide/pharmacology, Cells, Oxidoreductases/metabolism, pentose phosphate pathway, gluta-thione, Oxidative Stress/physiology, hydrogen peroxide, Astrocytes/drug effects, Dose-Response Relationship, 03 medical and health sciences, Reactive Oxygen Species/metabolism, Mitochondria/physiology, Animals, Analysis of Variance, Glutathione Peroxidase, Dose-Response Relationship, Drug, Signal Transduction/physiology, Dehydroascorbic Acid/pharmacology, Glutathione Peroxidase/metabolism, Hydrogen Peroxide, Newborn, Oxidative Stress, Brain/cytology, Animals, Newborn, Astrocytes
Popis: Ascorbic acid (AA) is a well‐known antioxidant. It also has pro‐oxidant effects, however, in the presence of free transition metals. Because of the pro‐oxidant effects of AA, dehydroascorbic acid (DHA), an oxidized form of AA, has been used as a substitute for AA. DHA has been shown recently to have a protective effect in an experimental stroke model. This study was carried out to determine if DHA has different effects from AA on hydrogen peroxide (H2O2)‐induced oxidative cell death in primary astrocytes. DHA was found to prevent cell death and reverse mitochondrial dysfunction after exposure to H2O2. DHA significantly increased the glutathione peroxidase (GPx) and glutathione reductase (GR) activities 1 hr after H2O2 exposure. Moreover, DHA not only reversed the decrease in the glutathione (GSH) levels, but also significantly enhanced it by stimulating the pentose phosphate pathway (PPP) 15 hr after H2O2 exposure. DHA also reduced production of reactive oxygen species (ROS) after H2O2 exposure. In contrast, AA accelerated H2O2‐induced cell death. To determine if the pro‐oxidant effect of AA is related to iron, the effect of AA on cell death was examined using an iron chelator, desferrioxamine. Even though co‐pretreatment with AA and desferrioxamine could abrogate the aggravating effects of AA on H2O2‐induced cell death at early stages, it could not prevent H2O2‐induced cell death over a 24‐hr period. These results suggest that DHA has distinct effects from AA and prevent H2O2‐induced cell death by increasing the GSH levels mediated by the GPx and GR activities and PPP. © 2005 Wiley‐Liss, Inc.
Druh dokumentu: Article
Jazyk: English
ISSN: 1097-4547
0360-4012
DOI: 10.1002/jnr.20384
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/15668957
https://www.ncbi.nlm.nih.gov/pubmed/15668957
https://onlinelibrary.wiley.com/doi/10.1002/jnr.20384
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147426
Rights: Wiley Online Library User Agreement
CC BY NC ND
Přístupové číslo: edsair.doi.dedup.....ddd563e0bf699d0b4f0df6914f86533a
Databáze: OpenAIRE
Popis
Abstrakt:Ascorbic acid (AA) is a well‐known antioxidant. It also has pro‐oxidant effects, however, in the presence of free transition metals. Because of the pro‐oxidant effects of AA, dehydroascorbic acid (DHA), an oxidized form of AA, has been used as a substitute for AA. DHA has been shown recently to have a protective effect in an experimental stroke model. This study was carried out to determine if DHA has different effects from AA on hydrogen peroxide (H2O2)‐induced oxidative cell death in primary astrocytes. DHA was found to prevent cell death and reverse mitochondrial dysfunction after exposure to H2O2. DHA significantly increased the glutathione peroxidase (GPx) and glutathione reductase (GR) activities 1 hr after H2O2 exposure. Moreover, DHA not only reversed the decrease in the glutathione (GSH) levels, but also significantly enhanced it by stimulating the pentose phosphate pathway (PPP) 15 hr after H2O2 exposure. DHA also reduced production of reactive oxygen species (ROS) after H2O2 exposure. In contrast, AA accelerated H2O2‐induced cell death. To determine if the pro‐oxidant effect of AA is related to iron, the effect of AA on cell death was examined using an iron chelator, desferrioxamine. Even though co‐pretreatment with AA and desferrioxamine could abrogate the aggravating effects of AA on H2O2‐induced cell death at early stages, it could not prevent H2O2‐induced cell death over a 24‐hr period. These results suggest that DHA has distinct effects from AA and prevent H2O2‐induced cell death by increasing the GSH levels mediated by the GPx and GR activities and PPP. © 2005 Wiley‐Liss, Inc.
ISSN:10974547
03604012
DOI:10.1002/jnr.20384