Pseudomonas aeruginosa Soluble Pyocins as Antibacterial Weapons
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| Title: | Pseudomonas aeruginosa Soluble Pyocins as Antibacterial Weapons |
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| Authors: | Cornelis, Pierre, Dingemans, Jozef, Baysse, Christine |
| Contributors: | Chard-Hutchinson, Xavier, Vriendenkring VUB, Microbiology, Faculty of Sciences and Bioengineering Sciences |
| Source: | Methods in Molecular Biology ISBN: 9781071634721 |
| Publisher Information: | Springer US, 2023. |
| Publication Year: | 2023 |
| Subject Terms: | Pyocins, Anti-Bacterial Agents/chemistry, S6, Bacteriocins/metabolism, S3, S5, S4, Anti-Bacterial Agents, 3. Good health, Pseudomonas aeruginosa/metabolism, Immunity protein, [SDV] Life Sciences [q-bio], Bacteriocins, Pseudomonas Infections/drug therapy, Pyocins S2, Escherichia coli/metabolism, Pseudomonas aeruginosa, Escherichia coli, Humans, Pseudomonas Infections, Pyocins/pharmacology, Soluble pyocins, Receptor binding domain |
| Description: | Pseudomonas aeruginosa is an opportunistic pathogen causing nosocomial infections and associated with lung infections in cystic fibrosis (CF) patients (Lyczak et al., Microbes Infect 2:1051-1060, 2000). Multiple drug-resistant P. aeruginosa strains pose a serious problem because of antibiotic treatment failure. There is therefore a need for alternative anti-Pseudomonas molecules. Soluble pyocins (S-pyocins) are bacteriocins produced by P. aeruginosa strains that kill sensitive strains of the same species. These bacteriocins and their immunity gene are easily cloned and expressed in E. coli and their activity spectrum against different P. aeruginosa strains can be tested. In this chapter, we describe the procedures for cloning, expression, and sensitivity testing of two different S-pyocins. We also describe how to identify their receptor binding domain in sensitive strains, how to construct chimeric pyocins with extended activity spectra, and how to identify new pyocins in genomes by multiplex PCR. |
| Document Type: | Part of book or chapter of book Article |
| Language: | English |
| DOI: | 10.1007/978-1-0716-3473-8_9 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/37819519 https://hal.science/hal-04313709v1 https://doi.org/10.1007/978-1-0716-3473-8_9 https://biblio.vub.ac.be/vubir/pseudomonas-aeruginosa-soluble-pyocins-as-antibacterial-weapons(c2171178-55a4-4fc7-9ee1-733eafaefe9b).html |
| Rights: | Springer Nature TDM |
| Accession Number: | edsair.doi.dedup.....da4e9ff9d4adfb82063731325c65f3f5 |
| Database: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstract: | Pseudomonas aeruginosa is an opportunistic pathogen causing nosocomial infections and associated with lung infections in cystic fibrosis (CF) patients (Lyczak et al., Microbes Infect 2:1051-1060, 2000). Multiple drug-resistant P. aeruginosa strains pose a serious problem because of antibiotic treatment failure. There is therefore a need for alternative anti-Pseudomonas molecules. Soluble pyocins (S-pyocins) are bacteriocins produced by P. aeruginosa strains that kill sensitive strains of the same species. These bacteriocins and their immunity gene are easily cloned and expressed in E. coli and their activity spectrum against different P. aeruginosa strains can be tested. In this chapter, we describe the procedures for cloning, expression, and sensitivity testing of two different S-pyocins. We also describe how to identify their receptor binding domain in sensitive strains, how to construct chimeric pyocins with extended activity spectra, and how to identify new pyocins in genomes by multiplex PCR. |
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| DOI: | 10.1007/978-1-0716-3473-8_9 |