Serum Reactivity Against Herpes Simplex Virus Type 1 UL48 Protein in Behçet’s Disease Patients and a Behçet’s Disease-like Mouse Model
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| Název: | Serum Reactivity Against Herpes Simplex Virus Type 1 UL48 Protein in Behçet’s Disease Patients and a Behçet’s Disease-like Mouse Model |
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| Autoři: | Zhenlong Zheng, Dongsik Bang, Keun Jae Ahn, Sung Bin Cho, Seonghyang Sohn |
| Přispěvatelé: | Zhenlong Zheng, Seonghyang Sohn, Keun Jae Ahn, Dongsik Bang, Sung Bin Cho, Bang, Dong Sik, Cho, Sung Bin |
| Zdroj: | Acta Dermato Venereologica. 95:952-958 |
| Informace o vydavateli: | MJS Publishing, Medical Journals Sweden AB, 2015. |
| Rok vydání: | 2015 |
| Témata: | Adult, Male, 0301 basic medicine, Microvessels/immunology, Herpesvirus 1, Human, Cross Reactions, Behçet's disease-like mouse model, Young Adult, Behçet's disease, Mice, Viral Proteins, 03 medical and health sciences, proteomics, Animals, Humans, UL48, Recombinant Proteins/immunology, Human/immunology, Mice, Inbred ICR, 0303 health sciences, Behcet Syndrome/blood, Immunoglobulin G/blood, Animal, Herpesvirus 1, Behcet Syndrome, HSC70 Heat-Shock Proteins, Endothelial Cells/immunology, Endothelial Cells, Middle Aged, herpes simplex virus, Inbred ICR, heat shock cognate 71 kDa protein, Recombinant Proteins, Immunoglobulin A, 3. Good health, Immunoglobulin A/blood, Disease Models, Animal, Case-Control Studies, Immunoglobulin G, Disease Models, Microvessels, Female, HSC70 Heat-Shock Proteins/immunology, Viral Proteins/immunology |
| Popis: | Herpes simplex virus (HSV) infection is a possible pathogenic factor in Behçet's disease (BD). Using proteomics analysis, this study detected a target HSV protein. Serum IgA and IgG reactivities against the identified protein were evaluated in patients with BD and in BD-like mice. A total of 4 protein bands generated by immunoprecipitation were analysed by proteomics, and HSV UL48 was commonly found in both IgA- and IgG-reactive protein bands. Compared with controls, patients with BD and BD-like mice exhibited higher titres of IgA reacting with recombinant HSV UL48 protein. Further proteomics analysis revealed that human heat shock cognate 71 kDa protein (Hsc71) is a cross-reacting target antigen against anti-HSV UL48 antibody. In addition, our data demonstrated a very strong association between serum IgG reactivity against recombinant human Hsc71 and recombinant HSV UL48 in patients with BD. We suggest that HSV infection and impaired human Hsc71 activity may be associated with the activation of autoreactive lymphocytes. |
| Druh dokumentu: | Article |
| Popis souboru: | 952~958 |
| Jazyk: | English |
| ISSN: | 0001-5555 |
| DOI: | 10.2340/00015555-2127 |
| Přístupová URL adresa: | https://www.medicaljournals.se/acta/download/10.2340/00015555-2127/ https://pubmed.ncbi.nlm.nih.gov/25916670 https://pubmed.ncbi.nlm.nih.gov/25916670/ https://www.ncbi.nlm.nih.gov/pubmed/25916670 https://www.medicaljournals.se/acta/content/?doi=10.2340/00015555-2127 |
| Rights: | CC BY NC CC BY NC ND |
| Přístupové číslo: | edsair.doi.dedup.....d5469ac091b1cbc8fdeacdcdb2c0e3d5 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Herpes simplex virus (HSV) infection is a possible pathogenic factor in Behçet's disease (BD). Using proteomics analysis, this study detected a target HSV protein. Serum IgA and IgG reactivities against the identified protein were evaluated in patients with BD and in BD-like mice. A total of 4 protein bands generated by immunoprecipitation were analysed by proteomics, and HSV UL48 was commonly found in both IgA- and IgG-reactive protein bands. Compared with controls, patients with BD and BD-like mice exhibited higher titres of IgA reacting with recombinant HSV UL48 protein. Further proteomics analysis revealed that human heat shock cognate 71 kDa protein (Hsc71) is a cross-reacting target antigen against anti-HSV UL48 antibody. In addition, our data demonstrated a very strong association between serum IgG reactivity against recombinant human Hsc71 and recombinant HSV UL48 in patients with BD. We suggest that HSV infection and impaired human Hsc71 activity may be associated with the activation of autoreactive lymphocytes. |
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| ISSN: | 00015555 |
| DOI: | 10.2340/00015555-2127 |