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Melatonin protects against oxidative stress in granular corneal dystrophy type 2 corneal fibroblasts by mechanisms that involve membrane melatonin receptors: Melatonin protects GCD2 corneal fibroblasts

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Title: Melatonin protects against oxidative stress in granular corneal dystrophy type 2 corneal fibroblasts by mechanisms that involve membrane melatonin receptors: Melatonin protects GCD2 corneal fibroblasts
Authors: Tae Im Kim, Eung Kweon Kim, Hyunmi Ryu, Seung Il Choi, Shorafidinkhuja Dadakhujaev
Contributors: Seung-Il Choi, Shorafidinkhuja Dadakhujaev, Hyunmi Ryu, Tae im Kim, Eung Kweon Kim, Kim, Eung Kweon, Kim, Tae Im, Dadakhujaev, Shorafidinkhuja, Ryu, Hyun Mi, Choi, Seung Il
Source: Journal of Pineal Research. 51:94-103
Publisher Information: Wiley, 2011.
Publication Year: 2011
Subject Terms: 0301 basic medicine, Hereditary/drug therapy, corneal fibroblasts, Receptors, Melatonin, melatonin, Antioxidants, Superoxide Dismutase/metabolism, Glutathione Reductase/metabolism, Cornea, Receptors, oxidative stress, Cells, Cultured, Melatonin, Fibroblasts/metabolism, Corneal Dystrophies, Hereditary, 0303 health sciences, Cultured, Blotting, Melatonin/pharmacology, Paraquat/pharmacology, Catalase, Flow Cytometry, Immunohistochemistry, 3. Good health, Cornea/pathology, Glutathione Reductase, Oxidative Stress/drug effects, Cornea/drug effects, Melatonin/metabolism, Western, Cell Survival/drug effects, Paraquat, Hereditary/metabolism, Corneal Dystrophies, Cell Survival, Cells, Blotting, Western, 03 medical and health sciences, Reactive Oxygen Species/metabolism, melatonin receptors, Humans, Hereditary/pathology, Hydrogen Peroxide/metabolism, granular corneal dystrophy type 2, Analysis of Variance, Superoxide Dismutase, Catalase/metabolism, Hydrogen Peroxide, Fibroblasts, Fibroblasts/pathology, Fibroblasts/drug effects, Oxidative Stress, Antioxidants/pharmacology, Reactive Oxygen Species
Description: Considering that oxidative stress plays a role in corneal fibroblast degeneration during granular corneal dystrophy type 2 (GCD2) and melatonin is an effective antioxidant, we examined the ability of melatonin to protect against oxidative stress-induced cell death of primary cultured normal and GCD2-homozygous corneal fibroblasts. Melatonin treatment protected primary cultured normal and GCD2 corneal fibroblasts from paraquat (PQ)-induced oxidative stress and caused increased expression levels of Cu/Zn-superoxide dismutase (SOD1) and glutathione reductase (GR) in both types of cells. Interestingly, catalase expression increased in normal corneal fibroblasts, but decreased in GCD2 corneal fibroblasts after melatonin treatment. Melatonin also reduced the levels of intracellular reactive oxygen species and H(2)O(2) in both cell types. In addition, the selective melatonin receptor antagonist luzindole blocked melatonin-induced expression of SOD1 and GR. The expression levels of melatonin receptors 1A (MT1) and 1B (MT2) were significantly higher in GCD2 corneal fibroblasts than in normal cells. These results suggest that increased expression of melatonin receptors may be involved in the defense mechanisms against oxidative stress in GCD2 corneal fibroblasts, and melatonin may have potential therapeutic implications for GCD2 treatment.
Document Type: Article
File Description: 94~103
Language: English
ISSN: 0742-3098
DOI: 10.1111/j.1600-079x.2011.00866.x
Access URL: https://pubmed.ncbi.nlm.nih.gov/21392093
https://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.2011.00866.x/full
https://yonsei.pure.elsevier.com/en/publications/melatonin-protects-against-oxidative-stress-in-granular-corneal-d
https://pubmed.ncbi.nlm.nih.gov/21392093/
https://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.2011.00866.x
https://europepmc.org/article/MED/21392093
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.2011.00866.x/full
Rights: Wiley TDM
CC BY NC ND
Accession Number: edsair.doi.dedup.....d4944b741810b3c6fe42de9149ae3f3c
Database: OpenAIRE
Description
Abstract:Considering that oxidative stress plays a role in corneal fibroblast degeneration during granular corneal dystrophy type 2 (GCD2) and melatonin is an effective antioxidant, we examined the ability of melatonin to protect against oxidative stress-induced cell death of primary cultured normal and GCD2-homozygous corneal fibroblasts. Melatonin treatment protected primary cultured normal and GCD2 corneal fibroblasts from paraquat (PQ)-induced oxidative stress and caused increased expression levels of Cu/Zn-superoxide dismutase (SOD1) and glutathione reductase (GR) in both types of cells. Interestingly, catalase expression increased in normal corneal fibroblasts, but decreased in GCD2 corneal fibroblasts after melatonin treatment. Melatonin also reduced the levels of intracellular reactive oxygen species and H(2)O(2) in both cell types. In addition, the selective melatonin receptor antagonist luzindole blocked melatonin-induced expression of SOD1 and GR. The expression levels of melatonin receptors 1A (MT1) and 1B (MT2) were significantly higher in GCD2 corneal fibroblasts than in normal cells. These results suggest that increased expression of melatonin receptors may be involved in the defense mechanisms against oxidative stress in GCD2 corneal fibroblasts, and melatonin may have potential therapeutic implications for GCD2 treatment.
ISSN:07423098
DOI:10.1111/j.1600-079x.2011.00866.x