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Protists are key players in the utilization of protein nitrogen in the arbuscular mycorrhizal hyphosphere

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Titel: Protists are key players in the utilization of protein nitrogen in the arbuscular mycorrhizal hyphosphere
Autoren: Anukool Vaishnav, Martin Rozmoš, Michala Kotianová, Hana Hršelová, Petra Bukovská, Jan Jansa
Quelle: New Phytol
Verlagsinformationen: Wiley, 2025.
Publikationsjahr: 2025
Schlagwörter: Nitrogen Isotopes, Nitrogen, temporal dynamics, Research, hyphosphere, organic nitrogen, Hyphae, stable isotopes, Eukaryota, multitrophic interactions, Chitin, acanthamoeba-castellanii, arbuscular mycorrhizal fungus, Plant Roots, soil, bacterial community composition, protozoa, plant-growth, Mycorrhizae, fungi, quantitative real-time PCR, microbial loop, rhizosphere, competition, Plant Proteins
Beschreibung: Summary While largely depending on other microorganisms for nitrogen (N) mineralization, arbuscular mycorrhizal fungi (AMF) can transfer N from organic sources to their host plants. Here, we compared N acquisition by the AMF hyphae from chitin and protein sources and assessed the effects of microbial interactions in the hyphosphere. We employed in vitro compartmented microcosms, each containing three distinct hyphosphere compartments amended with different N sources (protein, chitin, or ammonium chloride), one of which was enriched with 15N isotope. All hyphosphere compartments were supplied with Paenibacillus bacteria, with or without the protist Polysphondylium pallidum. We measured the effect of these model microbiomes on the efficiency of 15N transfer to roots via the AMF hyphae. We found that the hyphae efficiently took up N from ammonium chloride, competing strongly with bacteria and protists. Mobilization of 15N from chitin and protein was facilitated by bacteria and protists, respectively. Notably, AMF priming significantly affected the abundance of bacteria and protists in hyphosphere compartments and promoted mineralization of protein N by protists. Subsequently, this N was transferred into roots. Our results provide the first unequivocal evidence that roots can acquire N from proteins present in the AMF hyphosphere and that protists may play a crucial role in protein N mineralization.
Publikationsart: Article
Other literature type
Sprache: English
ISSN: 1469-8137
0028-646X
DOI: 10.1111/nph.70153
Zugangs-URL: https://pubmed.ncbi.nlm.nih.gov/40259857
https://hdl.handle.net/11104/0366675
Rights: CC BY
Dokumentencode: edsair.doi.dedup.....d296966d35f16f8e26e5a6df108d0eba
Datenbank: OpenAIRE
Beschreibung
Abstract:Summary While largely depending on other microorganisms for nitrogen (N) mineralization, arbuscular mycorrhizal fungi (AMF) can transfer N from organic sources to their host plants. Here, we compared N acquisition by the AMF hyphae from chitin and protein sources and assessed the effects of microbial interactions in the hyphosphere. We employed in vitro compartmented microcosms, each containing three distinct hyphosphere compartments amended with different N sources (protein, chitin, or ammonium chloride), one of which was enriched with 15N isotope. All hyphosphere compartments were supplied with Paenibacillus bacteria, with or without the protist Polysphondylium pallidum. We measured the effect of these model microbiomes on the efficiency of 15N transfer to roots via the AMF hyphae. We found that the hyphae efficiently took up N from ammonium chloride, competing strongly with bacteria and protists. Mobilization of 15N from chitin and protein was facilitated by bacteria and protists, respectively. Notably, AMF priming significantly affected the abundance of bacteria and protists in hyphosphere compartments and promoted mineralization of protein N by protists. Subsequently, this N was transferred into roots. Our results provide the first unequivocal evidence that roots can acquire N from proteins present in the AMF hyphosphere and that protists may play a crucial role in protein N mineralization.
ISSN:14698137
0028646X
DOI:10.1111/nph.70153