Metabolite profiling of natural substances in human: in vitro study from fecal bacteria to colon carcinoma cells (Caco-2): in vitro study from fecal bacteria to colon carcinoma cells (Caco-2)
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| Názov: | Metabolite profiling of natural substances in human: in vitro study from fecal bacteria to colon carcinoma cells (Caco-2): in vitro study from fecal bacteria to colon carcinoma cells (Caco-2) |
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| Autori: | Franz Gabor, Milan Kolar, Barbora Papoušková, Eva Anzenbacherova, Julia Clara Gausterer, Pavel Anzenbacher, Stefan Poschner, Zuzana Racova, Pavla Kucova |
| Zdroj: | The Journal of Nutritional Biochemistry. 85:108482 |
| Informácie o vydavateľovi: | Elsevier BV, 2020. |
| Rok vydania: | 2020 |
| Predmety: | 0301 basic medicine, Feces/microbiology, Polyphenols/metabolism, BIOAVAILABILITY, BIOLOGICAL-ACTIVITIES, ENTEROCOCCUS-FAECALIS, ANTHOCYANINS, LACTIC-ACID BACTERIA, Fecal bacteria, Feces, 03 medical and health sciences, 303002 Bakteriologie, ABSORPTION, Metabolomics, BETA-KETOADIPATE PATHWAY, Humans, Glycosides/metabolism, human, Glycosides, Intestinal Mucosa, DIETARY FLAVONOIDS, Flavonoids, O-METHYLTRANSFERASE, 0303 health sciences, Bacteria, Flavonoids/metabolism, 16S RIBOSOMAL-RNA, Polyphenols, Caco-2, Colonic Neoplasms/metabolism, 3. Good health, Metabolism, Bacteria/metabolism, Colonic Neoplasms, Metabolome, 303002 Bacteriology, Caco-2 Cells, Intestinal Mucosa/metabolism, Metabolic Networks and Pathways, Human |
| Popis: | Flavonoids, including anthocyanins, are polyphenolic compounds present in fruits, vegetables and dietary supplements. They can be absorbed from the intestine to the bloodstream or pass into the large intestine. Various bacterial species and enzymes are present along the entire intestine. The aim of the present work was to investigate the intestinal metabolism of selected dietary polyphenol and polyphenol glycosides (quercetin, cyanidin-3-O-glucoside, cyanidin-3-O-galactoside, and delphinidin-3-O-galactoside) by human fecal bacteria. Moreover, the metabolism of metabolites formed from these compounds in human colon carcinoma cells (Caco-2) was also point of the interest. Test compounds were added to fresh human stool in broth or to Caco-2 cells in medium and then incubated for 6 or 20 h at 37°C. After incubation, samples were prepared for LC/MS determination. Main metabolic pathways were deglycosylation, hydrogenation, methylation, hydroxylation, and decomposition. 2,4,5-trihydroxybenzaldehyde, as a metabolite of cyanidin glycosides, was detected after incubation for the first time. Metabolites formed by fecal bacteria were further glucuronidated or methylated by intestinal enzymes. This metabolite profiling of natural compounds has helped to better understand the complex metabolism in the human intestine and this work also has shown the connection of metabolism of natural substances by intestinal bacteria followed by metabolism in intestinal cells. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2020.108482 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/32801030 https://ucrisportal.univie.ac.at/de/publications/ac76c996-1a9d-485a-be1b-a505b11f80d6 https://doi.org/10.1016/j.jnutbio.2020.108482 https://www.sciencedirect.com/science/article/pii/S0955286320305143 https://pubmed.ncbi.nlm.nih.gov/32801030/ https://www.ncbi.nlm.nih.gov/pubmed/32801030 |
| Rights: | Elsevier TDM |
| Prístupové číslo: | edsair.doi.dedup.....d26d76e74a10589d8516b98aab14320b |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Flavonoids, including anthocyanins, are polyphenolic compounds present in fruits, vegetables and dietary supplements. They can be absorbed from the intestine to the bloodstream or pass into the large intestine. Various bacterial species and enzymes are present along the entire intestine. The aim of the present work was to investigate the intestinal metabolism of selected dietary polyphenol and polyphenol glycosides (quercetin, cyanidin-3-O-glucoside, cyanidin-3-O-galactoside, and delphinidin-3-O-galactoside) by human fecal bacteria. Moreover, the metabolism of metabolites formed from these compounds in human colon carcinoma cells (Caco-2) was also point of the interest. Test compounds were added to fresh human stool in broth or to Caco-2 cells in medium and then incubated for 6 or 20 h at 37°C. After incubation, samples were prepared for LC/MS determination. Main metabolic pathways were deglycosylation, hydrogenation, methylation, hydroxylation, and decomposition. 2,4,5-trihydroxybenzaldehyde, as a metabolite of cyanidin glycosides, was detected after incubation for the first time. Metabolites formed by fecal bacteria were further glucuronidated or methylated by intestinal enzymes. This metabolite profiling of natural compounds has helped to better understand the complex metabolism in the human intestine and this work also has shown the connection of metabolism of natural substances by intestinal bacteria followed by metabolism in intestinal cells. |
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| ISSN: | 09552863 |
| DOI: | 10.1016/j.jnutbio.2020.108482 |