Sustained protein release from hydrogel microparticles using layer-by-layer (LbL) technology
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| Název: | Sustained protein release from hydrogel microparticles using layer-by-layer (LbL) technology |
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| Autoři: | Sakr, Omar, Jordan, Olivier, Borchard, Gerrit |
| Zdroj: | Drug Delivery. 23:2747-2755 |
| Informace o vydavateli: | Informa UK Limited, 2015. |
| Rok vydání: | 2015 |
| Témata: | 0301 basic medicine, Protein activity, Polymers, Chemistry, Pharmaceutical, Lysozyme, Delayed-Action Preparations / chemistry, Muramidase / chemistry, Hydrogel, Polyethylene Glycol Dimethacrylate, 12. Responsible consumption, Excipients, 03 medical and health sciences, Drug Delivery Systems, Proteins / chemistry, Coated Materials, Biocompatible, Drug Delivery Systems / methods, Technology, Pharmaceutical, Protein delivery, Excipients / chemistry, Proteins, Chemistry, Pharmaceutical / methods, Layer-by-layer, Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry, 3. Good health, Technology, Pharmaceutical / methods, Polymers / chemistry, Delayed-Action Preparations, Muramidase, Sustained delivery, Coated Materials, Biocompatible / chemistry |
| Popis: | Since most of developed therapeutic proteins are intended to treat chronic diseases, patients are prescribed multiple injections for long time periods, and therefore, sustained release formulations are much needed. However, challenges facing these formulations are quite significant. In this context, a model protein, lysozyme (Lys), was loaded on hydrogel microparticles (beads) and the ability of layer-by-layer (LbL) coating to control Lys release and maintain its activity over a one-month period was investigated.LbL coating was composed of chondroitin sulfate as a negatively charged polyelectrolyte and a biocompatible, hydrolytically degradable poly β-aminoester as a positively charged polyelectrolyte. Loading distribution was monitored by fluorescence imaging, and followed by depositing a series of LbL coatings of different thicknesses. Release of Lys from these formulations was studied and activity of released fraction was determined.Lys was loaded effectively on hydrogel beads achieving about 9 mg protein/100 mg wet spheres. LbL coating was proven successful by monitoring the zeta potential of the beads, which was reversed after the addition of each layer. In vitro release studies showed sustained release profiles that depend on the thickness of the deposited coat, with t50 extended from 4.9 to 143.9 h. More importantly, released Lys possessed a high degree of biological activity during the course of release maintaining at least 72% of initial activity.Successful loading of Lys and extension of its release while maintaining a considerable degree of activity might make this formulation suitable for use with other active therapeutic proteins. |
| Druh dokumentu: | Article |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1521-0464 1071-7544 |
| DOI: | 10.3109/10717544.2015.1069422 |
| Přístupová URL adresa: | https://www.tandfonline.com/doi/pdf/10.3109/10717544.2015.1069422?needAccess=true https://pubmed.ncbi.nlm.nih.gov/26289209 https://www.tandfonline.com/doi/full/10.3109/10717544.2015.1069422 https://www.tandfonline.com/doi/pdf/10.3109/10717544.2015.1069422 https://pubmed.ncbi.nlm.nih.gov/26289209/ http://europepmc.org/abstract/MED/26250519 https://www.ncbi.nlm.nih.gov/pubmed/26250519 https://archive-ouverte.unige.ch/unige:182029 https://doi.org/10.3109/10717544.2015.1069422 |
| Přístupové číslo: | edsair.doi.dedup.....d13d5f8d90b09c7cfc6f93a38285ecd9 |
| Databáze: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | Since most of developed therapeutic proteins are intended to treat chronic diseases, patients are prescribed multiple injections for long time periods, and therefore, sustained release formulations are much needed. However, challenges facing these formulations are quite significant. In this context, a model protein, lysozyme (Lys), was loaded on hydrogel microparticles (beads) and the ability of layer-by-layer (LbL) coating to control Lys release and maintain its activity over a one-month period was investigated.LbL coating was composed of chondroitin sulfate as a negatively charged polyelectrolyte and a biocompatible, hydrolytically degradable poly β-aminoester as a positively charged polyelectrolyte. Loading distribution was monitored by fluorescence imaging, and followed by depositing a series of LbL coatings of different thicknesses. Release of Lys from these formulations was studied and activity of released fraction was determined.Lys was loaded effectively on hydrogel beads achieving about 9 mg protein/100 mg wet spheres. LbL coating was proven successful by monitoring the zeta potential of the beads, which was reversed after the addition of each layer. In vitro release studies showed sustained release profiles that depend on the thickness of the deposited coat, with t50 extended from 4.9 to 143.9 h. More importantly, released Lys possessed a high degree of biological activity during the course of release maintaining at least 72% of initial activity.Successful loading of Lys and extension of its release while maintaining a considerable degree of activity might make this formulation suitable for use with other active therapeutic proteins. |
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| ISSN: | 15210464 10717544 |
| DOI: | 10.3109/10717544.2015.1069422 |