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Cyanate and urea are substrates for nitrification by Thaumarchaeota in the marine environment

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Názov: Cyanate and urea are substrates for nitrification by Thaumarchaeota in the marine environment
Autori: Sten Littmann, Sandra Petrov, Michael Wagner, Craig W. Herbold, Maria Mooshammer, Katharina Kitzinger, Cory C. Padilla, Hannah K. Marchant, Laura A. Bristow, Marcel M. M. Kuypers, Martin Könneke, Frank J. Stewart, Philipp F. Hach, Abiel T. Kidane, Andreas Richter, Jutta Niggemann
Zdroj: Kitzinger, K, Padilla, C C, Marchant, H K, Hach, P F, Herbold, C W, Kidane, A T, Könneke, M, Littmann, S, Mooshammer, M, Niggemann, J, Petrov, S, Richter, A, Stewart, F J, Wagner, M, Kuypers, M M M & Bristow, L A 2019, ' Cyanate and urea are substrates for nitrification by Thaumarchaeota in the marine environment ', Nature Microbiology, vol. 4, no. 2, pp. 234-243 . https://doi.org/10.1038/s41564-018-0316-2
Informácie o vydavateľovi: Springer Science and Business Media LLC, 2018.
Rok vydania: 2018
Predmety: 0301 basic medicine, Seawater/chemistry, Nitrification/physiology, SDG 14 – Leben unter Wasser, GULF-OF-MEXICO, Ammonia/chemistry, MULTIPLE SEQUENCE ALIGNMENT, 03 medical and health sciences, Ammonia, TOOL, ARCHAEA, Urea, Seawater, SDG 14 - Life Below Water, 14. Life underwater, Cyanates, Nitrites, Phylogeny, AMMONIA OXIDATION-KINETICS, 106022 Mikrobiologie, Gulf of Mexico, 0303 health sciences, TARGETED OLIGONUCLEOTIDE PROBES, Nitrites/metabolism, NITRITE, Archaea, Nitrification, Archaea/classification, Oxygen/analysis, NITROGEN, Oxygen, SINGLE CELLS, BACTERIA, Cyanates/chemistry, 106022 Microbiology, Urea/chemistry, Energy Metabolism, Oxidation-Reduction
Popis: Ammonia-oxidizing archaea of the phylum Thaumarchaeota are among the most abundant marine microorganisms1. These organisms thrive in the oceans despite ammonium being present at low nanomolar concentrations2,3. Some Thaumarchaeota isolates have been shown to utilize urea and cyanate as energy and N sources through intracellular conversion to ammonium4-6. Yet, it is unclear whether patterns observed in culture extend to marine Thaumarchaeota, and whether Thaumarchaeota in the ocean directly utilize urea and cyanate or rely on co-occurring microorganisms to break these substrates down to ammonium. Urea utilization has been reported for marine ammonia-oxidizing communities7-10, but no evidence of cyanate utilization exists for marine ammonia oxidizers. Here, we demonstrate that in the Gulf of Mexico, Thaumarchaeota use urea and cyanate both directly and indirectly as energy and N sources. We observed substantial and linear rates of nitrite production from urea and cyanate additions, which often persisted even when ammonium was added to micromolar concentrations. Furthermore, single-cell analysis revealed that the Thaumarchaeota incorporated ammonium-, urea- and cyanate-derived N at significantly higher rates than most other microorganisms. Yet, no cyanases were detected in thaumarchaeal genomic data from the Gulf of Mexico. Therefore, we tested cyanate utilization in Nitrosopumilus maritimus, which also lacks a canonical cyanase, and showed that cyanate was oxidized to nitrite. Our findings demonstrate that marine Thaumarchaeota can use urea and cyanate as both an energy and N source. On the basis of these results, we hypothesize that urea and cyanate are substrates for ammonia-oxidizing Thaumarchaeota throughout the ocean.
Druh dokumentu: Article
Jazyk: English
ISSN: 2058-5276
DOI: 10.1038/s41564-018-0316-2
Prístupová URL adresa: https://europepmc.org/articles/pmc6825518?pdf=render
https://pubmed.ncbi.nlm.nih.gov/30531977
https://www.nature.com/articles/s41564-018-0316-2
http://publications.marum.de/3874/
https://europepmc.org/articles/PMC6825518/
https://pubmed.ncbi.nlm.nih.gov/30531977/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825518
http://www-nature-com-443.webvpn.bjmu.tsg211.com/articles/s41564-018-0316-2
https://portal.findresearcher.sdu.dk/da/publications/9cf1a597-6e9d-4262-bf5c-03e8600b064d
Rights: Springer Nature TDM
Prístupové číslo: edsair.doi.dedup.....cdfc4d325f4c2067b0f06e30677a3bba
Databáza: OpenAIRE
Popis
Abstrakt:Ammonia-oxidizing archaea of the phylum Thaumarchaeota are among the most abundant marine microorganisms1. These organisms thrive in the oceans despite ammonium being present at low nanomolar concentrations2,3. Some Thaumarchaeota isolates have been shown to utilize urea and cyanate as energy and N sources through intracellular conversion to ammonium4-6. Yet, it is unclear whether patterns observed in culture extend to marine Thaumarchaeota, and whether Thaumarchaeota in the ocean directly utilize urea and cyanate or rely on co-occurring microorganisms to break these substrates down to ammonium. Urea utilization has been reported for marine ammonia-oxidizing communities7-10, but no evidence of cyanate utilization exists for marine ammonia oxidizers. Here, we demonstrate that in the Gulf of Mexico, Thaumarchaeota use urea and cyanate both directly and indirectly as energy and N sources. We observed substantial and linear rates of nitrite production from urea and cyanate additions, which often persisted even when ammonium was added to micromolar concentrations. Furthermore, single-cell analysis revealed that the Thaumarchaeota incorporated ammonium-, urea- and cyanate-derived N at significantly higher rates than most other microorganisms. Yet, no cyanases were detected in thaumarchaeal genomic data from the Gulf of Mexico. Therefore, we tested cyanate utilization in Nitrosopumilus maritimus, which also lacks a canonical cyanase, and showed that cyanate was oxidized to nitrite. Our findings demonstrate that marine Thaumarchaeota can use urea and cyanate as both an energy and N source. On the basis of these results, we hypothesize that urea and cyanate are substrates for ammonia-oxidizing Thaumarchaeota throughout the ocean.
ISSN:20585276
DOI:10.1038/s41564-018-0316-2