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Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor

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Title: Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor
Authors: DongJoon Lee, Eun Jung Kim, Chengri Jin, Han Sung Jung, Jong Min Lee
Contributors: Dong-Joon Lee, Chengri Jin, Eun-Jung Kim, Jong-Min Lee, Han-Sung Jung, Kim, Eun Jung, Lee, Dong Joon, Jung, Han Sung
Source: Histochemistry and Cell Biology. 144:273-279
Publisher Information: Springer Science and Business Media LLC, 2015.
Publication Year: 2015
Subject Terms: Male, 0301 basic medicine, Ameloblasts/physiology, Cells, Genetic Vectors, Dental Enamel/metabolism, Incisor/growth & development, Calcification, Mice, 03 medical and health sciences, Calcification, Physiologic, Odontogenesis/genetics, Ameloblasts, Epithelial Cells/physiology, Ameloblast, Animals, Physiologic/genetics, Odontoblast, Dental Enamel, 10. No inequality, Inbred BALB C, Cells, Cultured, Mice, Inbred BALB C, Mice, Inbred ICR, 0303 health sciences, Cultured, Odontoblasts, Gastrin-releasing peptide (GRP), Dentin/metabolism, Epithelial Cells, Inbred ICR, Odontoblasts/physiology, 3. Good health, Incisor, Incisor development, Gastrin-Releasing Peptide, Enamel, Dentin, Odontogenesis, Gastrin-Releasing Peptide/genetics, Gastrin-Releasing Peptide/physiology
Description: Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide's receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.
Document Type: Article
Language: English
ISSN: 1432-119X
0948-6143
DOI: 10.1007/s00418-015-1335-1
Access URL: https://pubmed.ncbi.nlm.nih.gov/26126650
https://pubmed.ncbi.nlm.nih.gov/26126650/
https://link.springer.com/article/10.1007/s00418-015-1335-1
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140827
https://core.ac.uk/display/38070907
https://rd.springer.com/article/10.1007/s00418-015-1335-1
https://yonsei.pure.elsevier.com/en/publications/gastrin-releasing-peptide-expression-and-its-effect-on-the-calcif
http://hdl.handle.net/10722/211679
Rights: Springer TDM
CC BY NC ND
Accession Number: edsair.doi.dedup.....cd21f2ac9d7fb9ffdba5985ef3288c62
Database: OpenAIRE
Description
Abstract:Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide's receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.
ISSN:1432119X
09486143
DOI:10.1007/s00418-015-1335-1