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Comparison of antibiotic resistance and virulence between biofilm-producing and non-producing clinical isolates of Enterococcus faecium

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Title: Comparison of antibiotic resistance and virulence between biofilm-producing and non-producing clinical isolates of Enterococcus faecium
Authors: Anna Wieczorek, Piotr Wieczorek, Anna Sieńko, Elzbieta Tryniszewska, Piotr Majewski, Dorota Olszańska, Dominika Ojdana
Source: Acta Biochimica Polonica. 62:859-866
Publisher Information: Frontiers Media SA, 2015.
Publication Year: 2015
Subject Terms: 0301 basic medicine, Virulence, Enterococcus faecium - drug effects, Enterococcus faecium, Drug Resistance, Microbial, Microbial Sensitivity Tests, Microbial sensitivity tests, Anti-bacterial agents - pharmacology, Anti-Bacterial Agents, 3. Good health, 03 medical and health sciences, Genes, Drug resistance - microbial, Genes, Bacterial, Biofilms, Enterococcus faecium - pathogenicity, bacterial, Enterococcus faecium - genetics
Description: An increase in the antibiotic resistance among Enterococcus faecium strains has been observed worldwide. Moreover, this bacteria has the ability to produce several virulence factors and to form biofilm that plays an important role in human infections. This study was designed to compare the antibiotic resistance and the prevalence of genes encoding surface protein (esp), aggregation substance (as), surface adhesin (efaA), collagen adhesin (ace), gelatinase (gelE), and hialuronidase (hyl) between biofilm-producing and non-producing E. faecium strains. Therefore, ninety E. faecium clinical isolates were tested for biofilm-forming ability, and then were assigned to two groups: biofilm-positive (BIO(+), n =70) and biofilm-negative (BIO(-), n = 20). Comparison of these groups showed that BIO(+) isolates were resistant to β-lactams, whereas 10% of BIO(-) strains were susceptible to ampicillin (statistically significant difference, p = 0.007) and 5% to imipenem. Linezolid and tigecycline were the only antibiotics active against all tested isolates. Analysis of the virulence factors revealed that ace, efaA, and gelE genes occurred more frequently in BIO(-) strains (ace in 50% BIO(+) vs. 75% BIO(-); efaA 44.3% vs. 85%; gelE 2.9% vs. 15%, respectively), while hyl gene appeared more frequently in BIO(+) isolates (87.1% BIO(+) vs. 65% BIO(-)). These differences were significant (p < 0.05). We concluded that BIO(+) strains were more resistant to antibiotics than BIO(-) strains, but interestingly, BIO(-) isolates were characterized by possession of higher virulence capabilities.
Document Type: Article
Language: English
ISSN: 1734-154X
0001-527X
DOI: 10.18388/abp.2015_1147
Access URL: https://pubmed.ncbi.nlm.nih.gov/26637375
http://yadda.icm.edu.pl/yadda/element/bwmeta1.element.bwnjournal-article-abpv62p859kz
https://pubmed.ncbi.nlm.nih.gov/26637375/
https://ojs.ptbioch.edu.pl/index.php/abp/article/view/1725
http://europepmc.org/abstract/MED/26637375
https://www.ncbi.nlm.nih.gov/pubmed/26637375
Accession Number: edsair.doi.dedup.....c97ca3633cac95c2b47c9694b896e5bf
Database: OpenAIRE
Description
Abstract:An increase in the antibiotic resistance among Enterococcus faecium strains has been observed worldwide. Moreover, this bacteria has the ability to produce several virulence factors and to form biofilm that plays an important role in human infections. This study was designed to compare the antibiotic resistance and the prevalence of genes encoding surface protein (esp), aggregation substance (as), surface adhesin (efaA), collagen adhesin (ace), gelatinase (gelE), and hialuronidase (hyl) between biofilm-producing and non-producing E. faecium strains. Therefore, ninety E. faecium clinical isolates were tested for biofilm-forming ability, and then were assigned to two groups: biofilm-positive (BIO(+), n =70) and biofilm-negative (BIO(-), n = 20). Comparison of these groups showed that BIO(+) isolates were resistant to β-lactams, whereas 10% of BIO(-) strains were susceptible to ampicillin (statistically significant difference, p = 0.007) and 5% to imipenem. Linezolid and tigecycline were the only antibiotics active against all tested isolates. Analysis of the virulence factors revealed that ace, efaA, and gelE genes occurred more frequently in BIO(-) strains (ace in 50% BIO(+) vs. 75% BIO(-); efaA 44.3% vs. 85%; gelE 2.9% vs. 15%, respectively), while hyl gene appeared more frequently in BIO(+) isolates (87.1% BIO(+) vs. 65% BIO(-)). These differences were significant (p < 0.05). We concluded that BIO(+) strains were more resistant to antibiotics than BIO(-) strains, but interestingly, BIO(-) isolates were characterized by possession of higher virulence capabilities.
ISSN:1734154X
0001527X
DOI:10.18388/abp.2015_1147