Local myogenic pulp‐derived cell injection enhances craniofacial muscle regeneration in vivo
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| Názov: | Local myogenic pulp‐derived cell injection enhances craniofacial muscle regeneration in vivo |
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| Autori: | J. E. Jung, M. J. Song, S. Shin, Y. J. Choi, K. H. Kim, C. J. Chung |
| Prispievatelia: | College of Dentistry, Dept. of Orthodontics, J. E. Jung, M. J. Song, S. Shin, Y. J. Choi, K. H. Kim, C. J. Chung, Kim, Kyung Ho, Choi, Yoon Jeong, Shin, Su Jung, Chung, Choo Ryung |
| Zdroj: | Orthodontics & Craniofacial Research. 20:35-43 |
| Informácie o vydavateľovi: | Wiley, 2017. |
| Rok vydania: | 2017 |
| Predmety: | 5-aza-2′-deoxycytidine, 0301 basic medicine, Regeneration/drug effects, Adolescent, Cells, Cell Differentiation*/drug effects, Decitabine, Muscle Development, pulp cell, Young Adult, 03 medical and health sciences, Skeletal/physiology, muscle injury, Humans, Regeneration, Bicuspid, Muscle, Skeletal, Cells, Cultured, Dental Pulp, Masseter Muscle/physiology, 0303 health sciences, Cultured, Regeneration/physiology, Masseter Muscle, Cell Differentiation, DNA Methylation, Azacitidine/pharmacology, Azacitidine/analogs & derivatives, regeneration, Azacitidine, Muscle, Dental Pulp/cytology, Muscle Development/physiology, myogenesis |
| Popis: | Structured AbstractObjectivesTo enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5‐aza‐2′‐deoxycytidine (5‐Aza), and to evaluate the potent stimulatory effect of 5‐Aza‐treated pulp cell injection for craniofacial muscle regeneration in vivo.Setting and Sample PopulationPulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18‐22.1 years).Material and MethodsLevels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre‐treatment of 5‐Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)‐labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)‐induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice.ResultsPre‐treatment of 5‐Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5‐Aza pre‐treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles.ConclusionThe epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 1601-6343 1601-6335 |
| DOI: | 10.1111/ocr.12138 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/28102011 https://www.ncbi.nlm.nih.gov/pubmed/28102011 https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702266313373873 https://europepmc.org/article/MED/28102011 https://pubmed.ncbi.nlm.nih.gov/28102011/ https://ir.ymlib.yonsei.ac.kr/handle/22282913/154068 https://yonsei.pure.elsevier.com/en/publications/local-myogenic-pulp-derived-cell-injection-enhances-craniofacial- |
| Rights: | Wiley Online Library User Agreement CC BY NC ND |
| Prístupové číslo: | edsair.doi.dedup.....c8ed30f8b87dd819b67b802cd42053e9 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Structured AbstractObjectivesTo enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5‐aza‐2′‐deoxycytidine (5‐Aza), and to evaluate the potent stimulatory effect of 5‐Aza‐treated pulp cell injection for craniofacial muscle regeneration in vivo.Setting and Sample PopulationPulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18‐22.1 years).Material and MethodsLevels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre‐treatment of 5‐Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)‐labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)‐induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice.ResultsPre‐treatment of 5‐Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5‐Aza pre‐treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles.ConclusionThe epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo. |
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| ISSN: | 16016343 16016335 |
| DOI: | 10.1111/ocr.12138 |