In EDS ansehen

Distribution and volume of mitochondria in alveolar epithelial type 1 cells in infant and adult human lungs

Gespeichert in:
Bibliographische Detailangaben
Titel: Distribution and volume of mitochondria in alveolar epithelial type 1 cells in infant and adult human lungs
Autoren: Schierz, Arne K., Rößler, Giacomo, Schneider, Jan Philipp, Tschanz, Stefan A., Werlein, Christopher, Jonigk, Danny D., Schipke, Julia, Mühlfeld, Christian
Quelle: Histochem Cell Biol
Verlagsinformationen: Springer Science and Business Media LLC, 2024.
Publikationsjahr: 2024
Schlagwörter: Adult, Pulmonary Alveoli, Male, Original Paper, Young Adult, Female [MeSH], Mitochondria, Development, Mitochondria/metabolism [MeSH], Adult [MeSH], Humans [MeSH], Pulmonary Alveoli/cytology [MeSH], Lung/metabolism [MeSH], Alveolar epithelial type 1 cells, Infant [MeSH], Male [MeSH], Alveolar Epithelial Cells/cytology [MeSH], Pulmonary Alveoli/metabolism [MeSH], Young Adult [MeSH], Alveolar Epithelial Cells/metabolism [MeSH], Alveolar epithelium, Lung/cytology [MeSH], Stereology, Alveolar Epithelial Cells, Humans, Infant, Female, Lung
Beschreibung: Alveolar epithelial type I (AE1) cells with their wide spatial expansion form approximately 95% of the outer surface area of the air-blood barrier inside the lung. Serial block-face scanning electron microscopy (SBF-SEM) investigations led to the hypothesis that AE1 cell mitochondria are preferentially distributed as aggregates in those parts of AE1 cells that are located above connective tissue pillars between capillaries, thus not increasing the thickness of the diffusion distance for oxygen and carbon dioxide. Furthermore, it was hypothesised that postnatal development requires adapting the amount and distribution of mitochondria in AE1 cells. Human lung samples from three infant (26 and 30 days, 6 months) and three adult (20, 39 and 40 years) samples were investigated by light microscopy, transmission electron microscopy and stereology. The volume fraction of mitochondria was similar in infant and adult lungs with a mean value of 6.3%. The ratio between mitochondrial profiles on top of capillaries or above connective tissue pillars was approximately 3:1 in infants and adults. However, regarding the volume of both cytoplasmic compartments, infants showed a higher number of mitochondrial profiles on top of capillaries while adults showed a higher number above connective tissue pillars. Samples of three additional adult lungs were analysed by confocal laser scanning microscopy. Again, mitochondria were not preferentially found as aggregates above connective tissue pillars. In conclusion, AE1 cell mitochondria were not preferentially found as aggregates, showed the same volume density in infants and adults but differed in distribution between the age groups.
Publikationsart: Article
Other literature type
Sprache: English
ISSN: 1432-119X
0948-6143
DOI: 10.1007/s00418-024-02332-7
DOI: 10.48620/77021
Zugangs-URL: https://pubmed.ncbi.nlm.nih.gov/39557665
https://repository.publisso.de/resource/frl:6520147
Rights: CC BY
Dokumentencode: edsair.doi.dedup.....c84d1d27a9468cd02ed928ff60ef0dc5
Datenbank: OpenAIRE
Beschreibung
Abstract:Alveolar epithelial type I (AE1) cells with their wide spatial expansion form approximately 95% of the outer surface area of the air-blood barrier inside the lung. Serial block-face scanning electron microscopy (SBF-SEM) investigations led to the hypothesis that AE1 cell mitochondria are preferentially distributed as aggregates in those parts of AE1 cells that are located above connective tissue pillars between capillaries, thus not increasing the thickness of the diffusion distance for oxygen and carbon dioxide. Furthermore, it was hypothesised that postnatal development requires adapting the amount and distribution of mitochondria in AE1 cells. Human lung samples from three infant (26 and 30 days, 6 months) and three adult (20, 39 and 40 years) samples were investigated by light microscopy, transmission electron microscopy and stereology. The volume fraction of mitochondria was similar in infant and adult lungs with a mean value of 6.3%. The ratio between mitochondrial profiles on top of capillaries or above connective tissue pillars was approximately 3:1 in infants and adults. However, regarding the volume of both cytoplasmic compartments, infants showed a higher number of mitochondrial profiles on top of capillaries while adults showed a higher number above connective tissue pillars. Samples of three additional adult lungs were analysed by confocal laser scanning microscopy. Again, mitochondria were not preferentially found as aggregates above connective tissue pillars. In conclusion, AE1 cell mitochondria were not preferentially found as aggregates, showed the same volume density in infants and adults but differed in distribution between the age groups.
ISSN:1432119X
09486143
DOI:10.1007/s00418-024-02332-7