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Periodontitis mainly increases osteoclast formation via enhancing the differentiation of quiescent osteoclast precursors into osteoclasts

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Název: Periodontitis mainly increases osteoclast formation via enhancing the differentiation of quiescent osteoclast precursors into osteoclasts
Autoři: Yun-Jung Yoo, Eun Jung Bak, Dong Eun Lee, Seong-Ho Choi, Jeong Heon Cha, Jin Kim
Přispěvatelé: D E Lee, J H Kim, S H Choi, J H Cha, E J Bak, Y J Yoo, Kim, Ji Hye, Bak, Eun-Jung, Yoo, Yun Jung, Lee, Dong Eun, Cha, Jung Heon, Choi, Seong Ho
Zdroj: Journal of Periodontal Research. 50:256-264
Informace o vydavateli: Wiley, 2014.
Rok vydání: 2014
Témata: Tartrate-Resistant Acid Phosphatase/analysis, Male, Alveolar Process/pathology, Time Factors, Neutrophils, Alveolar Bone Loss, Osteoclasts, Cell Count, Osteoclasts/physiology, Tooth Cervix, Cell Nucleus/pathology, Leukocyte Count, 03 medical and health sciences, 0302 clinical medicine, Leukocytes, quiescent osteoclast precursors, Alveolar Process, Cell Differentiation/physiology, Periodontitis/pathology, Animals, Periodontitis, 10. No inequality, periodontitis, Neutrophils/pathology, Cell Nucleus, Inbred F344, Mononuclear/pathology, Tartrate-Resistant Acid Phosphatase, Stem Cells, Cell Differentiation, differentiation, Rats, Inbred F344, 6. Clean water, Rats, Stem Cells/physiology, Osteoclasts/pathology, osteoclasts, Bromodeoxyuridine, Leukocytes, Mononuclear, Alveolar Bone Loss/pathology, Tooth Cervix/pathology
Popis: Background and objectivesTartrate‐resistant acid phosphatase (TRAP)‐positive multinucleated osteoclasts are formed in sequential steps: proliferation and differentiation of hematopoietic progenitors into quiescent osteoclast precursors (QOPs), followed by fusion of QOPs. In this study, we investigated whether enhancement of osteoclast formation by periodontitis is derived from the stimulation of proliferation of hematopoietic progenitors or the differentiation of QOPs into osteoclasts.Material and methodsLigatures were placed around the first molars in the left mandibles of Fischer 344 inbred rats. The rats received drinking water containing bromodeoxyuridine (BrdU) (which can be incorporated into dividing nuclei) after ligation during the experimental period. The number of inflammatory cells in the distal area was counted. Alveolar bone loss was histologically estimated by measuring the distance from the cementoenamel junction to the alveolar bone crest in the distal area and determining the percentage of periodontal ligament area in the furcation. The number of osteoclasts and percentage of BrdU+ nuclei in total osteoclasts nuclei were counted after TRAP and BrdU double labeling.ResultsThe number of polymorphonuclear cells increased on day 1 and then rapidly decreased. The number of mononuclear cells increased in a time‐dependent manner up to day 5 and remained the same until day 10. Alveolar bone loss of ligatured teeth increased in a time‐dependent manner. The number of osteoclasts peaked on day 3 then gradually decreased. At peak, the percentage of BrdU+ nuclei in total osteoclasts nuclei in the distal and furcation areas were 7.9% and 4.1%, respectively.ConclusionThese results indicate that most of the osteoclasts formed after periodontitis induction are derived from preformed QOPs, suggesting that enhancement of osteoclast formation by periodontitis might be mainly caused by stimulating the differentiation of QOPs into osteoclasts.
Druh dokumentu: Article
Popis souboru: 256~264
Jazyk: English
ISSN: 1600-0765
0022-3484
DOI: 10.1111/jre.12203
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/25039828
https://europepmc.org/article/MED/25039828
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139168
https://yonsei.pure.elsevier.com/en/publications/periodontitis-mainly-increases-osteoclast-formation-via-enhancing
https://www.ncbi.nlm.nih.gov/pubmed/25039828
https://onlinelibrary.wiley.com/doi/10.1111/jre.12203
Rights: Wiley Online Library User Agreement
CC BY NC ND
Přístupové číslo: edsair.doi.dedup.....c7e4191357632446cc75382d2c1a7d32
Databáze: OpenAIRE
Popis
Abstrakt:Background and objectivesTartrate‐resistant acid phosphatase (TRAP)‐positive multinucleated osteoclasts are formed in sequential steps: proliferation and differentiation of hematopoietic progenitors into quiescent osteoclast precursors (QOPs), followed by fusion of QOPs. In this study, we investigated whether enhancement of osteoclast formation by periodontitis is derived from the stimulation of proliferation of hematopoietic progenitors or the differentiation of QOPs into osteoclasts.Material and methodsLigatures were placed around the first molars in the left mandibles of Fischer 344 inbred rats. The rats received drinking water containing bromodeoxyuridine (BrdU) (which can be incorporated into dividing nuclei) after ligation during the experimental period. The number of inflammatory cells in the distal area was counted. Alveolar bone loss was histologically estimated by measuring the distance from the cementoenamel junction to the alveolar bone crest in the distal area and determining the percentage of periodontal ligament area in the furcation. The number of osteoclasts and percentage of BrdU+ nuclei in total osteoclasts nuclei were counted after TRAP and BrdU double labeling.ResultsThe number of polymorphonuclear cells increased on day 1 and then rapidly decreased. The number of mononuclear cells increased in a time‐dependent manner up to day 5 and remained the same until day 10. Alveolar bone loss of ligatured teeth increased in a time‐dependent manner. The number of osteoclasts peaked on day 3 then gradually decreased. At peak, the percentage of BrdU+ nuclei in total osteoclasts nuclei in the distal and furcation areas were 7.9% and 4.1%, respectively.ConclusionThese results indicate that most of the osteoclasts formed after periodontitis induction are derived from preformed QOPs, suggesting that enhancement of osteoclast formation by periodontitis might be mainly caused by stimulating the differentiation of QOPs into osteoclasts.
ISSN:16000765
00223484
DOI:10.1111/jre.12203