cDNA cloning and deduced amino acid sequence of a major, glycine‐rich cuticular protein from the coleopteran Tenebrio molitor: Temporal and spatial distribution of the transcript during metamorphosis
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| Title: | cDNA cloning and deduced amino acid sequence of a major, glycine‐rich cuticular protein from the coleopteran Tenebrio molitor: Temporal and spatial distribution of the transcript during metamorphosis |
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| Authors: | Charles, Jean-Philippe, Bouhin, Hervé, Quennedey, Brigitte, Courrent, Annie, Delachambre, Jean |
| Contributors: | Delon, Viviane |
| Source: | European Journal of Biochemistry. 206:813-819 |
| Publisher Information: | Wiley, 1992. |
| Publication Year: | 1992 |
| Subject Terms: | Electrophoresis, 0301 basic medicine, Molecular Sequence Data, Glycine, Proteins/chemistry/*genetics, DNA/chemistry/*genetics, 03 medical and health sciences, Biological/genetics/physiology, Animals, Northern, Electrophoresis, Gel, Two-Dimensional, Amino Acid Sequence, Cloning, Molecular, Tenebrio, Gel, 0303 health sciences, Metamorphosis, Base Sequence, Blotting, Metamorphosis, Biological, Molecular, Nucleic Acid Hybridization, Proteins, DNA, Blotting, Northern, Tenebrio/chemistry/*genetics, Gene Expression Regulation, Glycine/analysis, Two-Dimensional, Insect Proteins, Cloning |
| Description: | In Coleoptera, the elytra (forewings), with a very hard and thick cuticle, protect the membranous and delicate hindwings against mechanical stress. We have isolated and characterized a cDNA encoding a major cuticle protein in Tenebrio molitor, named ACP‐20. The deduced amino acid sequence is roughly tripartite, with two terminal glycine‐rich domains and a central region showing pronounced similarities with some other hard cuticle proteins. Northern blot and in situ hybridization analyses reveal that ACP‐20 gene expression is developmentally regulated since transcript accumulation occurs only in epidermal regions synthesizing hard cuticle and is restricted to the period of preecdysial adult cuticle deposition. Moreover, application of a juvenile hormone analogue prevents the appearance of the transcript, indicating that juvenile hormone, a key molecule involved in the control of insect metamorphosis, negatively regulates the expression of the ACP‐20 gene. |
| Document Type: | Article Other literature type |
| Language: | English |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1992.tb16989.x |
| Access URL: | https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1432-1033.1992.tb16989.x https://pubmed.ncbi.nlm.nih.gov/1606964 https://dumas.ccsd.cnrs.fr/CSGA/hal-00451540 https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1432-1033.1992.tb16989.x https://febs.onlinelibrary.wiley.com/doi/full/10.1111/j.1432-1033.1992.tb16989.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1992.tb16989.x http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1992.tb16989.x/abstract http://www.ncbi.nlm.nih.gov/pubmed/1606964 https://hal.science/hal-00451540v1 |
| Rights: | Wiley Online Library User Agreement |
| Accession Number: | edsair.doi.dedup.....c5b296eb1ebc27dd50a82b40eab9d7d9 |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | In Coleoptera, the elytra (forewings), with a very hard and thick cuticle, protect the membranous and delicate hindwings against mechanical stress. We have isolated and characterized a cDNA encoding a major cuticle protein in Tenebrio molitor, named ACP‐20. The deduced amino acid sequence is roughly tripartite, with two terminal glycine‐rich domains and a central region showing pronounced similarities with some other hard cuticle proteins. Northern blot and in situ hybridization analyses reveal that ACP‐20 gene expression is developmentally regulated since transcript accumulation occurs only in epidermal regions synthesizing hard cuticle and is restricted to the period of preecdysial adult cuticle deposition. Moreover, application of a juvenile hormone analogue prevents the appearance of the transcript, indicating that juvenile hormone, a key molecule involved in the control of insect metamorphosis, negatively regulates the expression of the ACP‐20 gene. |
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| ISSN: | 14321033 00142956 |
| DOI: | 10.1111/j.1432-1033.1992.tb16989.x |