Zobrazit v EDS

Aβ pathology downregulates brain mGluR5 density in a mouse model of Alzheimer

Uloženo v:
Podrobná bibliografie
Název: Aβ pathology downregulates brain mGluR5 density in a mouse model of Alzheimer
Autoři: Minkyung Lee, Hae-June Lee, In Suh Park, Ji-Ae Park, Yeon Ju Kwon, Young Hoon Ryu, Chul Hoon Kim, Joo Hyun Kang, In Young Hyun, Kyo Chul Lee, Jae Yong Choi
Přispěvatelé: Minkyung Lee, Hae-June Lee, In Suh Park, Ji-Ae Park, Yeon Ju Kwon, Young Hoon Ryu, Chul Hoon Kim, Joo Hyun Kang, In Young Hyun, Kyo Chul Lee, Jae Yong Choi, Kim, Chul Hoon
Zdroj: Neuropharmacology. 133:512-517
Informace o vydavateli: Elsevier BV, 2018.
Rok vydání: 2018
Témata: Male, 0301 basic medicine, Amyloid, Mutation / genetics, Metabotropic Glutamate 5 / metabolism, Pyridines, Image Processing, Presenilin-1 / genetics, Receptor, Metabotropic Glutamate 5, Brain / diagnostic imaging, Amyloid beta-Protein Precursor / genetics, Down-Regulation, Mice, Transgenic, Pyridines / pharmacokinetics, Down-Regulation / drug effects, Transgenic, Amyloid beta-Protein Precursor, Mice, 03 medical and health sciences, Computer-Assisted, 0302 clinical medicine, Alzheimer Disease, Nitriles, Image Processing, Computer-Assisted, Presenilin-1, Animals, Alzheimer Disease / pathology, Down-Regulation / genetics, Amyloid beta-Peptides, Amyloid beta-Peptides / metabolism, Animal, Nitriles / pharmacokinetics, Brain, Brain / metabolism, Magnetic Resonance Imaging, Peptide Fragments, Amyloid / metabolism, Alzheimer Disease / genetics, Disease Models, Animal, Positron-Emission Tomography, Disease Models, Mutation, Alzheimer Disease / diagnostic imaging, Peptide Fragments / metabolism, Receptor
Popis: The aim of the present study was to evaluate functional changes of mGluR5 expression in advanced Alzheimer's disease (AD) using positron emission tomography (PET) with an mGluR5 specific radiotracer ([18F]FPEB) in 5xFAD AD model. Subsequently, in the same animal, mGluR5 expression was quantified by immunoassay techniques. The non-displaceable binding potential values for mGluR5 was estimated by the Logan's graphical analysis. Brain PET imaging revealed that radioactivities in the hippocampus and the striatum were significantly lower in 5xFAD mice compared to control animals. Binding values were also significantly lowered in 5xFAD mice. This decline was validated by immunoblotting of protein isolates from brain tissues, as the mean band density for 5xFAD mice had a lower mGluR5 intensity than for wild type mice. These results indicated that mGluR5 levels in 5xFAD mice were down regulated in the limbic system.
Druh dokumentu: Article
Jazyk: English
ISSN: 0028-3908
DOI: 10.1016/j.neuropharm.2018.02.003
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/29427650
https://www.sciencedirect.com/science/article/pii/S0028390818300601
https://pubmed.ncbi.nlm.nih.gov/29427650/
https://www.ncbi.nlm.nih.gov/pubmed/29427650
http://europepmc.org/abstract/MED/29427650
Rights: Elsevier TDM
CC BY NC ND
Přístupové číslo: edsair.doi.dedup.....c2dfc9683df89c589a15d627d5c6d2aa
Databáze: OpenAIRE
Popis
Abstrakt:The aim of the present study was to evaluate functional changes of mGluR5 expression in advanced Alzheimer's disease (AD) using positron emission tomography (PET) with an mGluR5 specific radiotracer ([18F]FPEB) in 5xFAD AD model. Subsequently, in the same animal, mGluR5 expression was quantified by immunoassay techniques. The non-displaceable binding potential values for mGluR5 was estimated by the Logan's graphical analysis. Brain PET imaging revealed that radioactivities in the hippocampus and the striatum were significantly lower in 5xFAD mice compared to control animals. Binding values were also significantly lowered in 5xFAD mice. This decline was validated by immunoblotting of protein isolates from brain tissues, as the mean band density for 5xFAD mice had a lower mGluR5 intensity than for wild type mice. These results indicated that mGluR5 levels in 5xFAD mice were down regulated in the limbic system.
ISSN:00283908
DOI:10.1016/j.neuropharm.2018.02.003