Functional and molecular features of the Id4+ germline stem cell population in mouse testes
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| Název: | Functional and molecular features of the Id4+ germline stem cell population in mouse testes |
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| Autoři: | Chan, Frieda, Oatley, Melissa J, Kaucher, Amy V, Yang, Qi-En, Bieberich, Charles J, Shashikant, Cooduvalli S, Oatley, Jon M |
| Zdroj: | Genes Dev |
| Informace o vydavateli: | Cold Spring Harbor Laboratory, 2014. |
| Rok vydání: | 2014 |
| Témata: | Male, 0301 basic medicine, Green Fluorescent Proteins, Spermatogenesis - genetics, Germ Cells - metabolism, Mice, Transgenic, Stem Cells - cytology, Transgenic, Mice, 03 medical and health sciences, Inhibitor of Differentiation Proteins - genetics, Testis, Animals, Developmental, Testis - cytology, Spermatogenesis, Germ Cells - cytology, 2. Zero hunger, Inhibitor of Differentiation Proteins - metabolism, 0303 health sciences, Green Fluorescent Proteins - genetics, Green Fluorescent Proteins - metabolism, Stem Cells, Gene Expression Regulation, Developmental, Cell Differentiation, Stem Cells - metabolism, Spermatogonia, Germ Cells, Gene Expression Regulation, Testis - metabolism, Inhibitor of Differentiation Proteins, Spermatogonia - metabolism, Transcriptome, Research Paper |
| Popis: | The maintenance of cycling cell lineages relies on undifferentiated subpopulations consisting of stem and progenitor pools. Features that delineate these cell types are undefined for many lineages, including spermatogenesis, which is supported by an undifferentiated spermatogonial population. Here, we generated a transgenic mouse line in which spermatogonial stem cells are marked by expression of an inhibitor of differentiation 4 (Id4)-green fluorescent protein (Gfp) transgene. We found that Id4-Gfp+ cells exist primarily as a subset of the type Asingle pool, and their frequency is greatest in neonatal development and then decreases in proportion during establishment of the spermatogenic lineage, eventually comprising ∼2% of the undifferentiated spermatogonial population in adulthood. RNA sequencing analysis revealed that expression of 11 and 25 genes is unique for the Id4-Gfp+/stem cell and Id4-Gfp−/progenitor fractions, respectively. Collectively, these findings provide the first definitive evidence that stem cells exist as a rare subset of the Asingle pool and reveal transcriptome features distinguishing stem cell and progenitor states within the mammalian male germline. |
| Druh dokumentu: | Article Other literature type |
| Jazyk: | English |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.240465.114 |
| Přístupová URL adresa: | http://genesdev.cshlp.org/content/28/12/1351.full.pdf https://pubmed.ncbi.nlm.nih.gov/24939937 https://pubmed.ncbi.nlm.nih.gov/24939937/ https://pennstate.pure.elsevier.com/en/publications/functional-and-molecular-features-of-the-id4supsup-germline-stem- http://genesdev.cshlp.org/content/28/12/1351.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24939937/ https://pubpeer.com/publications/A1A3726C85DBD4E01CC84DAAD74FB0 https://www.ncbi.nlm.nih.gov/pubmed/24939937 |
| Rights: | CC BY NC |
| Přístupové číslo: | edsair.doi.dedup.....c0eeac576cc3e87569957a41ecf6c21f |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The maintenance of cycling cell lineages relies on undifferentiated subpopulations consisting of stem and progenitor pools. Features that delineate these cell types are undefined for many lineages, including spermatogenesis, which is supported by an undifferentiated spermatogonial population. Here, we generated a transgenic mouse line in which spermatogonial stem cells are marked by expression of an inhibitor of differentiation 4 (Id4)-green fluorescent protein (Gfp) transgene. We found that Id4-Gfp+ cells exist primarily as a subset of the type Asingle pool, and their frequency is greatest in neonatal development and then decreases in proportion during establishment of the spermatogenic lineage, eventually comprising ∼2% of the undifferentiated spermatogonial population in adulthood. RNA sequencing analysis revealed that expression of 11 and 25 genes is unique for the Id4-Gfp+/stem cell and Id4-Gfp−/progenitor fractions, respectively. Collectively, these findings provide the first definitive evidence that stem cells exist as a rare subset of the Asingle pool and reveal transcriptome features distinguishing stem cell and progenitor states within the mammalian male germline. |
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| ISSN: | 15495477 08909369 |
| DOI: | 10.1101/gad.240465.114 |