Performance of three chromogenic VRE screening agars, two Etest® vancomycin protocols, and different microdilution methods in detecting vanB genotype Enterococcus faecium with varying vancomycin MICs
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| Název: | Performance of three chromogenic VRE screening agars, two Etest® vancomycin protocols, and different microdilution methods in detecting vanB genotype Enterococcus faecium with varying vancomycin MICs |
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| Autoři: | Klare, Ingo, Fleige, Carola, Geringer, Uta, Witte, Wolfgang, Werner, Guido |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 74:171-176 |
| Informace o vydavateli: | Elsevier BV, 2012. |
| Rok vydání: | 2012 |
| Témata: | 0301 basic medicine, Genotype, Enterococcus faecium, 610 Medizin, Microbial Sensitivity Tests, Sensitivity and Specificity, Chromogenic Compounds/metabolism, 03 medical and health sciences, Mass Screening/methods, Bacterial Proteins, Humans, Mass Screening, Gram-Positive Bacterial Infections, ddc:610, 0303 health sciences, Bacterial Proteins/genetics, Enterococcus faecium/genetics, Enterococcus faecium/growth & development, Vancomycin Resistance, Gram-Positive Bacterial Infections/diagnosis, Culture Media, 3. Good health, Europe, Agar, Chromogenic Compounds, Enterococcus faecium/isolation & purification, Microbial Sensitivity Tests/methods, Enterococcus faecium/drug effects, Culture Media/chemistry |
| Popis: | Frequencies of vanB-type Enterococcus faecium increased in Europe during the last years. VanB enterococci show various levels of vancomycin MICs even below the susceptible breakpoint challenging a reliable diagnostics. The performance of 3 chromogenic vancomycin-resistant enterococci (VRE) screening agars, 2 Etest® vancomycin protocols, and different microdilution methods to detect 129 clinical vanB E. faecium strains was investigated. Altogether, 112 (87%) were correctly identified as VanB-type Enterococcus by microdilution MICs. An Etest® macromethod protocol was more sensitive than the standard protocol while keeping sufficient specificity in identifying 15 vanA/vanB-negative strains. Three chromogenic VRE agars performed similarly with 121 (94%), 123 (95%), and 124 (96%) vanB isolates that grew on Brilliance™ VRE Agar, CHROMagar™ VRE, and chromID™ VRE agar, respectively. Using identical media and conditions, we did not identify different growth behaviour on agar and in broth. A few vanB strains showed growth of microcolonies inside the Etest® vancomycin inhibition zones, suggesting a VanB heteroresistance phenotype. |
| Druh dokumentu: | Article |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2012.06.020 |
| DOI: | 10.25646/1607 |
| Přístupová URL adresa: | https://edoc.rki.de/bitstream/176904/1682/1/22eUdsZN2QLGQ.pdf https://pubmed.ncbi.nlm.nih.gov/22901792 https://edoc.rki.de/handle/176904/1682 https://edoc.rki.de/bitstream/176904/1682/1/22eUdsZN2QLGQ.pdf https://europepmc.org/article/MED/22901792 http://www.ncbi.nlm.nih.gov/pubmed/22901792 https://www.sciencedirect.com/science/article/pii/S0732889312002714 http://www.sciencedirect.com/science/article/pii/S0732889312002714 |
| Rights: | Elsevier TDM |
| Přístupové číslo: | edsair.doi.dedup.....bf1f54b863414c8507ae4648cf178c2a |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Frequencies of vanB-type Enterococcus faecium increased in Europe during the last years. VanB enterococci show various levels of vancomycin MICs even below the susceptible breakpoint challenging a reliable diagnostics. The performance of 3 chromogenic vancomycin-resistant enterococci (VRE) screening agars, 2 Etest® vancomycin protocols, and different microdilution methods to detect 129 clinical vanB E. faecium strains was investigated. Altogether, 112 (87%) were correctly identified as VanB-type Enterococcus by microdilution MICs. An Etest® macromethod protocol was more sensitive than the standard protocol while keeping sufficient specificity in identifying 15 vanA/vanB-negative strains. Three chromogenic VRE agars performed similarly with 121 (94%), 123 (95%), and 124 (96%) vanB isolates that grew on Brilliance™ VRE Agar, CHROMagar™ VRE, and chromID™ VRE agar, respectively. Using identical media and conditions, we did not identify different growth behaviour on agar and in broth. A few vanB strains showed growth of microcolonies inside the Etest® vancomycin inhibition zones, suggesting a VanB heteroresistance phenotype. |
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| ISSN: | 07328893 |
| DOI: | 10.1016/j.diagmicrobio.2012.06.020 |