Zobrazit v EDS

Virulence of Klebsiella pneumoniae Isolates Harboring blaKPC-2 Carbapenemase Gene in a Caenorhabditis elegans Model

Uloženo v:
Podrobná bibliografie
Název: Virulence of Klebsiella pneumoniae Isolates Harboring blaKPC-2 Carbapenemase Gene in a Caenorhabditis elegans Model
Autoři: Lavigne, Jean-Philippe, Cuzon, Gaelle, Combescure, Christophe, Bourg, Gisèle, Sotto, Albert, Nordmann, Patrice
Zdroj: PLoS One
PLoS ONE, Vol 8, Iss 7, p e67847 (2013)
PLOS ONE, Vol. 8, No 7 (2013) P. e67847
Informace o vydavateli: Public Library of Science (PLoS), 2013.
Rok vydání: 2013
Témata: 0301 basic medicine, Time Factors, Science, Microbial Sensitivity Tests, Klebsiella pneumoniae/enzymology/pathogenicity/physiology, beta-Lactamases, Anti-Bacterial Agents/pharmacology, Virulence/genetics, 03 medical and health sciences, Bacterial Proteins, Species Specificity, Animals, Caenorhabditis elegans, ddc:613, Microbial Viability, Virulence, Microbial Viability/drug effects, Plasmids/genetics/metabolism, Beta-Lactamases/genetics/metabolism, Anti-Bacterial Agents, 3. Good health, Klebsiella pneumoniae, Host-Pathogen Interactions, Bacterial Proteins/genetics/metabolism, Caenorhabditis elegans/growth & development/microbiology, Medicine, Research Article, Plasmids
Popis: Klebsiella pneumoniae carbapenemase (KPC) is a carbapenemase increasingly reported worldwide in Enterobacteriaceae. The aim of this study was to analyze the virulence of several KPC-2-producing K. pneumoniae isolates. The studied strains were (i) five KPC-2 clinical strains from different geographical origins, belonging to different ST-types and possessing plasmids of different incompatibility groups; (ii) seven transformants obtained after electroporation of either these natural KPC plasmids or a recombinant plasmid harboring only the bla KPC-2 gene into reference strains K. pneumoniae ATCC10031/CIP53153; and (iii) five clinical strains cured of plasmids. The virulence of K. pneumoniae isolates was evaluated in the Caenorhabditis elegans model. The clinical KPC producers and transformants were significantly less virulent (LT50: 5.5 days) than K. pneumoniae reference strain (LT50: 4.3 days) (p
Druh dokumentu: Article
Other literature type
Popis souboru: application/pdf
Jazyk: English
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0067847
Přístupová URL adresa: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0067847&type=printable
https://pubmed.ncbi.nlm.nih.gov/23844109
https://doaj.org/article/0a1f4a7a0dc842c7a25b62280e7c0f46
https://archive-ouverte.unige.ch/unige:42548
https://core.ac.uk/display/60914721
http://www.ncbi.nlm.nih.gov/pubmed/23844109
https://pubmed.ncbi.nlm.nih.gov/23844109/
http://europepmc.org/articles/PMC3699468
https://ui.adsabs.harvard.edu/abs/2013PLoSO...867847L/abstract
https://archive-ouverte.unige.ch/unige:42548
https://doi.org/10.1371/journal.pone.0067847
https://archive-ouverte.unige.ch/unige:42548
Rights: CC BY
Přístupové číslo: edsair.doi.dedup.....bd07fd8dd8a72882503b286a2b9001ed
Databáze: OpenAIRE
Popis
Abstrakt:Klebsiella pneumoniae carbapenemase (KPC) is a carbapenemase increasingly reported worldwide in Enterobacteriaceae. The aim of this study was to analyze the virulence of several KPC-2-producing K. pneumoniae isolates. The studied strains were (i) five KPC-2 clinical strains from different geographical origins, belonging to different ST-types and possessing plasmids of different incompatibility groups; (ii) seven transformants obtained after electroporation of either these natural KPC plasmids or a recombinant plasmid harboring only the bla KPC-2 gene into reference strains K. pneumoniae ATCC10031/CIP53153; and (iii) five clinical strains cured of plasmids. The virulence of K. pneumoniae isolates was evaluated in the Caenorhabditis elegans model. The clinical KPC producers and transformants were significantly less virulent (LT50: 5.5 days) than K. pneumoniae reference strain (LT50: 4.3 days) (p
ISSN:19326203
DOI:10.1371/journal.pone.0067847