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Pharmaceutical Agents Known to Produce Disulfiram-Like Reaction: Effects on Hepatic Ethanol Metabolism and Brain Monoamines

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Title: Pharmaceutical Agents Known to Produce Disulfiram-Like Reaction: Effects on Hepatic Ethanol Metabolism and Brain Monoamines
Authors: Karamanakos, P. N., Pappas, P., Boumba, V. A., Thomas, C., Malamas, M., Vougiouklakis, T., Marselos, M.
Source: International Journal of Toxicology. 26:423-432
Publisher Information: SAGE Publications, 2007.
Publication Year: 2007
Subject Terms: Male, 0301 basic medicine, Mesencephalon/chemistry/drug effects/metabolism, Ethanol/*metabolism, Biogenic Monoamines/*metabolism, Dopamine, Norepinephrine/metabolism, Hypothalamus, Furazolidone/administration & dosage/pharmacology, Acetaldehyde, Dopamine beta-Hydroxylase, 3,4-Dihydroxyphenylacetic Acid/metabolism, Serotonin/metabolism, Acetaldehyde/metabolism, Alcohol Dehydrogenase/metabolism, 03 medical and health sciences, 0302 clinical medicine, Mesencephalon, Metronidazole, Disulfiram, Animals, Biogenic Monoamines, Rats, Wistar, Chloramphenicol/administration & dosage/pharmacology, Dopamine beta-Hydroxylase/antagonists & inhibitors, Hypothalamus/chemistry/drug effects/metabolism, Intubation, Gastrointestinal, Ethanol, Aldehyde Dehydrogenase/metabolism, Alcohol Dehydrogenase, Furazolidone, Metronidazole/administration & dosage/pharmacology, Homovanillic Acid, Disulfiram/administration & dosage/*pharmacology/standards, Homovanillic Acid/metabolism, Aldehyde Dehydrogenase, Hydroxyindoleacetic Acid, Liver/*drug effects/metabolism, Rats, 3. Good health, Dopamine/metabolism, Hydroxyindoleacetic Acid/metabolism, Chloramphenicol, Liver, Quinacrine/administration & dosage/pharmacology, 3,4-Dihydroxyphenylacetic Acid
Description: Several pharmaceutical agents produce ethanol intolerance, which is often depicted as disulfiram-like reaction. As in the case with disulfiram, the underlying mechanism is believed to be the accumulation of acetaldehyde in the blood, due to inhibition of the hepatic aldehyde dehydrogenases. In the present study, chloramphenicol, furazolidone, metronidazole, and quinacrine, which are reported to produce a disulfiram-like reaction, as well as disulfiram, were administered to Wistar rats and the hepatic activities of alcohol and aldehyde dehydrogenases (1A1 and 2) were determined. The expression of aldehyde dehydrogenase 2 was further assessed by Western blot analysis, while the levels of brain monoamines were also analyzed. Finally, blood acetaldehyde was evaluated after ethanol administration in rats pretreated with disulfiram, chloramphenicol, or quinacrine. The activity of aldehyde dehydrogenase 2 was inhibited by disulfiram, chloramphenicol, and furazolidone, but not by metronidazole or quinacrine. In addition, although well known for metronidazole, quinacrine also did not increase blood acetaldehyde after ethanol administration. The protein expression of aldehyde dehydrogenase 2 was not affected at all. Interestingly, all substances used, except disulfiram, increased the levels of brain serotonin. According to our findings, metronidazole and quinacrine do not produce a typical disulfiram-like reaction, because they do not inhibit hepatic aldehyde dehydrogenase nor increase blood acetaldehyde. Moreover, all tested agents share the common property to enhance brain serotonin, whereas a respective effect of ethanol is well established. Therefore, the ethanol intolerance produced by these agents, either aldehyde dehydrogenase is inhibited or not, could be the result of a “toxic serotonin syndrome,” as in the case of the concomitant use of serotonin-active medications.
Document Type: Article
Language: English
ISSN: 1092-874X
1091-5818
DOI: 10.1080/10915810701583010
Access URL: https://pubmed.ncbi.nlm.nih.gov/17963129
https://ijt.sagepub.com/content/26/5/423
http://journals.sagepub.com/doi/pdf/10.1080/10915810701583010
http://journals.sagepub.com/doi/10.1080/10915810701583010
https://journals.sagepub.com/doi/full/10.1080/10915810701583010
https://www.ncbi.nlm.nih.gov/pubmed/17963129
https://europepmc.org/article/MED/17963129
http://olympias.lib.uoi.gr/jspui/handle/123456789/19344
Rights: URL: https://journals.sagepub.com/page/policies/text-and-data-mining-license
Accession Number: edsair.doi.dedup.....bc3422c9b4afc9574e44c579078ed110
Database: OpenAIRE
Description
Abstract:Several pharmaceutical agents produce ethanol intolerance, which is often depicted as disulfiram-like reaction. As in the case with disulfiram, the underlying mechanism is believed to be the accumulation of acetaldehyde in the blood, due to inhibition of the hepatic aldehyde dehydrogenases. In the present study, chloramphenicol, furazolidone, metronidazole, and quinacrine, which are reported to produce a disulfiram-like reaction, as well as disulfiram, were administered to Wistar rats and the hepatic activities of alcohol and aldehyde dehydrogenases (1A1 and 2) were determined. The expression of aldehyde dehydrogenase 2 was further assessed by Western blot analysis, while the levels of brain monoamines were also analyzed. Finally, blood acetaldehyde was evaluated after ethanol administration in rats pretreated with disulfiram, chloramphenicol, or quinacrine. The activity of aldehyde dehydrogenase 2 was inhibited by disulfiram, chloramphenicol, and furazolidone, but not by metronidazole or quinacrine. In addition, although well known for metronidazole, quinacrine also did not increase blood acetaldehyde after ethanol administration. The protein expression of aldehyde dehydrogenase 2 was not affected at all. Interestingly, all substances used, except disulfiram, increased the levels of brain serotonin. According to our findings, metronidazole and quinacrine do not produce a typical disulfiram-like reaction, because they do not inhibit hepatic aldehyde dehydrogenase nor increase blood acetaldehyde. Moreover, all tested agents share the common property to enhance brain serotonin, whereas a respective effect of ethanol is well established. Therefore, the ethanol intolerance produced by these agents, either aldehyde dehydrogenase is inhibited or not, could be the result of a “toxic serotonin syndrome,” as in the case of the concomitant use of serotonin-active medications.
ISSN:1092874X
10915818
DOI:10.1080/10915810701583010