Arcopilins: A New Family of Staphylococcus aureus Biofilm Disruptors from the Soil Fungus Arcopilus navicularis
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| Název: | Arcopilins: A New Family of Staphylococcus aureus Biofilm Disruptors from the Soil Fungus Arcopilus navicularis |
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| Autoři: | Esteban Charria-Girón, Haoxuan Zeng, Tatiana E. Gorelik, Alexandra Pahl, Khai-Nghi Truong, Hedda Schrey, Frank Surup, Yasmina Marin-Felix |
| Zdroj: | J Med Chem |
| Informace o vydavateli: | American Chemical Society (ACS), 2024. |
| Rok vydání: | 2024 |
| Témata: | Staphylococcus aureus, Pyridones, Biofilms, Pyridones/chemistry [MeSH], Staphylococcus aureus/drug effects [MeSH], Pyridones/chemical synthesis [MeSH], Pyridones/pharmacology [MeSH], Pyrrolidinones/chemistry [MeSH], Pyrrolidinones/pharmacology [MeSH], Soil Microbiology [MeSH], Article, Biofilms/drug effects [MeSH], Anti-Bacterial Agents/chemistry [MeSH], Anti-Bacterial Agents/pharmacology [MeSH], Pyrrolidinones/metabolism [MeSH], Microbial Sensitivity Tests [MeSH], Microbial Sensitivity Tests, Soil Microbiology, Pyrrolidinones, Anti-Bacterial Agents |
| Popis: | Biofilms represent a key challenge in the treatment of microbial infections; for instance, Staphylococcus aureus causes chronic or fatal infections by forming biofilms on medical devices. Herein, the fungus Arcopilus navicularis was found to produce a novel family of PKS-NRPS metabolites that are able to disrupt preformed biofilms of S. aureus. Arcopilins A-F (1-6), tetramic acids, and arcopilin G (7), a 2-pyridone, were elucidated using HR-ESI-MS and one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy. Their absolute configuration was established by the synthesis of MPTA-esters for 2, analysis of 1H-1H coupling constants, and ROESY correlations, along with comparison with the crystal structure of 7. Arcopilin A (1) not only effectively disrupts preformed biofilms of S. aureus but also potentiates the activity of gentamicin and vancomycin up to 115- and 31-fold times, respectively. Our findings demonstrate the potential application of arcopilins for the conjugated treatment of infections caused by S. aureus with antibiotics unable to disrupt preformed biofilms. |
| Druh dokumentu: | Article Other literature type |
| Jazyk: | English |
| ISSN: | 1520-4804 0022-2623 |
| DOI: | 10.1021/acs.jmedchem.4c00585 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/39141525 https://repository.publisso.de/resource/frl:6486244 |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (http://creativecommons.org/licenses/by/4.0/). |
| Přístupové číslo: | edsair.doi.dedup.....bb8bda2f8e95873f630583ae4c3e8463 |
| Databáze: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | Biofilms represent a key challenge in the treatment of microbial infections; for instance, Staphylococcus aureus causes chronic or fatal infections by forming biofilms on medical devices. Herein, the fungus Arcopilus navicularis was found to produce a novel family of PKS-NRPS metabolites that are able to disrupt preformed biofilms of S. aureus. Arcopilins A-F (1-6), tetramic acids, and arcopilin G (7), a 2-pyridone, were elucidated using HR-ESI-MS and one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy. Their absolute configuration was established by the synthesis of MPTA-esters for 2, analysis of 1H-1H coupling constants, and ROESY correlations, along with comparison with the crystal structure of 7. Arcopilin A (1) not only effectively disrupts preformed biofilms of S. aureus but also potentiates the activity of gentamicin and vancomycin up to 115- and 31-fold times, respectively. Our findings demonstrate the potential application of arcopilins for the conjugated treatment of infections caused by S. aureus with antibiotics unable to disrupt preformed biofilms. |
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| ISSN: | 15204804 00222623 |
| DOI: | 10.1021/acs.jmedchem.4c00585 |