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Micro-architecture of calcium phosphate granules and fibrin glue composites for bone tissue engineering

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Title: Micro-architecture of calcium phosphate granules and fibrin glue composites for bone tissue engineering
Authors: Thierry Rouillon, Melitta Bilban, Guy Daculsi, Damien Le Nihouannen, Laurent Le Guehennec, Paul Pilet, Pierre Layrolle
Contributors: Layrolle, Pierre, Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR Odontologie (UFR Odonto), Université de Nantes (UN)-Université de Nantes (UN), Baxter BioSciences BioSurgery
Source: Biomaterials. 27:2716-2722
Publisher Information: Elsevier BV, 2006.
Publication Year: 2006
Subject Terms: MESH: Surface Properties, Calcium Phosphates, 0301 basic medicine, MESH: Molecular Conformation, MESH: Fibrin Tissue Adhesive, MESH: Materials Testing, Tissue Engineering, Surface Properties, Molecular Conformation, Thrombin, MESH: Bone Substitutes, Fibrin Tissue Adhesive, MESH: Calcium Phosphates, MESH: Tissue Engineering, MESH: Thrombin, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, 03 medical and health sciences, MESH: Porosity, Bone Substitutes, Materials Testing, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Porosity
Description: Calcium phosphate ceramics are currently used as bone graft substitutes in various types of clinical applications. Fibrin glue is also used in surgery due to its haemostatic, chemotactic and mitogenic properties. By combining these two biomaterials, new composite scaffolds were prepared. In this study, we attempt to analyse whether thrombin concentration in the fibrin glue could influence the properties of the composite. The association between fibrin glue and calcium phosphate ceramic granules was characterized at the ultra structural level. Micro and macroporous biphasic calcium phosphate ceramic granules with a diameter of 1-2mm composed of hydroxyapatite and beta-tricalcium phosphate (60/40) were associated to fibrin glue. The composites were observed by scanning and transmission electron microscopy and microcomputed tomography. Fibre thickness, porosity and homogeneity of the fibrin clot were modified by increased the thrombin concentration. Mixing fibrin glue with calcium phosphate granules (1:2) did not modify the microstructure of the fibrin clot in the composite. Nevertheless, thrombin interacted with the bioceramic by inducing the nucleation of crystalline precipitate at the ceramic/fibrin glue interface. Combining fibrin sealant and calcium phosphate ceramics could lead to new scaffolds for bone tissue engineering with the synergy of the properties of the two biomaterials.
Document Type: Article
Language: English
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2005.11.038
Access URL: https://pubmed.ncbi.nlm.nih.gov/16378638
https://www.sciencedirect.com/science/article/pii/S0142961205010653
https://www.ncbi.nlm.nih.gov/pubmed/16378638
http://www.sciencedirect.com/science/article/pii/S0142961205010653
https://inserm.hal.science/inserm-00166081v1
https://doi.org/10.1016/j.biomaterials.2005.11.038
Rights: Elsevier TDM
Accession Number: edsair.doi.dedup.....bb3fc5a78e8db70b11fc2d4fd338c09f
Database: OpenAIRE
Description
Abstract:Calcium phosphate ceramics are currently used as bone graft substitutes in various types of clinical applications. Fibrin glue is also used in surgery due to its haemostatic, chemotactic and mitogenic properties. By combining these two biomaterials, new composite scaffolds were prepared. In this study, we attempt to analyse whether thrombin concentration in the fibrin glue could influence the properties of the composite. The association between fibrin glue and calcium phosphate ceramic granules was characterized at the ultra structural level. Micro and macroporous biphasic calcium phosphate ceramic granules with a diameter of 1-2mm composed of hydroxyapatite and beta-tricalcium phosphate (60/40) were associated to fibrin glue. The composites were observed by scanning and transmission electron microscopy and microcomputed tomography. Fibre thickness, porosity and homogeneity of the fibrin clot were modified by increased the thrombin concentration. Mixing fibrin glue with calcium phosphate granules (1:2) did not modify the microstructure of the fibrin clot in the composite. Nevertheless, thrombin interacted with the bioceramic by inducing the nucleation of crystalline precipitate at the ceramic/fibrin glue interface. Combining fibrin sealant and calcium phosphate ceramics could lead to new scaffolds for bone tissue engineering with the synergy of the properties of the two biomaterials.
ISSN:01429612
DOI:10.1016/j.biomaterials.2005.11.038